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Q06546 (GABPA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 142. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GA-binding protein alpha chain

Short name=GABP subunit alpha
Alternative name(s):
Nuclear respiratory factor 2 subunit alpha
Transcription factor E4TF1-60
Gene names
Name:GABPA
Synonyms:E4TF1A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length454 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.

Subunit structure

Heterotetramer of two alpha and two beta subunits.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the ETS family.

Contains 1 ETS DNA-binding domain.

Contains 1 PNT (pointed) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityPolymorphism
   LigandDNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processin utero embryonic development

Inferred from electronic annotation. Source: Ensembl

negative regulation of megakaryocyte differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 22306510. Source: BHF-UCL

transcription from RNA polymerase II promoter

Traceable author statement Ref.1. Source: ProtInc

   Cellular_componentnuclear chromatin

Inferred from direct assay PubMed 22306510. Source: BHF-UCL

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionDNA binding

Traceable author statement Ref.1. Source: ProtInc

RNA polymerase II core promoter proximal region sequence-specific DNA binding

Inferred from direct assay PubMed 22306510. Source: BHF-UCL

RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 22306510. Source: BHF-UCL

chromatin binding

Inferred from electronic annotation. Source: Ensembl

protein heterodimerization activity

Inferred from direct assay PubMed 9857059. Source: MGI

sequence-specific DNA binding transcription factor activity

Traceable author statement PubMed 7590737Ref.1. Source: ProtInc

transcription coactivator activity

Traceable author statement Ref.1. Source: ProtInc

transcription regulatory region DNA binding

Inferred from direct assay PubMed 9857059. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HCFC1P516102EBI-638925,EBI-396176

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 454454GA-binding protein alpha chain
PRO_0000204127

Regions

Domain168 – 25184PNT
DNA binding320 – 40081ETS

Natural variations

Natural variant2911A → V.
Corresponds to variant rs2829897 [ dbSNP | Ensembl ].
VAR_020315
Natural variant3451E → K.
Corresponds to variant rs2829900 [ dbSNP | Ensembl ].
VAR_020316

Experimental info

Sequence conflict289 – 2902SS → RC in AAA65706. Ref.2
Sequence conflict4401A → V in AAA65706. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q06546 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 1AF2ABBBC79191DD

FASTA45451,295
        10         20         30         40         50         60 
MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYAPAECVSQ AIDINEPIGN LKKLLEPRLQ 

        70         80         90        100        110        120 
CSLDAHEICL QDIQLDPERS LFDQGVKTDG TVQLSVQVIS YQGIEPKLNI LEIVKPADTV 

       130        140        150        160        170        180 
EVVIDPDAHH AESEAHLVEE AQVITLDGTK HITTISDETS EQVTRWAAAL EGYRKEQERL 

       190        200        210        220        230        240 
GIPYDPIQWS TDQVLHWVVW VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL 

       250        260        270        280        290        300 
WSHLELLRKY VLASQEQQMN EIVTIDQPVQ IIPASVQSAT PTTIKVINSS AKAAKVQRAP 

       310        320        330        340        350        360 
RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL NQPELVAQKW 

       370        380        390        400        410        420 
GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV CDLKTLIGYS AAELNRLVTE 

       430        440        450 
CEQKKLAKMQ LHGIAQPVTA VALATASLQT EKDN 

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning of transcription factor E4TF1 subunits with Ets and notch motifs."
Watanabe H., Sawada J., Yano K., Yamaguchi K., Goto M., Handa H.
Mol. Cell. Biol. 13:1385-1391(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Four structurally distinct, non-DNA-binding subunits of human nuclear respiratory factor 2 share a conserved transcriptional activation domain."
Gugneja S., Virbasius J.V., Scarpulla R.C.
Mol. Cell. Biol. 15:102-111(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13318 mRNA. Translation: BAA02575.1.
U13044 mRNA. Translation: AAA65706.1.
PIRA48146.
I38739.
RefSeqNP_001184226.1. NM_001197297.1.
NP_002031.2. NM_002040.3.
XP_005260995.1. XM_005260938.2.
XP_005260996.1. XM_005260939.2.
UniGeneHs.473470.

3D structure databases

ProteinModelPortalQ06546.
SMRQ06546. Positions 35-121, 168-254, 320-429.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108826. 24 interactions.
IntActQ06546. 3 interactions.
MINTMINT-189695.
STRING9606.ENSP00000346886.

PTM databases

PhosphoSiteQ06546.

Polymorphism databases

DMDM729553.

Proteomic databases

PaxDbQ06546.
PeptideAtlasQ06546.
PRIDEQ06546.

Protocols and materials databases

DNASU2551.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000354828; ENSP00000346886; ENSG00000154727.
ENST00000400075; ENSP00000382948; ENSG00000154727.
GeneID2551.
KEGGhsa:2551.
UCSCuc002ylx.4. human.

Organism-specific databases

CTD2551.
GeneCardsGC21P027106.
HGNCHGNC:4071. GABPA.
HPACAB004411.
HPA003258.
HPA028791.
MIM600609. gene.
neXtProtNX_Q06546.
PharmGKBPA28485.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG252121.
HOGENOMHOG000285952.
HOVERGENHBG051687.
InParanoidQ06546.
KOK09441.
OMAGIPYDPV.
OrthoDBEOG7TXKGT.
PhylomeDBQ06546.
TreeFamTF350537.

Gene expression databases

ArrayExpressQ06546.
BgeeQ06546.
CleanExHS_GABPA.
GenevestigatorQ06546.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProIPR000418. Ets_dom.
IPR024668. GABP_asu_N.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR016312. TF_GA-bd_asu.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00178. Ets. 1 hit.
PF11620. GABP-alpha. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PIRSFPIRSF001703. GABP_alpha. 1 hit.
PRINTSPR00454. ETSDOMAIN.
SMARTSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
PROSITEPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGABPA. human.
GeneWikiGABPA.
GenomeRNAi2551.
NextBio10071.
PROQ06546.
SOURCESearch...

Entry information

Entry nameGABPA_HUMAN
AccessionPrimary (citable) accession number: Q06546
Secondary accession number(s): Q12939
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 21

Human chromosome 21: entries, gene names and cross-references to MIM