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Q06489 (MTNA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylthioribose-1-phosphate isomerase
Short name=M1Pi
Short name=MTR-1-P isomerase
EC=5.3.1.23
Alternative name(s):
S-methyl-5-thioribose-1-phosphate isomerase
Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein
Gene names
Name:MRI1
Ordered Locus Names:YPR118W
ORF Names:P9642.7
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length411 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P). Ref.6 Ref.7

Catalytic activity

S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate.

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6.

Subunit structure

Homodimer. Ref.7

Subcellular location

Cytoplasm. Nucleus Ref.3.

Miscellaneous

Present with 922 molecules/cell in log phase SD medium. Ref.4

Sequence similarities

Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 411411Methylthioribose-1-phosphate isomerase
PRO_0000156110

Sites

Active site2801Proton donor By similarity
Site1811Transition state stabilizer By similarity

Amino acid modifications

Modified residue3511Phosphoserine Ref.5

Secondary structure

1 ....................................................................... 411
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q06489 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 369B2114E12E11D8

FASTA41145,020
        10         20         30         40         50         60 
MSLEAIVFDR SEPENVSVKV LDQLLLPYTT KYVPIHTIDD GYSVIKSMQV RGAPAIAIVG 

        70         80         90        100        110        120 
SLSVLTEVQL IKHNPTSDVA TLYSLVNWES TKTVLNKRLD FLLSSRPTAV NLSNSLVEIK 

       130        140        150        160        170        180 
NILKSSSDLK AFDGSLYNYV CELIDEDLAN NMKMGDNGAK YLIDVLQKDG FKDEFAVLTI 

       190        200        210        220        230        240 
CNTGSLATSG YGTALGVIRS LWKDSLAKTD KADSGLDNEK CPRMGHVFPL ETRPYNQGSR 

       250        260        270        280        290        300 
LTAYELVYDK IPSTLITDSS IAYRIRTSPI PIKAAFVGAD RIVRNGDTAN KIGTLQLAVI 

       310        320        330        340        350        360 
CKQFGIKFFV VAPKTTIDNV TETGDDIIVE ERNPEEFKVV TGTVINPENG SLILNESGEP 

       370        380        390        400        410 
ITGKVGIAPL EINVWNPAFD ITPHELIDGI ITEEGVFTKN SSGEFQLESL F

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed: 9169875] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351, MASS SPECTROMETRY.
[6]"A complete inventory of all enzymes in the eukaryotic methionine salvage pathway."
Pirkov I., Norbeck J., Gustafsson L., Albers E.
FEBS J. 275:4111-4120(2008) [PubMed: 18625006] [Abstract]
Cited for: FUNCTION.
[7]"Crystal structure of yeast Ypr118w, a methylthioribose-1-phosphate isomerase related to regulatory eIF2B subunits."
Bumann M., Djafarzadeh S., Oberholzer A.E., Bigler P., Altmann M., Trachsel H., Baumann U.
J. Biol. Chem. 279:37087-37094(2004) [PubMed: 15215245] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS), SUBUNIT, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U40828 Genomic DNA. Translation: AAB68059.1.
BK006949 Genomic DNA. Translation: DAA11533.1.
PIRS69011.
RefSeqNP_015443.1. NM_001184215.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1W2WX-ray1.75A/E/I/M1-211[»]
B/F/J/N221-411[»]
ProteinModelPortalQ06489.
SMRQ06489. Positions 1-211, 221-411.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4685N.
IntActQ06489. 1 interaction.
MINTMINT-505844.
STRINGQ06489.

Proteomic databases

PeptideAtlasQ06489.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR118W; YPR118W; YPR118W.
GeneID856234.
KEGGsce:YPR118W.
NMPDRfig|4932.3.peg.6580.

Organism-specific databases

CYGDYPR118w.
SGDS000006322. MRI1.

Phylogenomic databases

eggNOGfuNOG06631.
GeneTreeEFGT00050000003245.
HOGENOMHBG682649.
OMAEDGWKVI.
OrthoDBEOG4S1XGM.

Gene expression databases

ArrayExpressQ06489.
GenevestigatorQ06489.
GermOnlineYPR118W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000649. IF-2B-related.
IPR005251. IF-2BI_MTNA.
IPR011559. Initiation_fac_2B_a/b/d.
[Graphical view]
KOK08963.
PANTHERPTHR10233. IF-2B_related. 1 hit.
PTHR10233:SF6. PTHR10233:SF6. 1 hit.
PfamPF01008. IF-2B. 1 hit.
[Graphical view]
TIGRFAMsTIGR00524. EIF-2B_rel. 1 hit.
TIGR00512. Salvage_mtnA. 1 hit.
ProtoNetSearch...

Other

NextBio981488.

Entry information

Entry nameMTNA_YEAST
AccessionPrimary (citable) accession number: Q06489
Secondary accession number(s): D6W4B7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: December 14, 2011
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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