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Protein

Pre-mRNA-splicing factor URN1

Gene

URN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the spliceosome involved in mRNA processing.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-34283-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor URN1
Alternative name(s):
U2-U5-U6 snRNP, RES complex and NTC-interacting pre-mRNA-splicing factor 1
Gene namesi
Name:URN1
Ordered Locus Names:YPR152C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR152C.
SGDiS000006356. URN1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465Pre-mRNA-splicing factor URN1PRO_0000244477Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei150 – 1501PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06525.

PTM databases

iPTMnetiQ06525.

Interactioni

Subunit structurei

Component of the precatalytic spliceosomal complex B. Interacts with PRP19.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AIM4P383056EBI-35138,EBI-20939
PRP19P325237EBI-35138,EBI-493

Protein-protein interaction databases

BioGridi36319. 234 interactions.
DIPiDIP-2017N.
IntActiQ06525. 69 interactions.
MINTiMINT-401712.

Structurei

Secondary structure

1
465
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi216 – 22510Combined sources
Turni230 – 2323Combined sources
Helixi235 – 2417Combined sources
Beta strandi242 – 2443Combined sources
Helixi247 – 2493Combined sources
Helixi253 – 26412Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JUCNMR-A212-266[»]
ProteinModelPortaliQ06525.
SMRiQ06525. Positions 4-31, 55-87, 212-266.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06525.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 3232WWPROSITE-ProRule annotationAdd
BLAST
Domaini212 – 26655FFAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi119 – 19274Glu-richAdd
BLAST

Sequence similaritiesi

Contains 1 FF domain.Curated
Contains 1 WW domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000141866.
InParanoidiQ06525.
OMAiEPTKYHY.
OrthoDBiEOG7CVQ71.

Family and domain databases

Gene3Di1.10.10.440. 1 hit.
InterProiIPR002713. FF_domain.
IPR001202. WW_dom.
[Graphical view]
PfamiPF01846. FF. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00441. FF. 1 hit.
SM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF51045. SSF51045. 1 hit.
SSF81698. SSF81698. 1 hit.
PROSITEiPS51676. FF. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGEWQEFKT PAGKKYYYNK NTKQSRWEKP NLKKGSNLES NAKESQTERK
60 70 80 90 100
PTFSLELVNG WHLIIYNDGT KLYFNDDSKE FKNDISQEDD SRCRSLIESL
110 120 130 140 150
DKEKLVLLIG VARGYTMREE DIDKILESCN EEIHLFKRNQ DEVERKDEIS
160 170 180 190 200
EEAGDVKSPL QESHTGLVSG YGSSSGEEDE EEDEEEDEEN EEQIVNQDIS
210 220 230 240 250
IIDDLNRIDT DDIDERNIFF ELFDRYKLDK FSTWSLQSKK IENDPDFYKI
260 270 280 290 300
RDDTVRESLF EEWCGERSGN ATAEESDSED NSEDDSEVLE PTKYHYLAQI
310 320 330 340 350
VANAGTIAPD TIPQDIRKQQ KALYKAYKIK EYIPSKRDQD KFVSQLLFYY
360 370 380 390 400
KTFDLEQRKE IFCDCLRDHE RDFTGAVESL RQDKELIDRW QTLLKAPADS
410 420 430 440 450
SSIEDILLSI EHRCCVSPIV VTEPRYYVVG ILEKTVVWVR WLAAEVGPSS
460
RFTPVGAGNE PINPE
Length:465
Mass (Da):54,139
Last modified:November 1, 1996 - v1
Checksum:iB32285888B7DB772
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40829 Genomic DNA. Translation: AAB68289.1.
AY692740 Genomic DNA. Translation: AAT92759.1.
BK006949 Genomic DNA. Translation: DAA11564.1.
PIRiS69038.
RefSeqiNP_015478.1. NM_001184249.1.

Genome annotation databases

EnsemblFungiiYPR152C; YPR152C; YPR152C.
GeneIDi856275.
KEGGisce:YPR152C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40829 Genomic DNA. Translation: AAB68289.1.
AY692740 Genomic DNA. Translation: AAT92759.1.
BK006949 Genomic DNA. Translation: DAA11564.1.
PIRiS69038.
RefSeqiNP_015478.1. NM_001184249.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JUCNMR-A212-266[»]
ProteinModelPortaliQ06525.
SMRiQ06525. Positions 4-31, 55-87, 212-266.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36319. 234 interactions.
DIPiDIP-2017N.
IntActiQ06525. 69 interactions.
MINTiMINT-401712.

PTM databases

iPTMnetiQ06525.

Proteomic databases

MaxQBiQ06525.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR152C; YPR152C; YPR152C.
GeneIDi856275.
KEGGisce:YPR152C.

Organism-specific databases

EuPathDBiFungiDB:YPR152C.
SGDiS000006356. URN1.

Phylogenomic databases

HOGENOMiHOG000141866.
InParanoidiQ06525.
OMAiEPTKYHY.
OrthoDBiEOG7CVQ71.

Enzyme and pathway databases

BioCyciYEAST:G3O-34283-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ06525.
NextBioi981586.
PROiQ06525.

Family and domain databases

Gene3Di1.10.10.440. 1 hit.
InterProiIPR002713. FF_domain.
IPR001202. WW_dom.
[Graphical view]
PfamiPF01846. FF. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00441. FF. 1 hit.
SM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF51045. SSF51045. 1 hit.
SSF81698. SSF81698. 1 hit.
PROSITEiPS51676. FF. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Comparative analysis of Saccharomyces cerevisiae WW domains and their interacting proteins."
    Hesselberth J.R., Miller J.P., Golob A., Stajich J.E., Michaud G.A., Fields S.
    Genome Biol. 7:R30.1-R30.15(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-150, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Rapid screening of yeast mutants with reporters identifies new splicing phenotypes."
    Dreumont N., Seraphin B.
    FEBS J. 280:2712-2726(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome."
    Fabrizio P., Dannenberg J., Dube P., Kastner B., Stark H., Urlaub H., Luhrmann R.
    Mol. Cell 36:593-608(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PRECATALYTIC SPLICEOSOMAL COMPLEX B, IDENTIFICATION BY MASS SPECTROMETRY.
  10. "Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19."
    Ren L., McLean J.R., Hazbun T.R., Fields S., Vander Kooi C., Ohi M.D., Gould K.L.
    PLoS ONE 6:E16719-E16719(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRP19, FUNCTION.
  11. "Solution structure of the yeast URN1 splicing factor FF domain: comparative analysis of charge distributions in FF domain structures-FFs and SURPs, two domains with a similar fold."
    Bonet R., Ramirez-Espain X., Macias M.J.
    Proteins 73:1001-1009(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 212-266, DOMAIN.

Entry informationi

Entry nameiURN1_YEAST
AccessioniPrimary (citable) accession number: Q06525
Secondary accession number(s): D6W4E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1310 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.