Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N-terminal acetyltransferase B complex catalytic subunit NAT3

Gene

NAT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn, Met-Gln or Met-Met. NatB acetylates TPM1 protein and regulates tropomyocin-actin interactions, it is presumed to N-acetylate 15% of all yeast proteins.3 Publications

Catalytic activityi

Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA.

GO - Molecular functioni

  • peptide alpha-N-acetyltransferase activity Source: SGD

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • mitochondrion inheritance Source: SGD
  • N-terminal peptidyl-methionine acetylation Source: SGD
  • regulation of actin cytoskeleton organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-34267-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal acetyltransferase B complex catalytic subunit NAT3 (EC:2.3.1.88)
Short name:
NatB complex subunit NAT3
Alternative name(s):
NatB Nalpha terminal acetyltransferase 3
Gene namesi
Name:NAT3Imported
Ordered Locus Names:YPR131C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR131C.
SGDiS000006335. NAT3.

Subcellular locationi

GO - Cellular componenti

  • NatB complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000746351 – 195N-terminal acetyltransferase B complex catalytic subunit NAT3Add BLAST195

Proteomic databases

MaxQBiQ06504.
PRIDEiQ06504.

Interactioni

Subunit structurei

Component of the N-terminal acetyltransferase B (NatB) complex, which is composed of NAT3 and MDM20.1 Publication

Protein-protein interaction databases

BioGridi36297. 22 interactors.
IntActiQ06504. 1 interactor.
MINTiMINT-2785477.

Structurei

3D structure databases

ProteinModelPortaliQ06504.
SMRiQ06504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 172N-acetyltransferasePROSITE-ProRule annotationAdd BLAST171

Sequence similaritiesi

Belongs to the acetyltransferase family. GNAT subfamily.Sequence analysis
Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000075046.
HOGENOMiHOG000078523.
InParanoidiQ06504.
KOiK17972.
OMAiTEWHTHI.
OrthoDBiEOG092C4MWD.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06504-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTIQPFEPV DLFKTNNVNL DILTENFPLE FYFEYMIIWP DLFFKSSEMT
60 70 80 90 100
VDPTFKHNIS GYMMAKTEGK TTEWHTHITA VTVAPRFRRI SLASKLCNTL
110 120 130 140 150
ETMTDVMPHE VNFIDLFVKC NNQLAIKLYE KLGYSVYRRV VGYYNSAEDG
160 170 180 190
YPDTLKKVDD NKDAFDMRKA MARDRNRSVR PDGRSHKCYP HDVRF
Length:195
Mass (Da):22,921
Last modified:December 6, 2005 - v2
Checksum:i093EC599E326D338
GO

Sequence cautioni

The sequence AAB68272 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40829 Genomic DNA. Translation: AAB68272.1. Different initiation.
BK006949 Genomic DNA. Translation: DAA11543.1.
PIRiS69021.
RefSeqiNP_015456.2. NM_001184228.1.

Genome annotation databases

EnsemblFungiiYPR131C; YPR131C; YPR131C.
GeneIDi856249.
KEGGisce:YPR131C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40829 Genomic DNA. Translation: AAB68272.1. Different initiation.
BK006949 Genomic DNA. Translation: DAA11543.1.
PIRiS69021.
RefSeqiNP_015456.2. NM_001184228.1.

3D structure databases

ProteinModelPortaliQ06504.
SMRiQ06504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36297. 22 interactors.
IntActiQ06504. 1 interactor.
MINTiMINT-2785477.

Proteomic databases

MaxQBiQ06504.
PRIDEiQ06504.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR131C; YPR131C; YPR131C.
GeneIDi856249.
KEGGisce:YPR131C.

Organism-specific databases

EuPathDBiFungiDB:YPR131C.
SGDiS000006335. NAT3.

Phylogenomic databases

GeneTreeiENSGT00550000075046.
HOGENOMiHOG000078523.
InParanoidiQ06504.
KOiK17972.
OMAiTEWHTHI.
OrthoDBiEOG092C4MWD.

Enzyme and pathway databases

BioCyciYEAST:G3O-34267-MONOMER.

Miscellaneous databases

PROiQ06504.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAT3_YEAST
AccessioniPrimary (citable) accession number: Q06504
Secondary accession number(s): D6W4C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 639 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.