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Protein

Chromatin structure-remodeling complex subunit RSC2

Gene

RSC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is involved in meiotic sporulation through regulating IME2 expression, and is also essential for 2-micron plasmid maintenance and for normal REP1 protein localization.8 Publications

GO - Molecular functioni

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: UniProtKB
  • chromatin modification Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • double-strand break repair via homologous recombination Source: SGD
  • double-strand break repair via nonhomologous end joining Source: SGD
  • nucleosome disassembly Source: SGD
  • plasmid maintenance Source: UniProtKB
  • regulation of sporulation resulting in formation of a cellular spore Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • sister chromatid cohesion Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  • transcription elongation from RNA polymerase II promoter Source: SGD
  • UV-damage excision repair Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Sporulation, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32429-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin structure-remodeling complex subunit RSC2
Alternative name(s):
RSC complex subunit RSC2
Remodel the structure of chromatin complex subunit 2
Gene namesi
Name:RSC2
Ordered Locus Names:YLR357W
ORF Names:L9638.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR357W.
SGDiS000004349. RSC2.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.

GO - Cellular componenti

  • RSC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi468E → K in dpm3; defective in plasmid maintenance. 1 Publication1
Mutagenesisi601G → E in dpm18; defective in plasmid maintenance. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002112121 – 889Chromatin structure-remodeling complex subunit RSC2Add BLAST889

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei612PhosphotyrosineCombined sources1
Modified residuei682PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06488.
PRIDEiQ06488.

PTM databases

iPTMnetiQ06488.

Interactioni

Subunit structurei

Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin.

Protein-protein interaction databases

BioGridi31619. 250 interactors.
DIPiDIP-863N.
IntActiQ06488. 53 interactors.
MINTiMINT-666605.

Structurei

Secondary structure

1889
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi403 – 405Combined sources3
Beta strandi408 – 410Combined sources3
Beta strandi415 – 418Combined sources4
Beta strandi428 – 437Combined sources10
Beta strandi443 – 451Combined sources9
Helixi453 – 455Combined sources3
Beta strandi462 – 465Combined sources4
Beta strandi470 – 479Combined sources10
Helixi480 – 482Combined sources3
Beta strandi483 – 486Combined sources4
Beta strandi488 – 491Combined sources4
Helixi492 – 497Combined sources6
Beta strandi498 – 501Combined sources4
Beta strandi508 – 511Combined sources4
Beta strandi513 – 516Combined sources4
Turni517 – 520Combined sources4
Beta strandi521 – 524Combined sources4
Helixi528 – 531Combined sources4
Helixi534 – 536Combined sources3
Beta strandi543 – 545Combined sources3
Beta strandi547 – 555Combined sources9
Helixi559 – 561Combined sources3
Beta strandi621 – 626Combined sources6
Turni627 – 630Combined sources4
Beta strandi631 – 633Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BB7X-ray2.40A/B/C/D401-641[»]
ProteinModelPortaliQ06488.
SMRiQ06488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 103Bromo 1PROSITE-ProRule annotationAdd BLAST69
Domaini295 – 365Bromo 2PROSITE-ProRule annotationAdd BLAST71
Domaini408 – 526BAHPROSITE-ProRule annotationAdd BLAST119

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi667 – 716Gln-richAdd BLAST50

Sequence similaritiesi

Belongs to the RSC1 family.Curated
Contains 1 BAH domain.PROSITE-ProRule annotation
Contains 2 bromo domains.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Repeat

