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Protein

Methylthioribose-1-phosphate isomerase

Gene

MRI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).UniRule annotation2 Publications

Catalytic activityi

S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (MRI1)
  2. Methylthioribulose-1-phosphate dehydratase (MDE1)
  3. Enolase-phosphatase E1 (UTR4)
  4. Enolase-phosphatase E1 (UTR4)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)
  6. Aromatic amino acid aminotransferase 2 (ARO9), Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2), Aromatic/aminoadipate aminotransferase 1 (ARO8)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei181Transition state stabilizerUniRule annotation1
Active sitei280Proton donorUniRule annotation1

GO - Molecular functioni

  • S-methyl-5-thioribose-1-phosphate isomerase activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-34257-MONOMER.
ReactomeiR-SCE-1237112. Methionine salvage pathway.
UniPathwayiUPA00904; UER00874.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylthioribose-1-phosphate isomeraseUniRule annotation (EC:5.3.1.23UniRule annotation)
Short name:
M1PiUniRule annotation
Short name:
MTR-1-P isomeraseUniRule annotation
Alternative name(s):
S-methyl-5-thioribose-1-phosphate isomeraseUniRule annotation
Translation initiation factor eIF-2B subunit alpha/beta/delta-like proteinUniRule annotation
Gene namesi
Name:MRI1UniRule annotation
Ordered Locus Names:YPR118W
ORF Names:P9642.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR118W.
SGDiS000006322. MRI1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001561102 – 411Methylthioribose-1-phosphate isomeraseAdd BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei351PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ06489.
PRIDEiQ06489.

PTM databases

iPTMnetiQ06489.

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi36285. 25 interactors.
DIPiDIP-4685N.
IntActiQ06489. 1 interactor.
MINTiMINT-505844.

Structurei

Secondary structure

1411
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Beta strandi17 – 21Combined sources5
Turni23 – 28Combined sources6
Beta strandi32 – 34Combined sources3
Helixi38 – 46Combined sources9
Helixi53 – 73Combined sources21
Helixi79 – 82Combined sources4
Helixi88 – 103Combined sources16
Helixi111 – 124Combined sources14
Helixi129 – 168Combined sources40
Beta strandi173 – 179Combined sources7
Helixi185 – 187Combined sources3
Beta strandi188 – 191Combined sources4
Helixi194 – 210Combined sources17
Beta strandi223 – 230Combined sources8
Turni233 – 236Combined sources4
Helixi237 – 240Combined sources4
Helixi242 – 249Combined sources8
Beta strandi253 – 256Combined sources4
Helixi258 – 260Combined sources3
Helixi261 – 267Combined sources7
Beta strandi272 – 277Combined sources6
Beta strandi280 – 282Combined sources3
Beta strandi288 – 291Combined sources4
Helixi294 – 304Combined sources11
Beta strandi307 – 311Combined sources5
Helixi314 – 316Combined sources3
Helixi324 – 326Combined sources3
Helixi335 – 338Combined sources4
Beta strandi339 – 345Combined sources7
Turni347 – 349Combined sources3
Beta strandi362 – 366Combined sources5
Beta strandi377 – 382Combined sources6
Helixi384 – 386Combined sources3
Beta strandi388 – 392Combined sources5
Beta strandi395 – 397Combined sources3
Helixi408 – 410Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1W2WX-ray1.75A/E/I/M1-211[»]
B/F/J/N221-411[»]
ProteinModelPortaliQ06489.
SMRiQ06489.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06489.

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000013732.
HOGENOMiHOG000224730.
InParanoidiQ06489.
KOiK08963.
OMAiHIYACET.
OrthoDBiEOG092C3FYS.

