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Protein

Sodium-dependent phosphate transport protein 2A

Gene

Slc34a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in actively transporting phosphate into cells via Na+ cotransport in the renal brush border membrane. Probably mediates 70-80% of the apical influx.

GO - Molecular functioni

  • PDZ domain binding Source: RGD
  • protein complex binding Source: RGD
  • protein homodimerization activity Source: RGD
  • sodium-dependent phosphate transmembrane transporter activity Source: RGD
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

  • arsenate ion transmembrane transport Source: RGD
  • cellular response to metal ion Source: RGD
  • cellular response to parathyroid hormone stimulus Source: RGD
  • cellular response to staurosporine Source: RGD
  • dentinogenesis Source: RGD
  • gentamycin metabolic process Source: RGD
  • glycoprotein metabolic process Source: RGD
  • indole metabolic process Source: RGD
  • kidney development Source: RGD
  • ossification Source: RGD
  • phosphate ion homeostasis Source: Ensembl
  • phosphate ion transport Source: RGD
  • positive regulation of membrane potential Source: RGD
  • positive regulation of phosphate transmembrane transport Source: RGD
  • positive regulation of sodium-dependent phosphate transport Source: RGD
  • protein homooligomerization Source: RGD
  • response to cadmium ion Source: Ensembl
  • response to drug Source: RGD
  • response to estradiol Source: RGD
  • response to growth hormone Source: RGD
  • response to lead ion Source: Ensembl
  • response to magnesium ion Source: RGD
  • response to mercury ion Source: Ensembl
  • response to nutrient Source: RGD
  • response to parathyroid hormone Source: RGD
  • response to peptide Source: RGD
  • response to peptide hormone Source: RGD
  • response to potassium ion Source: RGD
  • response to thyroid hormone Source: RGD
  • response to vitamin A Source: RGD
  • sodium-dependent phosphate transport Source: RGD
  • sodium ion transport Source: UniProtKB-KW
  • tricarboxylic acid metabolic process Source: RGD
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-RNO-427589. Type II Na+/Pi cotransporters.
R-RNO-5683826. Surfactant metabolism.

Protein family/group databases

TCDBi2.A.58.1.1. the phosphate:na(+) symporter (pnas) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent phosphate transport protein 2A
Short name:
Sodium-phosphate transport protein 2A
Alternative name(s):
Na(+)-dependent phosphate cotransporter 2A
Sodium/phosphate cotransporter 2A
Short name:
Na(+)/Pi cotransporter 2A
Short name:
NaPi-2a
Solute carrier family 34 member 1
Gene namesi
Name:Slc34a1
Synonyms:Slc17a2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi3708. Slc34a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 103103CytoplasmicSequence analysisAdd
BLAST
Transmembranei104 – 12522Helical; Name=M1Sequence analysisAdd
BLAST
Topological domaini126 – 14520ExtracellularSequence analysisAdd
BLAST
Transmembranei146 – 16318Helical; Name=M2Sequence analysisAdd
BLAST
Topological domaini164 – 21653CytoplasmicSequence analysisAdd
BLAST
Transmembranei217 – 23620Helical; Name=M3Sequence analysisAdd
BLAST
Topological domaini237 – 345109ExtracellularSequence analysisAdd
BLAST
Transmembranei346 – 36823Helical; Name=M4Sequence analysisAdd
BLAST
Topological domaini369 – 41042CytoplasmicSequence analysisAdd
BLAST
Transmembranei411 – 43424Helical; Name=M5Sequence analysisAdd
BLAST
Topological domaini435 – 46430ExtracellularSequence analysisAdd
BLAST
Transmembranei465 – 48521Helical; Name=M6Sequence analysisAdd
BLAST
Topological domaini486 – 51126CytoplasmicSequence analysisAdd
BLAST
Transmembranei512 – 53221Helical; Name=M7Sequence analysisAdd
BLAST
Topological domaini533 – 5375ExtracellularSequence analysis
Transmembranei538 – 55922Helical; Name=M8Sequence analysisAdd
BLAST
Topological domaini560 – 63778CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • brush border membrane Source: RGD
  • cell surface Source: RGD
  • endosome Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • intrinsic component of plasma membrane Source: RGD
  • membrane raft Source: RGD
  • perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637Sodium-dependent phosphate transport protein 2APRO_0000068610Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineCombined sources
Modified residuei34 – 341PhosphoserineBy similarity
Disulfide bondi225 ↔ 520Sequence analysis
Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence analysis
Disulfide bondi306 ↔ 3341 Publication
Glycosylationi328 – 3281N-linked (GlcNAc...)Sequence analysis
Modified residuei506 – 5061Phosphothreonine; by PKCSequence analysis
Modified residuei605 – 6051PhosphoserineCombined sources
Modified residuei621 – 6211PhosphothreonineCombined sources
Modified residuei623 – 6231PhosphoserineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ06496.
PRIDEiQ06496.

