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Protein

N-terminal acetyltransferase B complex catalytic subunit NAT3

Gene

NAT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn, Met-Gln or Met-Met. NatB acetylates TPM1 protein and regulates tropomyocin-actin interactions, it is presumed to N-acetylate 15% of all yeast proteins.3 Publications

Miscellaneous

Present with 639 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein] = an N-terminal N(alpha)-acetyl-L-methionyl-L-asparginyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein] = an N-terminal N(alpha)-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein] = an N-terminal N(alpha)-acetyl-L-methionyl-L-aspartyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein] = an N-terminal N(alpha)-acetyl-L-methionyl-L-glutamyl-[protein] + CoA.1 Publication

GO - Molecular functioni

  • peptide alpha-N-acetyltransferase activity Source: SGD

GO - Biological processi

  • mitochondrion inheritance Source: SGD
  • N-terminal peptidyl-methionine acetylation Source: SGD
  • regulation of actin cytoskeleton organization Source: SGD
  • ubiquitin-dependent ERAD pathway Source: SGD

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-34267-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal acetyltransferase B complex catalytic subunit NAT3 (EC:2.3.1.2541 Publication)
Short name:
NatB complex subunit NAT3
Alternative name(s):
NatB Nalpha terminal acetyltransferase 3
Gene namesi
Name:NAT3Imported
Ordered Locus Names:YPR131C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR131C
SGDiS000006335 NAT3

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000746351 – 195N-terminal acetyltransferase B complex catalytic subunit NAT3Add BLAST195

Proteomic databases

MaxQBiQ06504
PaxDbiQ06504
PRIDEiQ06504

Interactioni

Subunit structurei

Component of the N-terminal acetyltransferase B (NatB) complex, which is composed of NAT3 and MDM20.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MDM20Q123877EBI-31002,EBI-5736

Protein-protein interaction databases

BioGridi36297, 34 interactors
IntActiQ06504, 8 interactors
MINTiQ06504
STRINGi4932.YPR131C

Structurei

3D structure databases

ProteinModelPortaliQ06504
SMRiQ06504
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 172N-acetyltransferasePROSITE-ProRule annotationAdd BLAST171

Sequence similaritiesi

Belongs to the acetyltransferase family. GNAT subfamily.Sequence analysis

Phylogenomic databases

GeneTreeiENSGT00550000075046
HOGENOMiHOG000078523
InParanoidiQ06504
KOiK17972
OMAiWTETYGI
OrthoDBiEOG092C4MWD

Family and domain databases

InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR000182 GNAT_dom
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51186 GNAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q06504-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTIQPFEPV DLFKTNNVNL DILTENFPLE FYFEYMIIWP DLFFKSSEMT
60 70 80 90 100
VDPTFKHNIS GYMMAKTEGK TTEWHTHITA VTVAPRFRRI SLASKLCNTL
110 120 130 140 150
ETMTDVMPHE VNFIDLFVKC NNQLAIKLYE KLGYSVYRRV VGYYNSAEDG
160 170 180 190
YPDTLKKVDD NKDAFDMRKA MARDRNRSVR PDGRSHKCYP HDVRF
Length:195
Mass (Da):22,921
Last modified:December 6, 2005 - v2
Checksum:i093EC599E326D338
GO

Sequence cautioni

The sequence AAB68272 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40829 Genomic DNA Translation: AAB68272.1 Different initiation.
BK006949 Genomic DNA Translation: DAA11543.1
PIRiS69021
RefSeqiNP_015456.2, NM_001184228.1

Genome annotation databases

EnsemblFungiiYPR131C; YPR131C; YPR131C
GeneIDi856249
KEGGisce:YPR131C

Similar proteinsi

Entry informationi

Entry nameiNAT3_YEAST
AccessioniPrimary (citable) accession number: Q06504
Secondary accession number(s): D6W4C7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health