Phylogenomic databases

GeneTreeiENSGT00390000003017.
HOGENOMiHOG000247903.
InParanoidiQ06488.
KOiK11756.
OMAiNRIRTWK.
OrthoDBiEOG092C1S4Z.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001025. BAH_dom.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
[Graphical view]
PfamiPF01426. BAH. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00439. BAH. 1 hit.
SM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS51038. BAH. 1 hit.
PS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMPDDNSNSS TQNSSALYKD LRKEYESLFT LKEDSGLEIS PIFNVLPPKK
60 70 80 90 100
DYPDYYAVIK NPVSFNTLKK RIPHYTDAQQ FMNDVVQIPW NAKTYNTRDS
110 120 130 140 150
GIYKYALVLE KYLKDTIYPN LKEKYPQLVY PDLGPLPDEP GYEEFQQKLR
160 170 180 190 200
EKAEEVARAN AARAESSSSM NSTEAARRLR KTRTSVKRES EPGTDTNNDE
210 220 230 240 250
DYEATDMDID NPKDADFPDL IRKPLININP YTRKPLRDNR STTPSHSGTP
260 270 280 290 300
QPLGPRHRQV SRTQVKRGRP PIIDLPYIQR MKNVMKVLKK EVLDSGIGLT
310 320 330 340 350
DLFERLPDRH RDANYYIMIA NPISLQDINK KVKTRRYKTF QEFQNDFNLM
360 370 380 390 400
LTNFRISHRG DPESIKISNI LEKTFTSLAR FELSKPDRSF IPEGELRYPL
410 420 430 440 450
DEVIVNNISY HVGDWALLRN QNDPQKPIVG QIFRLWKTPD GKQWLNACWY
460 470 480 490 500
YRPEQTVHRV DRLFYKNEVM KTGQYRDHLV SNLVGKCYVI HFTRYQRGNP
510 520 530 540 550
DMKLEGPLFV CEFRYNESDK IFNKIRTWKA CLPEEIRDLD EATIPVNGRK
560 570 580 590 600
FFKYPSPIRH LLPANATPHD RVPEPTMGSP DAPPLVGAVY MRPKMQRDDL
610 620 630 640 650
GEYATSDDCP RYIIRPNDSP EEGQVDIETG TITTNTPTAN ALPKTGYSSS
660 670 680 690 700
KLSSLRYNRS SMSLENQNAI GQQQIPLSRV GSPGAGGPLT VQGLKQHQLQ
710 720 730 740 750
RLQQQQHQYQ QQKRSQASRY NIPTIIDDLT SQASRGNLGN IMIDAASSFV
760 770 780 790 800
LPISITKNVD VLQRTDLHSQ TKRSGREEMF PWKKTKGEIL WFRGPSVIVN
810 820 830 840 850
ERIINSGDPH LSLPLNRWFT TNKKRKLEYE EVEETMEDVT GKDKDDDGLE
860 870 880
PDVENEKESL PGPFVLGLRP SAKFTAHRLS MLRPPSSSS
Length:889
Mass (Da):102,300
Last modified:November 1, 1996 - v1
Checksum:i7867866FD973F188
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19102 Genomic DNA. Translation: AAB67747.1.
BK006945 Genomic DNA. Translation: DAA09661.1.
PIRiS51465.
RefSeqiNP_013461.1. NM_001182246.1.

Genome annotation databases

EnsemblFungiiYLR357W; YLR357W; YLR357W.
GeneIDi851071.
KEGGisce:YLR357W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19102 Genomic DNA. Translation: AAB67747.1.
BK006945 Genomic DNA. Translation: DAA09661.1.
PIRiS51465.
RefSeqiNP_013461.1. NM_001182246.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BB7X-ray2.40A/B/C/D401-641[»]
ProteinModelPortaliQ06488.
SMRiQ06488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31619. 250 interactors.
DIPiDIP-863N.
IntActiQ06488. 53 interactors.
MINTiMINT-666605.

PTM databases

iPTMnetiQ06488.

Proteomic databases

MaxQBiQ06488.
PRIDEiQ06488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR357W; YLR357W; YLR357W.
GeneIDi851071.
KEGGisce:YLR357W.

Organism-specific databases

EuPathDBiFungiDB:YLR357W.
SGDiS000004349. RSC2.

Phylogenomic databases

GeneTreeiENSGT00390000003017.
HOGENOMiHOG000247903.
InParanoidiQ06488.
KOiK11756.
OMAiNRIRTWK.
OrthoDBiEOG092C1S4Z.

Enzyme and pathway databases

BioCyciYEAST:G3O-32429-MONOMER.

Miscellaneous databases

PROiQ06488.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001025. BAH_dom.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
[Graphical view]
PfamiPF01426. BAH. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00439. BAH. 1 hit.
SM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS51038. BAH. 1 hit.
PS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSC2_YEAST
AccessioniPrimary (citable) accession number: Q06488
Secondary accession number(s): D6VYZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2330 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.