Reviewed, UniProtKB/Swiss-Prot Q06546 (GABPA_HUMAN)

Names and origin

Protein names

Recommended name:
    GA-binding protein alpha chain
      Short name=GABP subunit alpha
Alternative name(s):
    Transcription factor E4TF1-60
    Nuclear respiratory factor 2 subunit alpha

Gene names

Name:	GABPA
Synonyms:	E4TF1A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.
Subunit structure	Heterotetramer of two alpha and two beta subunits.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Gene Ontology (GO)
Biological process	transcription from RNA polymerase II promoter Ref.1 Traceable author statement. Source: ProtInc
Cellular component	nucleus Inferred from direct assay. Source: HPA
Molecular function	RNA polymerase II transcription factor activity Ref.1 Traceable author statement. Source: ProtInc sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription coactivator activity Ref.1 Traceable author statement. Source: ProtInc transcription factor activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HCFC1	P51610	1	EBI-638925,EBI-396176

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	GA-binding protein alpha chain		PRO_0000204127
Regions
	Domain	168 – 251	84	PNT
	DNA binding	320 – 400	81	ETS
Natural variations
	Natural variant	291	1	A → V: dbSNP rs2829897.		VAR_020315
	Natural variant	345	1	E → K: dbSNP rs2829900.		VAR_020316
Experimental info
	Sequence conflict	289 – 290	2	SS → RC in AAA65706. Ref.2
	Sequence conflict	440	1	A → V in AAA65706. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q06546-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 1AF2ABBBC79191DD	FASTA	454	51,295
	10 20 30 40 50 60 MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYAPAECVSQ AIDINEPIGN LKKLLEPRLQ 70 80 90 100 110 120 CSLDAHEICL QDIQLDPERS LFDQGVKTDG TVQLSVQVIS YQGIEPKLNI LEIVKPADTV 130 140 150 160 170 180 EVVIDPDAHH AESEAHLVEE AQVITLDGTK HITTISDETS EQVTRWAAAL EGYRKEQERL 190 200 210 220 230 240 GIPYDPIQWS TDQVLHWVVW VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL 250 260 270 280 290 300 WSHLELLRKY VLASQEQQMN EIVTIDQPVQ IIPASVQSAT PTTIKVINSS AKAAKVQRAP 310 320 330 340 350 360 RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL NQPELVAQKW 370 380 390 400 410 420 GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV CDLKTLIGYS AAELNRLVTE 430 440 450 CEQKKLAKMQ LHGIAQPVTA VALATASLQT EKDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of transcription factor E4TF1 subunits with Ets and notch motifs." Watanabe H., Sawada J., Yano K., Yamaguchi K., Goto M., Handa H. Mol. Cell. Biol. 13:1385-1391(1993) [PubMed: 8441384] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Four structurally distinct, non-DNA-binding subunits of human nuclear respiratory factor 2 share a conserved transcriptional activation domain." Gugneja S., Virbasius J.V., Scarpulla R.C. Mol. Cell. Biol. 15:102-111(1995) [PubMed: 7799916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13318 mRNA. Translation: BAA02575.1. U13044 mRNA. Translation: AAA65706.1.
IPI	IPI00299413.
PIR	A48146. I38739.
RefSeq	NP_002031.2.
UniGene	Hs.473470
3D structure databases
HSSP	HSSP built from PDB template 1AWC based on UniProtKB Q00422.
SMR	Q06546. Positions 168-254, 320-429.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q06546. 1 interaction.
PTM databases
PhosphoSite	Q06546.
Proteomic databases
PeptideAtlas	Q06546.
PRIDE	Q06546.
Genome annotation databases
Ensembl	ENSG00000154727. Homo sapiens. [Contig view]
GeneID	2551.
KEGG	hsa:2551.
Organism-specific databases
GeneCards	GC21P026028.
H-InvDB	HIX0027798. HIX0058426.
HGNC	HGNC:4071. GABPA.
HPA	CAB004411. HPA003258.
MIM	600609. gene.
PharmGKB	PA28485.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q06546.
HOVERGEN	Q06546.
OMA	Q06546. EERSSPG.
Gene expression databases
ArrayExpress	Q06546.
Bgee	Q06546.
CleanEx	HS_GABPA.
GermOnline	ENSG00000154727. Homo sapiens.
Family and domain databases
InterPro	IPR000418. Ets. IPR003118. SAM_PNT. IPR013761. SAM_type. IPR016312. TF_GA-bd_asu. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.150.50. SAM_type. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF00178. Ets. 1 hit. PF02198. SAM_PNT. 1 hit. [Graphical view]
PIRSF	PIRSF001703. GABP_alpha. 1 hit.
PRINTS	PR00454. ETSDOMAIN.
SMART	SM00413. ETS. 1 hit. SM00251. SAM_PNT. 1 hit. [Graphical view]
PROSITE	PS00345. ETS_DOMAIN_1. 1 hit. PS00346. ETS_DOMAIN_2. 1 hit. PS50061. ETS_DOMAIN_3. 1 hit. PS51433. PNT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	10071.
SOURCE	Search...

Entry information

Entry name

GABPA_HUMAN

Accession

Primary (citable) accession number: Q06546
Secondary accession number(s): Q12939

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)