ID   COMT1_MAIZE             Reviewed;         364 AA.
AC   Q06509;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   20-JAN-2009, entry version 44.
DE   RecName: Full=Caffeic acid 3-O-methyltransferase;
DE            EC=2.1.1.68;
DE   AltName: Full=S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferase;
DE            Short=CAOMT;
DE            Short=COMT;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae;
OC   PACCAD clade; Panicoideae; Andropogoneae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=93099238; PubMed=1463825; DOI=10.1007/BF00027157;
RA   Collazo P., Montoliu L., Puigdomenech P., Rigau J.;
RT   "Structure and expression of the lignin O-methyltransferase gene from
RT   Zea mays L.";
RL   Plant Mol. Biol. 20:857-867(1992).
CC   -!- FUNCTION: Catalyzes the conversion of caffeic acid to ferulic acid
CC       and of 5-hydroxyferulic acid to sinapic acid. The resulting
CC       products may subsequently be converted to the corresponding
CC       alcohols that are incorporated into lignins.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + 3,4-dihydroxy-trans-
CC       cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-
CC       cinnamate.
CC   -!- PATHWAY: Aromatic compound metabolism; phenylpropanoid
CC       biosynthesis.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. Type 2
CC       family. COMT subfamily.
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DR   EMBL; M73235; AAB03364.1; -; Genomic_DNA.
DR   PIR; S28612; S28612.
DR   HSSP; P28002; 1KYZ.
DR   Gramene; Q06509; -.
DR   MaizeGDB; 26027; -.
DR   BRENDA; 2.1.1.68; 289.
DR   GO; GO:0047763; F:caffeate O-methyltransferase activity; IEA:EC.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0009809; P:lignin biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR016461; O-MeTrfase_COMT_euk.
DR   InterPro; IPR001077; O_MeTrfase_2.
DR   InterPro; IPR012967; Plant_MeTrfase_dimerisation.
DR   InterPro; IPR011991; Wing_hlx_DNA_bd.
DR   Gene3D; G3DSA:1.10.10.10; Wing_hlx_DNA_bd; 1.
DR   Pfam; PF08100; Dimerisation; 1.
DR   Pfam; PF00891; Methyltransf_2; 1.
DR   PIRSF; PIRSF005739; O-mtase; 1.
PE   3: Inferred from homology;
KW   Lignin biosynthesis; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN         1    364       Caffeic acid 3-O-methyltransferase.
FT                                /FTId=PRO_0000063203.
FT   REGION      131    137       Substrate binding (By similarity).
FT   REGION      163    181       Substrate binding (By similarity).
FT   ACT_SITE    270    270       Proton acceptor (By similarity).
FT   BINDING     209    209       S-adenosyl-L-methionine; via carbonyl
FT                                oxygen (By similarity).
FT   BINDING     232    232       S-adenosyl-L-methionine (By similarity).
FT   BINDING     252    252       S-adenosyl-L-methionine (By similarity).
FT   BINDING     253    253       S-adenosyl-L-methionine; via amide
FT                                nitrogen (By similarity).
FT   BINDING     266    266       S-adenosyl-L-methionine (By similarity).
SQ   SEQUENCE   364 AA;  39567 MW;  089E8A6A4DDAF56A CRC64;
     MGSTAGDVAA VVDEEACMYA MQLASSSILP MTLKNAIELG LLEVLQKEAG GGKAALAPEE
     VVARMPAAPS DPAAAAAMVD RMLRLLASYD VVRCQMEDRD GRYERRYSAA PVCKWLTPNE
     DGVSMAALAL MNQDKVLMES WYYLKDAVLD GGIPFNKAYG MTAFEYHGTD ARFNRVFNEG
     MKNHSVIITK KLLDFYTGFE GVSTLVDVGG GVGATLHAIT SRHPHISGVN FDLPHVISEA
     PPFPGVRHVG GDMFASVPAG DAILMKWILH DWSDAHCATL LKNCYDALPE NGKVIVVECV
     LPVNTEATPK AQGVFHVDMI MLAHNPGGKE RYEREFRELA KGAGFSGFKA TYIYANAWAI
     EFIK
//