Q06528 (CM4T_STRPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carminomycin 4-O-methyltransferase
Short name=COMT
EC=2.1.1.-

Gene names

Name:

dnrK

Organism

Streptomyces peucetius

Taxonomic identifier

1950 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of carminomycin to daunorubicin.
Pathway	Antibiotic biosynthesis; daunorubicin biosynthesis.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the methyltransferase superfamily.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW O-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 356

355

Carminomycin 4-O-methyltransferase

PRO_0000089881

Regions

Nucleotide binding

185 – 191

7

ATP Potential

Secondary structure

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356

Helix Strand Turn

Details...

Helix

14 – 24

11

Helix

27 – 37

11

Helix

40 – 45

6

Helix

51 – 58

8

Helix

62 – 74

13

Beta strand

77 – 82

6

Beta strand

85 – 88

4

Helix

92 – 95

4

Helix

103 – 106

4

Helix

112 – 116

5

Helix

117 – 122

6

Helix

123 – 129

7

Helix

134 – 138

5

Helix

142 – 147

6

Helix

150 – 160

11

Turn

161 – 168

8

Helix

169 – 174

6

Beta strand

181 – 186

6

Helix

192 – 200

9

Beta strand

205 – 210

6

Helix

214 – 224

11

Turn

228 – 230

3

Beta strand

231 – 235

5

Beta strand

246 – 253

8

Helix

255 – 257

3

Helix

260 – 272

13

Beta strand

274 – 284

11

Helix

289 – 291

3

Helix

295 – 308

14

Helix

315 – 324

10

Beta strand

327 – 336

10

Beta strand

338 – 341

4

Beta strand

343 – 351

9

Sequences

	Sequence		Length	Mass (Da)
	Q06528 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 2969EC5C6117ECF7	FASTA	356	38,782
	10 20 30 40 50 60 MTAEPTVAAR PQQIDALRTL IRLGSLHTPM VVRTAATLRL VDHILAGART VKALAARTDT 70 80 90 100 110 120 RPEALLRLIR HLVAIGLLEE DAPGEFVPTE VGELLADDHP AAQRAWHDLT QAVARADISF 130 140 150 160 170 180 TRLPDAIRTG RPTYESIYGK PFYEDLAGRP DLRASFDSLL ACDQDVAFDA PAAAYDWTNV 190 200 210 220 230 240 RHVLDVGGGK GGFAAAIARR APHVSATVLE MAGTVDTARS YLKDEGLSDR VDVVEGDFFE 250 260 270 280 290 300 PLPRKADAII LSFVLLNWPD HDAVRILTRC AEALEPGGRI LIHERDDLHE NSFNEQFTEL 310 320 330 340 350 DLRMLVFLGG ALRTREKWDG LAASAGLVVE EVRQLPSPTI PYDLSLLVLA PAATGA

References

[1]	"Cloning and sequencing of a gene encoding carminomycin 4-O-methyltransferase from Streptomyces peucetius and its expression in Escherichia coli." Madduri K., Torti F., Colombo A.L., Hutchinson C.R. J. Bacteriol. 175:3900-3904(1993) [PubMed: 8509343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-18. Strain: ATCC 29050 / DSM 40754 / JCM 9920 / NBRC 100596 / NCIMB 10972.
[2]	"Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius." Madduri K., Hutchinson C.R. J. Bacteriol. 177:3879-3884(1995) [PubMed: 7601857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29050 / DSM 40754 / JCM 9920 / NBRC 100596 / NCIMB 10972.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L13453 Genomic DNA. Translation: AAA26712.1.
L40425 Genomic DNA. Translation: AAA99001.1.

PIR

A47128.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TW2	X-ray	2.50	A/B	2-355	[»]
1TW3	X-ray	2.35	A/B	2-355	[»]

ProteinModelPortal

Q06528.

SMR

Q06528. Positions 4-353.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR016461. O-MeTrfase_COMT_euk.
IPR001077. O_MeTrfase_2.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

Pfam

PF00891. Methyltransf_2. 1 hit.
[Graphical view]

PIRSF

PIRSF005739. O-mtase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

CM4T_STRPE

Accession

Primary (citable) accession number: Q06528

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	November 30, 2010
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program