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Protein

Chromatin structure-remodeling complex subunit RSC2

Gene

RSC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is involved in meiotic sporulation through regulating IME2 expression, and is also essential for 2-micron plasmid maintenance and for normal REP1 protein localization.8 Publications

GO - Molecular functioni

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: UniProtKB
  • chromosome segregation Source: SGD
  • double-strand break repair via homologous recombination Source: SGD
  • double-strand break repair via nonhomologous end joining Source: SGD
  • nucleosome disassembly Source: SGD
  • plasmid maintenance Source: UniProtKB
  • regulation of sporulation resulting in formation of a cellular spore Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • sister chromatid cohesion Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  • transcription elongation from RNA polymerase II promoter Source: SGD
  • UV-damage excision repair Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Sporulation, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32429-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin structure-remodeling complex subunit RSC2
Alternative name(s):
RSC complex subunit RSC2
Remodel the structure of chromatin complex subunit 2
Gene namesi
Name:RSC2
Ordered Locus Names:YLR357W
ORF Names:L9638.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR357W.
SGDiS000004349. RSC2.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.

GO - Cellular componenti

  • RSC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi468 – 4681E → K in dpm3; defective in plasmid maintenance. 1 Publication
Mutagenesisi601 – 6011G → E in dpm18; defective in plasmid maintenance. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 889889Chromatin structure-remodeling complex subunit RSC2PRO_0000211212Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei612 – 6121PhosphotyrosineCombined sources
Modified residuei682 – 6821PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06488.

PTM databases

iPTMnetiQ06488.

Interactioni

Subunit structurei

Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin.

Protein-protein interaction databases

BioGridi31619. 250 interactions.
DIPiDIP-863N.
IntActiQ06488. 53 interactions.
MINTiMINT-666605.

Structurei

Secondary structure

1
889
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi403 – 4053Combined sources
Beta strandi408 – 4103Combined sources
Beta strandi415 – 4184Combined sources
Beta strandi428 – 43710Combined sources
Beta strandi443 – 4519Combined sources
Helixi453 – 4553Combined sources
Beta strandi462 – 4654Combined sources
Beta strandi470 – 47910Combined sources
Helixi480 – 4823Combined sources
Beta strandi483 – 4864Combined sources
Beta strandi488 – 4914Combined sources
Helixi492 – 4976Combined sources
Beta strandi498 – 5014Combined sources
Beta strandi508 – 5114Combined sources
Beta strandi513 – 5164Combined sources
Turni517 – 5204Combined sources
Beta strandi521 – 5244Combined sources
Helixi528 – 5314Combined sources
Helixi534 – 5363Combined sources
Beta strandi543 – 5453Combined sources
Beta strandi547 – 5559Combined sources
Helixi559 – 5613Combined sources
Beta strandi621 – 6266Combined sources
Turni627 – 6304Combined sources
Beta strandi631 – 6333Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BB7X-ray2.40A/B/C/D401-641[»]
ProteinModelPortaliQ06488.
SMRiQ06488. Positions 21-114, 400-633.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 10369Bromo 1PROSITE-ProRule annotationAdd
BLAST
Domaini295 – 36571Bromo 2PROSITE-ProRule annotationAdd
BLAST
Domaini408 – 526119BAHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi667 – 71650Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the RSC1 family.Curated
Contains 1 BAH domain.PROSITE-ProRule annotation
Contains 2 bromo domains.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Repeat

Phylogenomic databases

GeneTreeiENSGT00390000003017.
HOGENOMiHOG000247903.
InParanoidiQ06488.
KOiK11756.
OMAiWFICEFR.
OrthoDBiEOG71RXT3.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001025. BAH_dom.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
[Graphical view]
PfamiPF01426. BAH. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00439. BAH. 1 hit.
SM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS51038. BAH. 1 hit.
PS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMPDDNSNSS TQNSSALYKD LRKEYESLFT LKEDSGLEIS PIFNVLPPKK
60 70 80 90 100
DYPDYYAVIK NPVSFNTLKK RIPHYTDAQQ FMNDVVQIPW NAKTYNTRDS
110 120 130 140 150
GIYKYALVLE KYLKDTIYPN LKEKYPQLVY PDLGPLPDEP GYEEFQQKLR
160 170 180 190 200
EKAEEVARAN AARAESSSSM NSTEAARRLR KTRTSVKRES EPGTDTNNDE
210 220 230 240 250
DYEATDMDID NPKDADFPDL IRKPLININP YTRKPLRDNR STTPSHSGTP
260 270 280 290 300
QPLGPRHRQV SRTQVKRGRP PIIDLPYIQR MKNVMKVLKK EVLDSGIGLT
310 320 330 340 350
DLFERLPDRH RDANYYIMIA NPISLQDINK KVKTRRYKTF QEFQNDFNLM
360 370 380 390 400
LTNFRISHRG DPESIKISNI LEKTFTSLAR FELSKPDRSF IPEGELRYPL
410 420 430 440 450
DEVIVNNISY HVGDWALLRN QNDPQKPIVG QIFRLWKTPD GKQWLNACWY
460 470 480 490 500
YRPEQTVHRV DRLFYKNEVM KTGQYRDHLV SNLVGKCYVI HFTRYQRGNP
510 520 530 540 550
DMKLEGPLFV CEFRYNESDK IFNKIRTWKA CLPEEIRDLD EATIPVNGRK
560 570 580 590 600
FFKYPSPIRH LLPANATPHD RVPEPTMGSP DAPPLVGAVY MRPKMQRDDL
610 620 630 640 650
GEYATSDDCP RYIIRPNDSP EEGQVDIETG TITTNTPTAN ALPKTGYSSS
660 670 680 690 700
KLSSLRYNRS SMSLENQNAI GQQQIPLSRV GSPGAGGPLT VQGLKQHQLQ
710 720 730 740 750
RLQQQQHQYQ QQKRSQASRY NIPTIIDDLT SQASRGNLGN IMIDAASSFV
760 770 780 790 800
LPISITKNVD VLQRTDLHSQ TKRSGREEMF PWKKTKGEIL WFRGPSVIVN
810 820 830 840 850
ERIINSGDPH LSLPLNRWFT TNKKRKLEYE EVEETMEDVT GKDKDDDGLE
860 870 880
PDVENEKESL PGPFVLGLRP SAKFTAHRLS MLRPPSSSS
Length:889
Mass (Da):102,300
Last modified:November 1, 1996 - v1
Checksum:i7867866FD973F188
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19102 Genomic DNA. Translation: AAB67747.1.
BK006945 Genomic DNA. Translation: DAA09661.1.
PIRiS51465.
RefSeqiNP_013461.1. NM_001182246.1.