PTM databases

iPTMnetiQ06496.

Expressioni

Tissue specificityi

Kidney.

Inductioni

By low-phosphate diet.1 Publication

Gene expression databases

GenevisibleiQ06496. RN.

Interactioni

Subunit structurei

Interacts via its C-terminal region with PDZK2. Interacts with SLC9A3R1.By similarity

GO - Molecular functioni

  • PDZ domain binding Source: RGD
  • protein complex binding Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031764.

Family & Domainsi

Sequence similaritiesi

Belongs to the SLC34A transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IE8P. Eukaryota.
COG1283. LUCA.
GeneTreeiENSGT00390000005032.
HOGENOMiHOG000006550.
HOVERGENiHBG006527.
InParanoidiQ06496.
KOiK14683.
OMAiCPYGEVL.
OrthoDBiEOG72ZCDP.
PhylomeDBiQ06496.
TreeFamiTF313981.

Family and domain databases

InterProiIPR003841. Na/Pi_transpt.
IPR029848. Na/Pi_transpt_2A.
[Graphical view]
PANTHERiPTHR10010:SF21. PTHR10010:SF21. 1 hit.
PfamiPF02690. Na_Pi_cotrans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01013. 2a58. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06496-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSYSERLGG PAVSPLPVRG RHMVHGAAFA YVPSPQVLHR IPGTTTYAIS
60 70 80 90 100
SLSPVALTEH SCPYGEVLEC HDPLPAKLAQ EEEQKPEPRL SQKLAQVGTK
110 120 130 140 150
LLKVPLMLGF LYLFVCSLDV LSSAFQLAGG KVAGDIFKDN AILSNPVAGL
160 170 180 190 200
VVGILVTVLV QSSSTSTSII VSMVSSGLLE VSSAIPIIMG SNIGTSVTNT
210 220 230 240 250
IVALMQAGDR TDFRRAFAGA TVHDCFNWLS VLVLLPLEAA TGYLHHVTGL
260 270 280 290 300
VVASFNIRGG RDAPDLLKVI TEPFTKLIIQ LDKSVITSIA VGDESLRNHS
310 320 330 340 350
LIRIWCQPET KEASTSMSRV EAIGSLANTT MEKCNHIFVD TGLPDLAVGL
360 370 380 390 400
ILLAGSLVVL CTCLILLVKM LNSLLKGQVA NVIQKVINTD FPAPFTWVTG
410 420 430 440 450
YFAMVVGASM TFVVQSSSVF TSAITPLIGL GVISIERAYP LTLGSNIGTT
460 470 480 490 500
TTAILAALAS PREKLSSSFQ IALCHFFFNI SGILLWYPLP CTRLPIRMAK
510 520 530 540 550
ALGKRTAKYR WFAVLYLLVC FLLLPSLVFG ISMAGWQAMV GVGTPFGALL
560 570 580 590 600
AFVVLVNVLQ SRSPGHLPKW LQTWDFLPRW MHSLQPLDGL ITRATLCYAR
610 620 630
PEPRSPQLPP RVFLEELPPA TPSPRLALPA HHNATRL
Length:637
Mass (Da):68,707
Last modified:February 1, 1995 - v1
Checksum:i34D02E7817683F42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13257 mRNA. Translation: AAC37608.1.
BC078876 mRNA. Translation: AAH78876.1.
PIRiA48189.
RefSeqiNP_037162.1. NM_013030.1.
UniGeneiRn.92022.

