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Protein

Peroxisomal adenine nucleotide transporter 1

Gene

ANT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Adenine nucleotide transporter involved in the uniport of ATP and adenine nucleotide hetero-exchange transport between the cytosol and the peroxisomal lumen. This transport is accompanied by a proton transport from the peroxisomal lumen to the cytosol. Transport of ATP into the peroxisome is required for beta-oxidation of medium-chain fatty acids. Required for growth on medium-chain fatty acids, pH gradient formation in peroxisomes and for normal peroxisome proliferation.4 Publications

GO - Molecular functioni

  • adenine nucleotide transmembrane transporter activity Source: SGD

GO - Biological processi

  • ATP transport Source: SGD
  • fatty acid beta-oxidation Source: SGD
  • peroxisome organization Source: SGD
  • proton transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34265-MONOMER.

Protein family/group databases

TCDBi2.A.29.17.1. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal adenine nucleotide transporter 1
Gene namesi
Name:ANT1
Ordered Locus Names:YPR128C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR128C.
SGDiS000006332. ANT1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121Helical; Name=1Sequence analysisAdd
BLAST
Transmembranei78 – 9821Helical; Name=2Sequence analysisAdd
BLAST
Transmembranei128 – 14821Helical; Name=3Sequence analysisAdd
BLAST
Transmembranei185 – 20218Helical; Name=4Sequence analysisAdd
BLAST
Transmembranei226 – 24621Helical; Name=5Sequence analysisAdd
BLAST
Transmembranei277 – 29721Helical; Name=6Sequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of peroxisomal membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Peroxisomal adenine nucleotide transporter 1PRO_0000227603Add
BLAST

Proteomic databases

MaxQBiQ06497.
PeptideAtlasiQ06497.

Expressioni

Inductioni

Induced by oleic acid, through its ORE-like (oleate responsive element) promoter element and the PIP2-OAF1 transcription factors.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi36295. 39 interactions.
DIPiDIP-8825N.
IntActiQ06497. 2 interactions.
MINTiMINT-4506416.

Structurei

3D structure databases

ProteinModelPortaliQ06497.
SMRiQ06497. Positions 4-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 101101Solcar 1Add
BLAST
Repeati122 – 20887Solcar 2Add
BLAST
Repeati220 – 30485Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000212132.
InParanoidiQ06497.
OMAiMANIAVY.
OrthoDBiEOG7F2542.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06497-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTLESALTG AVASAMANIA VYPLDLSKTI IQSQVSPSSS EDSNEGKVLP
60 70 80 90 100
NRRYKNVVDC MINIFKEKGI LGLYQGMTVT TVATFVQNFV YFFWYTFIRK
110 120 130 140 150
SYMKHKLLGL QSLKNRDGPI TPSTIEELVL GVAAASISQL FTSPMAVVAT
160 170 180 190 200
RQQTVHSAES AKFTNVIKDI YRENNGDITA FWKGLRTGLA LTINPSITYA
210 220 230 240 250
SFQRLKEVFF HDHSNDAGSL SAVQNFILGV LSKMISTLVT QPLIVAKAML
260 270 280 290 300
QSAGSKFTTF QEALLYLYKN EGLKSLWKGV LPQLTKGVIV QGLLFAFRGE
310 320
LTKSLKRLIF LYSSFFLKHN GQRKLAST
Length:328
Mass (Da):36,367
Last modified:November 1, 1996 - v1
Checksum:iED24312E5F57DBB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40829 Genomic DNA. Translation: AAB68270.1.
BK006949 Genomic DNA. Translation: DAA11541.1.
PIRiS69019.
RefSeqiNP_015453.1. NM_001184225.1.

Genome annotation databases

EnsemblFungiiYPR128C; YPR128C; YPR128C.
GeneIDi856246.
KEGGisce:YPR128C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40829 Genomic DNA. Translation: AAB68270.1.
BK006949 Genomic DNA. Translation: DAA11541.1.
PIRiS69019.
RefSeqiNP_015453.1. NM_001184225.1.

3D structure databases

ProteinModelPortaliQ06497.
SMRiQ06497. Positions 4-288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36295. 39 interactions.
DIPiDIP-8825N.
IntActiQ06497. 2 interactions.
MINTiMINT-4506416.

Protein family/group databases

TCDBi2.A.29.17.1. the mitochondrial carrier (mc) family.

Proteomic databases

MaxQBiQ06497.
PeptideAtlasiQ06497.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR128C; YPR128C; YPR128C.
GeneIDi856246.
KEGGisce:YPR128C.

Organism-specific databases

EuPathDBiFungiDB:YPR128C.
SGDiS000006332. ANT1.

Phylogenomic databases

HOGENOMiHOG000212132.
InParanoidiQ06497.
OMAiMANIAVY.
OrthoDBiEOG7F2542.

Enzyme and pathway databases

BioCyciYEAST:G3O-34265-MONOMER.

Miscellaneous databases

NextBioi981516.
PROiQ06497.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification and functional reconstitution of the yeast peroxisomal adenine nucleotide transporter."
    Palmieri L., Rottensteiner H., Girzalsky W., Scarcia P., Palmieri F., Erdmann R.
    EMBO J. 20:5049-5059(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Identification of a peroxisomal ATP carrier required for medium-chain fatty acid beta-oxidation and normal peroxisome proliferation in Saccharomyces cerevisiae."
    van Roermund C.W.T., Drissen R., van Den Berg M., Ijlst L., Hettema E.H., Tabak H.F., Waterham H.R., Wanders R.J.A.
    Mol. Cell. Biol. 21:4321-4329(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "The peroxisomal transporter gene ANT1 is regulated by a deviant oleate response element (ORE): characterization of the signal for fatty acid induction."
    Rottensteiner H., Palmieri L., Hartig A., Hamilton B., Ruis H., Erdmann R., Gurvitz A.
    Biochem. J. 365:109-117(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "The yeast peroxisomal adenine nucleotide transporter: characterization of two transport modes and involvement in DeltapH formation across peroxisomal membranes."
    Lasorsa F.M., Scarcia P., Erdmann R., Palmieri F., Rottensteiner H., Palmieri L.
    Biochem. J. 381:581-585(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: FUNCTION.

Entry informationi

Entry nameiANT1_YEAST
AccessioniPrimary (citable) accession number: Q06497
Secondary accession number(s): D6W4C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.