Reviewed, UniProtKB/Swiss-Prot Q06497 (ANT1_YEAST)

Names and origin

Protein names

Recommended name:
    Peroxisomal adenine nucleotide transporter 1

Gene names

Name:	ANT1
Ordered Locus Names:	YPR128C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Adenine nucleotide transporter involved in the uniport of ATP and adenine nucleotide hetero-exchange transport between the cytosol and the peroxisomal lumen. This transport is accompanied by a proton transport from the peroxisomal lumen to the cytosol. Transport of ATP into the peroxisome is required for beta-oxydation of medium-chain fatty acids. Required for growth on medium-chain fatty acids, pH gradient formation in peroxisomes and for normal peroxisome proliferation. Ref.2 Ref.3 Ref.7 Ref.8
Subcellular location	Peroxisome membrane; Multi-pass membrane protein.
Induction	Induced by oleic acid, through its ORE-like (oleate responsive element) promoter element and the PIP2-OAF1 transcription factors. Ref.4
Miscellaneous	Present with 2250 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Membrane Peroxisome
Domain	Repeat Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP transport Ref.2 Inferred from direct assay. Source: SGD fatty acid beta-oxidation Ref.2 Ref.3 Inferred from genetic interaction. Source: SGD peroxisome organization Ref.3 Inferred from mutant phenotype. Source: SGD proton transport Inferred from electronic annotation. Source: UniProtKB-KW transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to peroxisomal membrane Ref.2 Inferred from direct assay. Source: SGD
Molecular function	adenine nucleotide transmembrane transporter activity Ref.2 Ref.3 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ECM4	P36156	1	EBI-36879,EBI-2042717
PEX11	Q12462	1	EBI-36879,EBI-13198

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Peroxisomal adenine nucleotide transporter 1		PRO_0000227603
Regions
	Transmembrane	1 – 21	21	1 Potential
	Transmembrane	78 – 98	21	2 Potential
	Transmembrane	128 – 148	21	3 Potential
	Transmembrane	185 – 202	18	4 Potential
	Transmembrane	226 – 246	21	5 Potential
	Transmembrane	277 – 297	21	6 Potential
	Repeat	1 – 101	101	Solcar 1
	Repeat	122 – 208	87	Solcar 2
	Repeat	220 – 304	85	Solcar 3

Sequences

	Sequence		Length	Mass (Da)
	Q06497-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: ED24312E5F57DBB2	FASTA	328	36,367
	10 20 30 40 50 60 MLTLESALTG AVASAMANIA VYPLDLSKTI IQSQVSPSSS EDSNEGKVLP NRRYKNVVDC 70 80 90 100 110 120 MINIFKEKGI LGLYQGMTVT TVATFVQNFV YFFWYTFIRK SYMKHKLLGL QSLKNRDGPI 130 140 150 160 170 180 TPSTIEELVL GVAAASISQL FTSPMAVVAT RQQTVHSAES AKFTNVIKDI YRENNGDITA 190 200 210 220 230 240 FWKGLRTGLA LTINPSITYA SFQRLKEVFF HDHSNDAGSL SAVQNFILGV LSKMISTLVT 250 260 270 280 290 300 QPLIVAKAML QSAGSKFTTF QEALLYLYKN EGLKSLWKGV LPQLTKGVIV QGLLFAFRGE 310 320 LTKSLKRLIF LYSSFFLKHN GQRKLAST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Identification and functional reconstitution of the yeast peroxisomal adenine nucleotide transporter." Palmieri L., Rottensteiner H., Girzalsky W., Scarcia P., Palmieri F., Erdmann R. EMBO J. 20:5049-5059(2001) [PubMed: 11566870] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[3]	"Identification of a peroxisomal ATP carrier required for medium-chain fatty acid beta-oxidation and normal peroxisome proliferation in Saccharomyces cerevisiae." van Roermund C.W.T., Drissen R., van Den Berg M., Ijlst L., Hettema E.H., Tabak H.F., Waterham H.R., Wanders R.J.A. Mol. Cell. Biol. 21:4321-4329(2001) [PubMed: 11390660] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"The peroxisomal transporter gene ANT1 is regulated by a deviant oleate response element (ORE): characterization of the signal for fatty acid induction." Rottensteiner H., Palmieri L., Hartig A., Hamilton B., Ruis H., Erdmann R., Gurvitz A. Biochem. J. 365:109-117(2002) [PubMed: 12071844] [Abstract] Cited for: INDUCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The yeast peroxisomal adenine nucleotide transporter: characterization of two transport modes and involvement in DeltapH formation across peroxisomal membranes." Lasorsa F.M., Scarcia P., Erdmann R., Palmieri F., Rottensteiner H., Palmieri L. Biochem. J. 381:581-585(2004) [PubMed: 15219179] [Abstract] Cited for: FUNCTION.
[8]	"The peroxisomal lumen in Saccharomyces cerevisiae is alkaline." van Roermund C.W.T., de Jong M., Ijlst L., van Marle J., Dansen T.B., Wanders R.J.A., Waterham H.R. J. Cell Sci. 117:4231-4237(2004) [PubMed: 15316083] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U40829 Genomic DNA. Translation: AAB68270.1.
PIR	S69019.
RefSeq	NP_015453.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:8825N.
IntAct	Q06497. 3 interactions.
STRING	Q06497.
Protein family/group databases
TCDB	2.A.29.17.1. mitochondrial carrier (MC) family.
Proteomic databases
PeptideAtlas	Q06497.
Genome annotation databases
Ensembl	YPR128C; YPR128C; YPR128C; Saccharomyces cerevisiae. [Genome view]
GeneID	856246.
KEGG	sce:YPR128C.
NMPDR	fig|4932.3.peg.6590.
Organism-specific databases
CYGD	YPR128c.
SGD	S000006332. ANT1.
Phylogenomic databases
HOGENOM	Q06497.
OMA	MVLCINP
OrthoDB	EOG92V9ZD
Gene expression databases
ArrayExpress	Q06497.
Genevestigator	Q06497.
GermOnline	YPR128C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001993. Mitochondria_substrate_carrier. IPR018108. Mitochondrial_sb/sol_carrier. [Graphical view]
PANTHER	PTHR11896. Mitoch_carrier. 1 hit.
Pfam	PF00153. Mito_carr. 3 hits. [Graphical view]
PROSITE	PS50920. SOLCAR. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981516.

Entry information

Entry name

ANT1_YEAST

Accession

Primary (citable) accession number: Q06497

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)