Genome annotation databases

EnsembliENSRNOT00000033749; ENSRNOP00000031764; ENSRNOG00000015262.
GeneIDi25548.
KEGGirno:25548.
UCSCiRGD:3708. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13257 mRNA. Translation: AAC37608.1.
BC078876 mRNA. Translation: AAH78876.1.
PIRiA48189.
RefSeqiNP_037162.1. NM_013030.1.
UniGeneiRn.92022.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031764.

Protein family/group databases

TCDBi2.A.58.1.1. the phosphate:na(+) symporter (pnas) family.

PTM databases

iPTMnetiQ06496.

Proteomic databases

PaxDbiQ06496.
PRIDEiQ06496.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000033749; ENSRNOP00000031764; ENSRNOG00000015262.
GeneIDi25548.
KEGGirno:25548.
UCSCiRGD:3708. rat.

Organism-specific databases

CTDi6569.
RGDi3708. Slc34a1.

Phylogenomic databases

eggNOGiENOG410IE8P. Eukaryota.
COG1283. LUCA.
GeneTreeiENSGT00390000005032.
HOGENOMiHOG000006550.
HOVERGENiHBG006527.
InParanoidiQ06496.
KOiK14683.
OMAiCPYGEVL.
OrthoDBiEOG72ZCDP.
PhylomeDBiQ06496.
TreeFamiTF313981.

Enzyme and pathway databases

ReactomeiR-RNO-427589. Type II Na+/Pi cotransporters.
R-RNO-5683826. Surfactant metabolism.

Miscellaneous databases

PROiQ06496.

Gene expression databases

GenevisibleiQ06496. RN.

Family and domain databases

InterProiIPR003841. Na/Pi_transpt.
IPR029848. Na/Pi_transpt_2A.
[Graphical view]
PANTHERiPTHR10010:SF21. PTHR10010:SF21. 1 hit.
PfamiPF02690. Na_Pi_cotrans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01013. 2a58. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. "Regulation of rat renal Na/Pi-cotransporter by parathyroid hormone: immunohistochemistry."
    Lotscher M., Kaissling B., Biber J., Murer H., Kempson S.A., Levi M.
    Kidney Int. 49:1010-1011(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION BY PTH.
  4. "Acute regulation by dietary phosphate of the sodium-dependent phosphate transporter (NaP(i)-2) in rat kidney."
    Katai K., Segawa H., Haga H., Morita K., Arai H., Tatsumi S., Taketani Y., Miyamoto K., Hisano S., Fukui Y., Takeda E.
    J. Biochem. 121:50-55(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "Parathyroid hormone leads to the lysosomal degradation of the renal type II Na/Pi cotransporter."
    Pfister M.F., Ruf I., Stange G., Ziegler U., Lederer E., Biber J., Murer H.
    Proc. Natl. Acad. Sci. U.S.A. 95:1909-1914(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION BY PTH.
  6. "Stoichiometry and Na+ binding cooperativity of rat and flounder renal type II Na+-Pi cotransporters."
    Forster I.C., Loo D.D., Eskandari S.
    Am. J. Physiol. 276:F644-F649(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: STOICHIOMETRY.
  7. "Studies on the topology of the renal type II NaPi-cotransporter."
    Lambert G., Traebert M., Hernando N., Biber J., Murer H.
    Pflugers Arch. 437:972-978(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  8. "Cysteine residues and the structure of the rat renal proximal tubular type II sodium phosphate cotransporter (rat NaPi IIa)."
    Lambert G., Forster I.C., Biber J., Murer H.
    J. Membr. Biol. 176:133-141(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS, REVISED TOPOLOGY.
  9. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14; SER-605; THR-621 AND SER-623, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNPT2A_RAT
AccessioniPrimary (citable) accession number: Q06496
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The cotransport has a Na+:Pi stoichiometry of 3:1 and is electrogenic.
PTH induces lysosomal degradation of the cotransporter.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.