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Protein

Lysophosphatidylcholine acyltransferase

Gene

TAZ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyltransferase which mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a broad LPC fatty acyl chain substrate specificity utilizing LPC molecules ranging in length from C-10 to C-20. Does not utilize other lysolipids than LPC as acceptor. Also shows acyl-CoA-independent acyltransferase activity. Required for normal phospholipid content of mitochondrial membranes. Involved in the remodeling of the acyl groups of cardiolipin in the mitochondrial inner membrane, which affects the assembly and stability of respiratory chain complex IV and its supercomplex forms.3 Publications

Miscellaneous

Present with 1340 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine.

Enzyme regulationi

Inhibited by zinc and o-phenanthroline.1 Publication

Kineticsi

  1. KM=7 µM for lysophosphatidylcholine

    GO - Molecular functioni

    • 1-acylglycerophosphocholine O-acyltransferase activity Source: SGD

    GO - Biological processi

    • cardiolipin acyl-chain remodeling Source: SGD
    • cardiolipin metabolic process Source: SGD
    • inner mitochondrial membrane organization Source: SGD
    • mitochondrial ATP synthesis coupled electron transport Source: SGD
    • mitochondrial respiratory chain supercomplex assembly Source: SGD
    • phospholipid biosynthetic process Source: SGD

    Keywordsi

    Molecular functionAcyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-34275-MONOMER
    YEAST:G3O-34275-MONOMER
    ReactomeiR-SCE-1482798 Acyl chain remodeling of CL

    Chemistry databases

    SwissLipidsiSLP:000000064

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysophosphatidylcholine acyltransferase (EC:2.3.1.23)
    Alternative name(s):
    1-acylglycerophosphocholine O-acyltransferase
    Tafazzin homolog
    lyso-PC acyltransferase
    Gene namesi
    Name:TAZ1
    Ordered Locus Names:YPR140W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XVI

    Organism-specific databases

    EuPathDBiFungiDB:YPR140W
    SGDiS000006344 TAZ1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
    Transmembranei26 – 47HelicalSequence analysisAdd BLAST22
    Topological domaini48 – 381Mitochondrial intermembraneSequence analysisAdd BLAST334

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002209341 – 381Lysophosphatidylcholine acyltransferaseAdd BLAST381

    Proteomic databases

    MaxQBiQ06510
    PaxDbiQ06510
    PRIDEiQ06510

    Interactioni

    Protein-protein interaction databases

    BioGridi36309, 84 interactors
    MINTiQ06510
    STRINGi4932.YPR140W

    Structurei

    3D structure databases

    ProteinModelPortaliQ06510
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi77 – 82HXXXXD motif6

    Domaini

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine.By similarity

    Sequence similaritiesi

    Belongs to the taffazin family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00390000018621
    HOGENOMiHOG000248209
    InParanoidiQ06510
    KOiK13511
    OMAiGAHDICF
    OrthoDBiEOG092C2I7W

    Family and domain databases

    InterProiView protein in InterPro
    IPR002123 Plipid/glycerol_acylTrfase
    IPR000872 Tafazzin
    PANTHERiPTHR12497 PTHR12497, 1 hit
    PfamiView protein in Pfam
    PF01553 Acyltransferase, 1 hit
    PRINTSiPR00979 TAFAZZIN
    SMARTiView protein in SMART
    SM00563 PlsC, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q06510-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSFRDVLERG DEFLEAYPRR SPLWRFLSYS TSLLTFGVSK LLLFTCYNVK
    60 70 80 90 100
    LNGFEKLETA LERSKRENRG LMTVMNHMSM VDDPLVWATL PYKLFTSLDN
    110 120 130 140 150
    IRWSLGAHNI CFQNKFLANF FSLGQVLSTE RFGVGPFQGS IDASIRLLSP
    160 170 180 190 200
    DDTLDLEWTP HSEVSSSLKK AYSPPIIRSK PSWVHVYPEG FVLQLYPPFE
    210 220 230 240 250
    NSMRYFKWGI TRMILEATKP PIVVPIFATG FEKIASEAVT DSMFRQILPR
    260 270 280 290 300
    NFGSEINVTI GDPLNDDLID RYRKEWTHLV EKYYDPKNPN DLSDELKYGK
    310 320 330 340 350
    EAQDLRSRLA AELRAHVAEI RNEVRKLPRE DPRFKSPSWW KRFNTTEGKS
    360 370 380
    DPDVKVIGEN WAIRRMQKFL PPEGKPKGKD D
    Length:381
    Mass (Da):44,188
    Last modified:November 1, 1996 - v1
    Checksum:i76964FA6C77989B2
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti11D → Y in AAT92758 (PubMed:17322287).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U40829 Genomic DNA Translation: AAB68280.1
    AY692739 Genomic DNA Translation: AAT92758.1
    BK006949 Genomic DNA Translation: DAA11554.1
    PIRiS69029
    RefSeqiNP_015466.1, NM_001184237.1

    Genome annotation databases

    EnsemblFungiiYPR140W; YPR140W; YPR140W
    GeneIDi856262
    KEGGisce:YPR140W

    Similar proteinsi

    Entry informationi

    Entry nameiTAZ1_YEAST
    AccessioniPrimary (citable) accession number: Q06510
    Secondary accession number(s): D6W4D8, Q6B2J1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2004
    Last sequence update: November 1, 1996
    Last modified: May 23, 2018
    This is version 138 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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