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Protein

Bile salt sulfotransferase

Gene

SULT2A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands.

Catalytic activityi

3'-phosphoadenylyl sulfate + glycolithocholate = adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate.
3'-phosphoadenylyl sulfate + taurolithocholate = adenosine 3',5'-bisphosphate + taurolithocholate sulfate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72Substrate1
Binding sitei77Substrate1
Active sitei99Proton acceptor1
Binding sitei121PAPS1
Binding sitei129PAPS1
Binding sitei184PAPS1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 49PAPS6
Nucleotide bindingi218 – 223PAPS6
Nucleotide bindingi247 – 249PAPS3

GO - Molecular functioni

GO - Biological processi

  • 3'-phosphoadenosine 5'-phosphosulfate metabolic process Source: Reactome
  • bile acid catabolic process Source: UniProtKB-KW
  • digestion Source: ProtInc
  • steroid metabolic process Source: ProtInc
  • sulfation Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Bile acid catabolism, Lipid degradation, Lipid metabolism, Steroid metabolism

Enzyme and pathway databases

BioCyciZFISH:HS02732-MONOMER.
ReactomeiR-HSA-156584. Cytosolic sulfonation of small molecules.
R-HSA-1989781. PPARA activates gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Bile salt sulfotransferase (EC:2.8.2.14)
Alternative name(s):
Dehydroepiandrosterone sulfotransferase
Short name:
DHEA-ST
Hydroxysteroid Sulfotransferase
Short name:
HST
ST2
ST2A3
Sulfotransferase 2A1
Short name:
ST2A1
Gene namesi
Name:SULT2A1
Synonyms:HST, STD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:11458. SULT2A1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi6822.
OpenTargetsiENSG00000105398.
PharmGKBiPA346.

Chemistry databases

ChEMBLiCHEMBL2077.
DrugBankiDB05812. Abiraterone.
DB00316. Acetaminophen.

Polymorphism and mutation databases

BioMutaiSULT2A1.
DMDMi1711591.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000851411 – 285Bile salt sulfotransferaseAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06520.
PaxDbiQ06520.
PeptideAtlasiQ06520.
PRIDEiQ06520.

PTM databases

iPTMnetiQ06520.
PhosphoSitePlusiQ06520.

Expressioni

Tissue specificityi

Liver, adrenal and at lower level in the kidney. Is present in human fetus in higher level in the adrenal than the liver and the kidney.

Gene expression databases

BgeeiENSG00000105398.
CleanExiHS_SULT2A1.
ExpressionAtlasiQ06520. baseline and differential.
GenevisibleiQ06520. HS.

Organism-specific databases

HPAiCAB018755.
HPA041487.
HPA063633.

Interactioni

Subunit structurei

Homodimer.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
STAT3P407632EBI-3921363,EBI-518675
SULT1B1O437045EBI-3921363,EBI-10179062

Protein-protein interaction databases

BioGridi112691. 5 interactors.
IntActiQ06520. 5 interactors.
MINTiMINT-5002735.
STRINGi9606.ENSP00000222002.

Structurei

Secondary structure

1285
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Beta strandi11 – 13Combined sources3
Beta strandi15 – 17Combined sources3
Helixi20 – 28Combined sources9
Beta strandi37 – 40Combined sources4
Beta strandi43 – 46Combined sources4
Helixi47 – 58Combined sources12
Turni59 – 61Combined sources3
Helixi64 – 68Combined sources5
Helixi71 – 74Combined sources4
Helixi81 – 87Combined sources7
Beta strandi95 – 98Combined sources4
Helixi102 – 104Combined sources3
Helixi107 – 111Combined sources5
Beta strandi115 – 120Combined sources6
Helixi123 – 134Combined sources12
Beta strandi137 – 140Combined sources4
Helixi146 – 155Combined sources10
Helixi163 – 170Combined sources8
Helixi171 – 173Combined sources3
Beta strandi179 – 183Combined sources5
Helixi184 – 189Combined sources6
Helixi191 – 202Combined sources12
Helixi208 – 217Combined sources10
Helixi220 – 224Combined sources5
Turni227 – 229Combined sources3
Turni236 – 238Combined sources3
Beta strandi239 – 241Combined sources3
Helixi242 – 246Combined sources5
Helixi254 – 256Combined sources3
Helixi260 – 274Combined sources15
Helixi279 – 281Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EFHX-ray2.40A/B1-281[»]
1J99X-ray1.99A2-285[»]
1OV4X-ray2.70A2-285[»]
2QP3X-ray2.60A2-285[»]
2QP4X-ray3.00A2-285[»]
3F3YX-ray2.20A/B/C/D1-285[»]
4IFBX-ray2.30A/B1-285[»]
ProteinModelPortaliQ06520.
SMRiQ06520.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06520.

