Q06485 (ATG33_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Autophagy-related protein 33

Gene names

Name:	ATG33
Ordered Locus Names:	YLR356W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the selective degradation of mitochondria via autophagy during starvation and at post-log phase. Ref.7 Ref.8
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein Potential Ref.4.
Sequence similarities	Belongs to the ATG33 family.

Ontologies

Keywords
Biological process	Autophagy
Cellular component	Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrion degradation Inferred from mutant phenotype Ref.7. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay Ref.4Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	Autophagy-related protein 33		PRO_0000247213
Regions
	Transmembrane	10 – 30	21	Helical; Potential
	Transmembrane	52 – 72	21	Helical; Potential
	Transmembrane	78 – 98	21	Helical; Potential
	Transmembrane	172 – 192	21	Helical; Potential
Amino acid modifications
	Modified residue	127	1	Phosphoserine Ref.5 Ref.6
	Modified residue	129	1	Phosphoserine Ref.5 Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q06485 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4BA9F5CD485FB259	FASTA	197	20,400
	10 20 30 40 50 60 MSVCLAITKG IAVSSIGLYS GLLASASLIT STTPLEVLTG SLTPTLTTLK NAATALGAFA 70 80 90 100 110 120 STFFCVSFFG APPSLRHPYL LYGMLVAPLS SFVLGCASNY QSRKYSKVSK ESSLFPEDSK 130 140 150 160 170 180 LAASELSDSI IDLGEDNHAS ENTPRDGKPA ATTVSKPAEA LHTGPPIHTK NLIAATAIAI 190 VGFVQAVIGV YGEGQFI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-127 AND SER-129, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-127 AND SER-129, MASS SPECTROMETRY.
[7]	"A genomic screen for yeast mutants defective in selective mitochondria autophagy." Kanki T., Wang K., Baba M., Bartholomew C.R., Lynch-Day M.A., Du Z., Geng J., Mao K., Yang Z., Yen W.L., Klionsky D.J. Mol. Biol. Cell 20:4730-4738(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"A genomic screen for yeast mutants defective in mitophagy." Kanki T., Wang K., Klionsky D.J. Autophagy 6:278-280(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19102 Genomic DNA. Translation: AAB67748.1. AY558222 Genomic DNA. Translation: AAS56548.1. BK006945 Genomic DNA. Translation: DAA09660.1.
PIR	S51464.
RefSeq	NP_013460.1. NM_001182245.1.
3D structure databases
ProteinModelPortal	Q06485.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4664N.
IntAct	Q06485. 1 interaction.
MINT	MINT-481986.
STRING	4932.YLR356W.
Proteomic databases
PaxDb	Q06485.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR356W; YLR356W; YLR356W.
GeneID	851070.
KEGG	sce:YLR356W.
Organism-specific databases
CYGD	YLR356w.
SGD	S000004348. ATG33.
Phylogenomic databases
eggNOG	NOG68417.
GeneTree	ENSGT00530000067118.
OMA	NHASENT.
OrthoDB	EOG405W9X.
Gene expression databases
Genevestigator	Q06485.
GermOnline	YLR356W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	967714.

Entry information

Entry name

ATG33_YEAST

Accession

Primary (citable) accession number: Q06485
Secondary accession number(s): D6VYZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program