Genome annotation databases

EnsemblFungiiYLR357W; YLR357W; YLR357W.
GeneIDi851071.
KEGGisce:YLR357W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19102 Genomic DNA. Translation: AAB67747.1.
BK006945 Genomic DNA. Translation: DAA09661.1.
PIRiS51465.
RefSeqiNP_013461.1. NM_001182246.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BB7X-ray2.40A/B/C/D401-641[»]
ProteinModelPortaliQ06488.
SMRiQ06488. Positions 21-114, 400-633.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31619. 250 interactions.
DIPiDIP-863N.
IntActiQ06488. 53 interactions.
MINTiMINT-666605.

PTM databases

iPTMnetiQ06488.

Proteomic databases

MaxQBiQ06488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR357W; YLR357W; YLR357W.
GeneIDi851071.
KEGGisce:YLR357W.

Organism-specific databases

EuPathDBiFungiDB:YLR357W.
SGDiS000004349. RSC2.

Phylogenomic databases

GeneTreeiENSGT00390000003017.
HOGENOMiHOG000247903.
InParanoidiQ06488.
KOiK11756.
OMAiWFICEFR.
OrthoDBiEOG71RXT3.

Enzyme and pathway databases

BioCyciYEAST:G3O-32429-MONOMER.

Miscellaneous databases

PROiQ06488.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001025. BAH_dom.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
[Graphical view]
PfamiPF01426. BAH. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00439. BAH. 1 hit.
SM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS51038. BAH. 1 hit.
PS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "RSC2, encoding a component of the RSC nucleosome remodeling complex, is essential for 2 micrometer plasmid maintenance in Saccharomyces cerevisiae."
    Wong M.C.V.L., Scott-Drew S.R.S., Hayes M.J., Howard P.J., Murray J.A.H.
    Mol. Cell. Biol. 22:4218-4229(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF GLU-468 AND GLY-601.
  4. "Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains."
    Cairns B.R., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg R.D., Winston F.
    Mol. Cell 4:715-723(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 241-253, COMPOSITION OF THE RSC COMPLEX.
  5. Cited for: FUNCTION OF THE RSC COMPLEX, COMPOSITION OF THE RSC COMPLEX.
  6. "Histone octamer transfer by a chromatin-remodeling complex."
    Lorch Y., Zhang M., Kornberg R.D.
    Cell 96:389-392(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  7. "Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC."
    Moreira J.M.A., Holmberg S.
    EMBO J. 18:2836-2844(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  8. "Functional differences between RSC1 and RSC2, components of a for growth essential chromatin-remodeling complex of Saccharomyces cerevisiae, during the sporulation process."
    Yukawa M., Koyama H., Miyahara K., Tsuchiya E.
    FEMS Yeast Res. 2:87-91(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Chromatin remodeling by RSC involves ATP-dependent DNA translocation."
    Saha A., Wittmeyer J., Cairns B.R.
    Genes Dev. 16:2120-2134(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  10. "Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway."
    Chai B., Hsu J.-M., Du J., Laurent B.C.
    Genetics 161:575-584(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  11. "The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation."
    Hsu J.-M., Huang J., Meluh P.B., Laurent B.C.
    Mol. Cell. Biol. 23:3202-3215(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX, SUBCELLULAR LOCATION, INTERACTION OF THE RSC COMPLEX WITH HISTONES.
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  13. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-682, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  14. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-682, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  15. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-682, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-612 AND SER-682, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRSC2_YEAST
AccessioniPrimary (citable) accession number: Q06488
Secondary accession number(s): D6VYZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2330 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.