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Phylogenomic databases

eggNOGiKOG1584. Eukaryota.
ENOG4111H56. LUCA.
GeneTreeiENSGT00760000118932.
HOGENOMiHOG000037209.
HOVERGENiHBG001195.
InParanoidiQ06520.
KOiK11822.
OMAiPWVETEM.
OrthoDBiEOG091G0D5F.
PhylomeDBiQ06520.
TreeFamiTF321745.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06520-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDFLWFEG IAFPTMGFRS ETLRKVRDEF VIRDEDVIIL TYPKSGTNWL
60 70 80 90 100
AEILCLMHSK GDAKWIQSVP IWERSPWVES EIGYTALSET ESPRLFSSHL
110 120 130 140 150
PIQLFPKSFF SSKAKVIYLM RNPRDVLVSG YFFWKNMKFI KKPKSWEEYF
160 170 180 190 200
EWFCQGTVLY GSWFDHIHGW MPMREEKNFL LLSYEELKQD TGRTIEKICQ
210 220 230 240 250
FLGKTLEPEE LNLILKNSSF QSMKENKMSN YSLLSVDYVV DKAQLLRKGV
260 270 280
SGDWKNHFTV AQAEDFDKLF QEKMADLPRE LFPWE
Length:285
Mass (Da):33,780
Last modified:January 23, 2007 - v3
Checksum:i06DFF2006B284A08
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90T → S in AAA35758 (PubMed:7678732).Curated1
Sequence conflicti90T → S in CAA49755 (PubMed:7678732).Curated1
Sequence conflicti119L → D AA sequence (PubMed:7678732).Curated1
Sequence conflicti159L → V in AAB23169 (PubMed:1520333).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05252063A → P.1 PublicationCorresponds to variant rs11569681dbSNPEnsembl.1
Natural variantiVAR_052521261A → T.Corresponds to variant rs11569679dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20000 mRNA. Translation: AAA35758.1.
X70222 mRNA. Translation: CAA49755.1.
U08024 mRNA. Translation: AAA17749.1.
U08025 mRNA. Translation: AAA17750.1.
X84816 mRNA. Translation: CAA59274.1.
L36196
, L36191, L36192, L36193, L36194, L36195 Genomic DNA. Translation: AAA75491.1.
U13061
, U13056, U13057, U13058, U13059, U13060 Genomic DNA. Translation: AAC51353.1.
S43859 mRNA. Translation: AAB23169.2.
BC020755 mRNA. Translation: AAH20755.1.
CCDSiCCDS12707.1.
PIRiI53037. I38548.
RefSeqiNP_003158.2. NM_003167.3.
UniGeneiHs.515835.

Genome annotation databases

EnsembliENST00000222002; ENSP00000222002; ENSG00000105398.
GeneIDi6822.
KEGGihsa:6822.
UCSCiuc002phr.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20000 mRNA. Translation: AAA35758.1.
X70222 mRNA. Translation: CAA49755.1.
U08024 mRNA. Translation: AAA17749.1.
U08025 mRNA. Translation: AAA17750.1.
X84816 mRNA. Translation: CAA59274.1.
L36196
, L36191, L36192, L36193, L36194, L36195 Genomic DNA. Translation: AAA75491.1.
U13061
, U13056, U13057, U13058, U13059, U13060 Genomic DNA. Translation: AAC51353.1.
S43859 mRNA. Translation: AAB23169.2.
BC020755 mRNA. Translation: AAH20755.1.
CCDSiCCDS12707.1.
PIRiI53037. I38548.
RefSeqiNP_003158.2. NM_003167.3.
UniGeneiHs.515835.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EFHX-ray2.40A/B1-281[»]
1J99X-ray1.99A2-285[»]
1OV4X-ray2.70A2-285[»]
2QP3X-ray2.60A2-285[»]
2QP4X-ray3.00A2-285[»]
3F3YX-ray2.20A/B/C/D1-285[»]
4IFBX-ray2.30A/B1-285[»]
ProteinModelPortaliQ06520.
SMRiQ06520.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112691. 5 interactors.
IntActiQ06520. 5 interactors.
MINTiMINT-5002735.
STRINGi9606.ENSP00000222002.

Chemistry databases

ChEMBLiCHEMBL2077.
DrugBankiDB05812. Abiraterone.
DB00316. Acetaminophen.

PTM databases

iPTMnetiQ06520.
PhosphoSitePlusiQ06520.

Polymorphism and mutation databases

BioMutaiSULT2A1.
DMDMi1711591.

Proteomic databases

MaxQBiQ06520.
PaxDbiQ06520.
PeptideAtlasiQ06520.
PRIDEiQ06520.

Protocols and materials databases

DNASUi6822.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000222002; ENSP00000222002; ENSG00000105398.
GeneIDi6822.
KEGGihsa:6822.
UCSCiuc002phr.2. human.

Organism-specific databases

CTDi6822.
DisGeNETi6822.
GeneCardsiSULT2A1.
HGNCiHGNC:11458. SULT2A1.
HPAiCAB018755.
HPA041487.
HPA063633.
MIMi125263. gene.
neXtProtiNX_Q06520.
OpenTargetsiENSG00000105398.
PharmGKBiPA346.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1584. Eukaryota.
ENOG4111H56. LUCA.
GeneTreeiENSGT00760000118932.
HOGENOMiHOG000037209.
HOVERGENiHBG001195.
InParanoidiQ06520.
KOiK11822.
OMAiPWVETEM.
OrthoDBiEOG091G0D5F.
PhylomeDBiQ06520.
TreeFamiTF321745.

Enzyme and pathway databases

BioCyciZFISH:HS02732-MONOMER.
ReactomeiR-HSA-156584. Cytosolic sulfonation of small molecules.
R-HSA-1989781. PPARA activates gene expression.

Miscellaneous databases

ChiTaRSiSULT2A1. human.
EvolutionaryTraceiQ06520.
GeneWikiiBile_salt_sulfotransferase.
GenomeRNAii6822.
PROiQ06520.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000105398.
CleanExiHS_SULT2A1.
ExpressionAtlasiQ06520. baseline and differential.
GenevisibleiQ06520. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiST2A1_HUMAN
AccessioniPrimary (citable) accession number: Q06520
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 170 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Estrogens present in maternal circulation is predominantly derived from fetal dehydroepiandosterone sulfate which is hydrolyzed and metabolized to estrogens in placenta.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.