Family and domain databases

Gene3Di1.20.120.420. 1 hit.
HAMAPiMF_01678. Salvage_MtnA. 1 hit.
InterProiIPR000649. IF-2B-related.
IPR005251. IF-M1Pi.
IPR011559. Initiation_fac_2B_a/b/d.
IPR027363. M1Pi_N.
[Graphical view]
PfamiPF01008. IF-2B. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00524. eIF-2B_rel. 1 hit.
TIGR00512. salvage_mtnA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06489-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLEAIVFDR SEPENVSVKV LDQLLLPYTT KYVPIHTIDD GYSVIKSMQV
60 70 80 90 100
RGAPAIAIVG SLSVLTEVQL IKHNPTSDVA TLYSLVNWES TKTVLNKRLD
110 120 130 140 150
FLLSSRPTAV NLSNSLVEIK NILKSSSDLK AFDGSLYNYV CELIDEDLAN
160 170 180 190 200
NMKMGDNGAK YLIDVLQKDG FKDEFAVLTI CNTGSLATSG YGTALGVIRS
210 220 230 240 250
LWKDSLAKTD KADSGLDNEK CPRMGHVFPL ETRPYNQGSR LTAYELVYDK
260 270 280 290 300
IPSTLITDSS IAYRIRTSPI PIKAAFVGAD RIVRNGDTAN KIGTLQLAVI
310 320 330 340 350
CKQFGIKFFV VAPKTTIDNV TETGDDIIVE ERNPEEFKVV TGTVINPENG
360 370 380 390 400
SLILNESGEP ITGKVGIAPL EINVWNPAFD ITPHELIDGI ITEEGVFTKN
410
SSGEFQLESL F
Length:411
Mass (Da):45,020
Last modified:November 1, 1996 - v1
Checksum:i369B2114E12E11D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40828 Genomic DNA. Translation: AAB68059.1.
BK006949 Genomic DNA. Translation: DAA11533.1.
PIRiS69011.
RefSeqiNP_015443.1. NM_001184215.1.

Genome annotation databases

EnsemblFungiiYPR118W; YPR118W; YPR118W.
GeneIDi856234.
KEGGisce:YPR118W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40828 Genomic DNA. Translation: AAB68059.1.
BK006949 Genomic DNA. Translation: DAA11533.1.
PIRiS69011.
RefSeqiNP_015443.1. NM_001184215.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1W2WX-ray1.75A/E/I/M1-211[»]
B/F/J/N221-411[»]
ProteinModelPortaliQ06489.
SMRiQ06489.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36285. 25 interactors.
DIPiDIP-4685N.
IntActiQ06489. 1 interactor.
MINTiMINT-505844.

PTM databases

iPTMnetiQ06489.

Proteomic databases

MaxQBiQ06489.
PRIDEiQ06489.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR118W; YPR118W; YPR118W.
GeneIDi856234.
KEGGisce:YPR118W.

Organism-specific databases

EuPathDBiFungiDB:YPR118W.
SGDiS000006322. MRI1.

Phylogenomic databases

GeneTreeiENSGT00390000013732.
HOGENOMiHOG000224730.
InParanoidiQ06489.
KOiK08963.
OMAiHIYACET.
OrthoDBiEOG092C3FYS.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00874.
BioCyciYEAST:G3O-34257-MONOMER.
ReactomeiR-SCE-1237112. Methionine salvage pathway.

Miscellaneous databases

EvolutionaryTraceiQ06489.
PROiQ06489.

Family and domain databases

Gene3Di1.20.120.420. 1 hit.
HAMAPiMF_01678. Salvage_MtnA. 1 hit.
InterProiIPR000649. IF-2B-related.
IPR005251. IF-M1Pi.
IPR011559. Initiation_fac_2B_a/b/d.
IPR027363. M1Pi_N.
[Graphical view]
PfamiPF01008. IF-2B. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00524. eIF-2B_rel. 1 hit.
TIGR00512. salvage_mtnA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTNA_YEAST
AccessioniPrimary (citable) accession number: Q06489
Secondary accession number(s): D6W4B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 922 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.