Reviewed, UniProtKB/Swiss-Prot Q06494 (PLR1_YEAST)

Names and origin

Protein names

Recommended name:
    Putative pyridoxal reductase
      Short name=PL reductase
      Short name=PL-red
    EC=1.1.1.65

Gene names

Ordered Locus Names:

YPR127W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reduction of pyridoxal (PL) with NADPH and oxidation of pyridoxine (PN) with NADP(+) By similarity.
Catalytic activity	Pyridoxine + NADP(+) = pyridoxal + NADPH.
Pathway	Cofactor degradation; B6 vitamer degradation; pyridoxal from pyridoxine (dehydrogenase route): step 1/1.
Subcellular location	Cytoplasm. Nucleus.
Miscellaneous	Present with 2190 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Ref.2 Inferred from electronic annotation. Source: UniProtKB-KW nucleus Ref.2 Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	pyridoxine 4-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Putative pyridoxal reductase		PRO_0000257816
Sites
	Active site	60	1	Proton donor By similarity
Amino acid modifications
	Modified residue	87	1	Phosphoserine
	Modified residue	98	1	Phosphothreonine

Sequences

	Sequence		Length	Mass (Da)
	Q06494-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C443ED79F25EA106	FASTA	345	38,601
	10 20 30 40 50 60 MSVADLKNNI HKLDTGYGLM SLTWRAEPIP QSQAFEAMHR VVELSRERGH KAFFNVGEFY 70 80 90 100 110 120 GPDFINLSYV HDFFAKYPDL RKDVVISCKG GADNATLTPR GSHDDVVQSV KNSVSAIGGY 130 140 150 160 170 180 IDIFEVARID TSLCTKGEVY PYESFEALAE MISEGVIGGI SLSEVNEEQI RAIHKDWGKF 190 200 210 220 230 240 LTCVEVELSL FSNDILHNGI AKTCAELGLS IICYSPLGRG LLTGQLKSNA DIPEGDFRKS 250 260 270 280 290 300 LKRFSDESLK KNLTLVRFLQ EEIVDKRPQN NSITLAQLAL GWVKHWNKVP EYSGAKFIPI 310 320 330 340 PSGSSISKVN ENFDEQKTKL TDQEFNAINK YLTTFHTVGD RYEMA

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND THR-98, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U40828 Genomic DNA. Translation: AAB68066.1.
PIR	S69018.
RefSeq	NP_015452.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q06494.
Proteomic databases
PeptideAtlas	Q06494.
Genome annotation databases
Ensembl	YPR127W. Saccharomyces cerevisiae. [Contig view]
GeneID	856245.
GenomeReviews	Gene locus YPR127W in contig U00094_GR.
KEGG	sce:YPR127W.
NMPDR	fig|4932.3.peg.6589.
Organism-specific databases
CYGD	YPR127w.
SGD	S000006331. YPR127W.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q06494.
Gene expression databases
GermOnline	YPR127W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. False negative. PS00062. ALDOKETO_REDUCTASE_2. False negative. PS00063. ALDOKETO_REDUCTASE_3. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q06494.
NextBio	981513.

Entry information

Entry name

PLR1_YEAST

Accession

Primary (citable) accession number: Q06494

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)