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Protein

Lysophosphatidylcholine acyltransferase

Gene

TAZ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acyltransferase which mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a broad LPC fatty acyl chain substrate specificity utilizing LPC molecules ranging in length from C-10 to C-20. Does not utilize other lysolipids than LPC as acceptor. Also shows acyl-CoA-independent acyltransferase activity. Required for normal phospholipid content of mitochondrial membranes. Involved in the remodeling of the acyl groups of cardiolipin in the mitochondrial inner membrane, which affects the assembly and stability of respiratory chain complex IV and its supercomplex forms.3 Publications

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine.

Enzyme regulationi

Inhibited by zinc and o-phenanthroline.1 Publication

Kineticsi

  1. KM=7 µM for lysophosphatidylcholine

    GO - Molecular functioni

    • 1-acylglycerophosphocholine O-acyltransferase activity Source: SGD

    GO - Biological processi

    • cardiolipin acyl-chain remodeling Source: SGD
    • cardiolipin metabolic process Source: SGD
    • inner mitochondrial membrane organization Source: SGD
    • mitochondrial ATP synthesis coupled electron transport Source: SGD
    • phospholipid biosynthetic process Source: SGD
    • protein complex assembly Source: SGD
    • protein targeting to mitochondrion Source: Reactome
    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-34275-MONOMER.
    YEAST:G3O-34275-MONOMER.
    ReactomeiR-SCE-1268020. Mitochondrial protein import.
    R-SCE-1482798. Acyl chain remodeling of CL.

    Chemistry databases

    SwissLipidsiSLP:000000064.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysophosphatidylcholine acyltransferase (EC:2.3.1.23)
    Alternative name(s):
    1-acylglycerophosphocholine O-acyltransferase
    Tafazzin homolog
    lyso-PC acyltransferase
    Gene namesi
    Name:TAZ1
    Ordered Locus Names:YPR140W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XVI

    Organism-specific databases

    EuPathDBiFungiDB:YPR140W.
    SGDiS000006344. TAZ1.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
    Transmembranei26 – 47HelicalSequence analysisAdd BLAST22
    Topological domaini48 – 381Mitochondrial intermembraneSequence analysisAdd BLAST334

    GO - Cellular componenti

    • integral component of mitochondrial outer membrane Source: SGD
    • mitochondrial inner membrane Source: SGD
    • mitochondrial intermembrane space Source: Reactome
    • mitochondrial outer membrane Source: SGD
    • mitochondrion Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002209341 – 381Lysophosphatidylcholine acyltransferaseAdd BLAST381

    Proteomic databases

    MaxQBiQ06510.
    PRIDEiQ06510.

    Interactioni

    Protein-protein interaction databases

    BioGridi36309. 62 interactors.
    MINTiMINT-2492765.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06510.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi77 – 82HXXXXD motif6

    Domaini

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine.By similarity

    Sequence similaritiesi

    Belongs to the taffazin family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00390000018621.
    HOGENOMiHOG000248209.
    InParanoidiQ06510.
    KOiK13511.
    OMAiFFTMGQV.
    OrthoDBiEOG092C2I7W.

    Family and domain databases

    InterProiIPR002123. Plipid/glycerol_acylTrfase.
    IPR000872. Tafazzin.
    [Graphical view]
    PANTHERiPTHR12497. PTHR12497. 2 hits.
    PfamiPF01553. Acyltransferase. 1 hit.
    [Graphical view]
    PRINTSiPR00979. TAFAZZIN.
    SMARTiSM00563. PlsC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q06510-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSFRDVLERG DEFLEAYPRR SPLWRFLSYS TSLLTFGVSK LLLFTCYNVK
    60 70 80 90 100
    LNGFEKLETA LERSKRENRG LMTVMNHMSM VDDPLVWATL PYKLFTSLDN
    110 120 130 140 150
    IRWSLGAHNI CFQNKFLANF FSLGQVLSTE RFGVGPFQGS IDASIRLLSP
    160 170 180 190 200
    DDTLDLEWTP HSEVSSSLKK AYSPPIIRSK PSWVHVYPEG FVLQLYPPFE
    210 220 230 240 250
    NSMRYFKWGI TRMILEATKP PIVVPIFATG FEKIASEAVT DSMFRQILPR
    260 270 280 290 300
    NFGSEINVTI GDPLNDDLID RYRKEWTHLV EKYYDPKNPN DLSDELKYGK
    310 320 330 340 350
    EAQDLRSRLA AELRAHVAEI RNEVRKLPRE DPRFKSPSWW KRFNTTEGKS
    360 370 380
    DPDVKVIGEN WAIRRMQKFL PPEGKPKGKD D
    Length:381
    Mass (Da):44,188
    Last modified:November 1, 1996 - v1
    Checksum:i76964FA6C77989B2
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti11D → Y in AAT92758 (PubMed:17322287).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U40829 Genomic DNA. Translation: AAB68280.1.
    AY692739 Genomic DNA. Translation: AAT92758.1.
    BK006949 Genomic DNA. Translation: DAA11554.1.
    PIRiS69029.
    RefSeqiNP_015466.1. NM_001184237.1.

    Genome annotation databases

    EnsemblFungiiYPR140W; YPR140W; YPR140W.
    GeneIDi856262.
    KEGGisce:YPR140W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U40829 Genomic DNA. Translation: AAB68280.1.
    AY692739 Genomic DNA. Translation: AAT92758.1.
    BK006949 Genomic DNA. Translation: DAA11554.1.
    PIRiS69029.
    RefSeqiNP_015466.1. NM_001184237.1.

    3D structure databases

    ProteinModelPortaliQ06510.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi36309. 62 interactors.
    MINTiMINT-2492765.

    Chemistry databases

    SwissLipidsiSLP:000000064.

    Proteomic databases

    MaxQBiQ06510.
    PRIDEiQ06510.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYPR140W; YPR140W; YPR140W.
    GeneIDi856262.
    KEGGisce:YPR140W.

    Organism-specific databases

    EuPathDBiFungiDB:YPR140W.
    SGDiS000006344. TAZ1.

    Phylogenomic databases

    GeneTreeiENSGT00390000018621.
    HOGENOMiHOG000248209.
    InParanoidiQ06510.
    KOiK13511.
    OMAiFFTMGQV.
    OrthoDBiEOG092C2I7W.

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-34275-MONOMER.
    YEAST:G3O-34275-MONOMER.
    ReactomeiR-SCE-1268020. Mitochondrial protein import.
    R-SCE-1482798. Acyl chain remodeling of CL.

    Miscellaneous databases

    PROiQ06510.

    Family and domain databases

    InterProiIPR002123. Plipid/glycerol_acylTrfase.
    IPR000872. Tafazzin.
    [Graphical view]
    PANTHERiPTHR12497. PTHR12497. 2 hits.
    PfamiPF01553. Acyltransferase. 1 hit.
    [Graphical view]
    PRINTSiPR00979. TAFAZZIN.
    SMARTiSM00563. PlsC. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiTAZ1_YEAST
    AccessioniPrimary (citable) accession number: Q06510
    Secondary accession number(s): D6W4D8, Q6B2J1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2004
    Last sequence update: November 1, 1996
    Last modified: November 2, 2016
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1340 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.