Q06509 (COMT1_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Caffeic acid 3-O-methyltransferase Short name=CAOMT Short name=COMT EC=2.1.1.68 Alternative name(s): S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferase
Organism	Zea mays (Maize)
Taxonomic identifier	4577 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid. The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins.
Catalytic activity	S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate.
Pathway	Aromatic compound metabolism; phenylpropanoid biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. Type 2 family. COMT subfamily.

Ontologies

Keywords
Biological process	Lignin biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	lignin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	caffeate O-methyltransferase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Caffeic acid 3-O-methyltransferase		PRO_0000063203
Regions
	Region	131 – 137	7	Substrate binding By similarity
	Region	163 – 181	19	Substrate binding By similarity
Sites
	Active site	270	1	Proton acceptor By similarity
	Binding site	209	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	232	1	S-adenosyl-L-methionine By similarity
	Binding site	252	1	S-adenosyl-L-methionine By similarity
	Binding site	253	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	266	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06509 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 089E8A6A4DDAF56A	FASTA	364	39,567
	10 20 30 40 50 60 MGSTAGDVAA VVDEEACMYA MQLASSSILP MTLKNAIELG LLEVLQKEAG GGKAALAPEE 70 80 90 100 110 120 VVARMPAAPS DPAAAAAMVD RMLRLLASYD VVRCQMEDRD GRYERRYSAA PVCKWLTPNE 130 140 150 160 170 180 DGVSMAALAL MNQDKVLMES WYYLKDAVLD GGIPFNKAYG MTAFEYHGTD ARFNRVFNEG 190 200 210 220 230 240 MKNHSVIITK KLLDFYTGFE GVSTLVDVGG GVGATLHAIT SRHPHISGVN FDLPHVISEA 250 260 270 280 290 300 PPFPGVRHVG GDMFASVPAG DAILMKWILH DWSDAHCATL LKNCYDALPE NGKVIVVECV 310 320 330 340 350 360 LPVNTEATPK AQGVFHVDMI MLAHNPGGKE RYEREFRELA KGAGFSGFKA TYIYANAWAI EFIK

References

[1]	"Structure and expression of the lignin O-methyltransferase gene from Zea mays L." Collazo P., Montoliu L., Puigdomenech P., Rigau J. Plant Mol. Biol. 20:857-867(1992) [PubMed: 1463825] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M73235 Genomic DNA. Translation: AAB03364.1.
PIR	S28612.
3D structure databases
ProteinModelPortal	Q06509.
SMR	Q06509. Positions 2-364.
ModBase	Search...
Proteomic databases
PRIDE	Q06509.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q06509.
MaizeGDB	26027.
Family and domain databases
InterPro	IPR022657. De-COase2_CS. IPR016461. O-MeTrfase_COMT_euk. IPR001077. O_MeTrfase_2. IPR012967. Plant_MeTrfase_dimerisation. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF08100. Dimerisation. 1 hit. PF00891. Methyltransf_2. 1 hit. [Graphical view]
PIRSF	PIRSF005739. O-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COMT1_MAIZE

Accession

Primary (citable) accession number: Q06509

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 16, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program