Reviewed,
UniProtKB/Swiss-Prot Q06481 (APLP2_HUMAN)
Last modified
December 15, 2009.
Version 109.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Amyloid-like protein 2 Alternative name(s): Amyloid protein homolog APPH CDEI box-binding protein Short name=CDEBP | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 763 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein. Ref.7 |
| Subunit structure | Interacts with CPEB1 By similarity. |
| Subcellular location | Cell membrane; Single-pass type I membrane protein Potential. Nucleus Potential. |
| Tissue specificity | In placenta, brain, heart, lung, liver, kidney and endothelial tissues. |
| Post-translational modification | The BPTI/Kunitz inhibitor domain is O-glycosylated. Ref.7 |
| Sequence similarities | Belongs to the APP family. Contains 1 BPTI/Kunitz inhibitor domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane Nucleus |
| Coding sequence diversity | Alternative splicing Polymorphism |
| Domain | Signal Transmembrane |
| Ligand | DNA-binding |
| Molecular function | Protease inhibitor Serine protease inhibitor |
| PTM | Disulfide bond Glycoprotein Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | G-protein coupled receptor protein signaling pathway Ref.2 Non-traceable author statement. Source: UniProtKB |
| Cellular component | integral to membrane Ref.1 Non-traceable author statement. Source: UniProtKB nucleus Ref.3Inferred from direct assay. Source: UniProtKB plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | DNA binding Ref.3 Non-traceable author statement. Source: UniProtKB identical protein bindingInferred from physical interaction. Source: IntAct serine-type endopeptidase inhibitor activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| itself | 2 | EBI-79306,EBI-79306 | ||
| APBB2 | Q92870 | 1 | EBI-79306,EBI-79277 | |
| APLP1 | P51693 | 1 | EBI-79306,EBI-74648 | |
| APP | P05067-4 | 1 | EBI-79306,EBI-302641 | |
| Slc5a7 | Q8BGY9 | 1 | EBI-79306,EBI-2010752 | From a different organism. |
| SPON1 | Q9HCB6 | 1 | EBI-79306,EBI-2431846 |
Alternative products
| This entry describes 5 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist. | ||||||
| Isoform 1 (identifier: Q06481-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q06481-2) The sequence of this isoform differs from the canonical sequence as follows: 308-363: Missing. | ||||||
| Isoform 3 (identifier: Q06481-3) The sequence of this isoform differs from the canonical sequence as follows: 613-624: Missing. | ||||||
| Isoform 4 (identifier: Q06481-4) The sequence of this isoform differs from the canonical sequence as follows: 308-363: Missing. 613-624: Missing. | ||||||
| Isoform 5 (identifier: Q06481-5) The sequence of this isoform differs from the canonical sequence as follows: 136-364: Missing. 613-624: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 29 | 29 | Potential | ||||||||
| Chain | 30 – 763 | 734 | Amyloid-like protein 2 | PRO_0000000207 | |||||||
Regions | |||||||||||
| Topological domain | 30 – 692 | 663 | Extracellular Potential | ||||||||
| Transmembrane | 693 – 716 | 24 | Potential | ||||||||
| Topological domain | 717 – 763 | 47 | Cytoplasmic Potential | ||||||||
| Domain | 306 – 364 | 59 | BPTI/Kunitz inhibitor | ||||||||
| Compositional bias | 215 – 280 | 66 | Asp/Glu-rich (highly acidic) | ||||||||
| Compositional bias | 215 – 231 | 17 | Poly-Glu | ||||||||
Sites | |||||||||||
| Site | 320 – 321 | 2 | Reactive bond By similarity | ||||||||
Amino acid modifications | |||||||||||
| Modified residue | 750 | 1 | Phosphotyrosine Ref.8 Ref.9 | ||||||||
| Modified residue | 755 | 1 | Phosphotyrosine Ref.8 | ||||||||
| Disulfide bond | 310 ↔ 360 | By similarity | |||||||||
| Disulfide bond | 319 ↔ 343 | By similarity | |||||||||
| Disulfide bond | 335 ↔ 356 | By similarity | |||||||||
Natural variations | |||||||||||
| Alternative sequence | 136 – 364 | 229 | Missing in isoform 5. | VSP_030921 | |||||||
| Alternative sequence | 308 – 363 | 56 | Missing in isoform 2 and isoform 4. | VSP_000018 | |||||||
| Alternative sequence | 613 – 624 | 12 | Missing in isoform 3, isoform 4 and isoform 5. | VSP_000019 | |||||||
| Natural variant | 632 | 1 | D → N: dbSNP rs3740881. | VAR_022039 | |||||||
Experimental info | |||||||||||
| Sequence conflict | 224 | 1 | E → D in AAD47291. Ref.5 | ||||||||
| Sequence conflict | 364 | 1 | I → V in AAA35601. Ref.4 | ||||||||
| Sequence conflict | 364 | 1 | I → V in AAA36130. Ref.4 | ||||||||
| Sequence conflict | 543 | 1 | S → I in AAA35526. Ref.1 | ||||||||
| Sequence conflict | 543 | 1 | S → T in AAH04371. Ref.6 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning of the cDNA for a human amyloid precursor protein homolog: evidence for a multigene family." Sprecher C.A., Grant F.J., Grimm G., O'Hara P.J., Norris F., Norris K., Foster D.C. Biochemistry 32:4481-4486(1993) [PubMed: 8485127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Placenta. |
| [2] | "Isolation and characterization of APLP2 encoding a homologue of the Alzheimer's associated amyloid beta protein precursor." Wasco W., Gurubhagavatula S., Paradis M., Romano D.M., Sisodia S.S., Hyman B.T., Neve R.L., Tanzi R.E. Nat. Genet. 5:95-99(1993) [PubMed: 8220435] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain. |
| [3] | "A human amyloid precursor-like protein is highly homologous to a mouse sequence-specific DNA-binding protein." von der Kammer H., Hanes J., Klaudiny J., Scheit K.H. DNA Cell Biol. 13:1137-1143(1994) [PubMed: 7702756] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Ovary. |
| [4] | "Cloning of a protein that binds to a recognition sequence in the APP promoter." Vostrov A.A., Quitschke W.W., Schwarzman A.L., Blangy A., Cuzin F., Wesley U.V., Hagag N.G., Goldgaber D. Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-763 (ISOFORM 2), NUCLEOTIDE SEQUENCE [MRNA] OF 229-763 (ISOFORM 3), NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4). Tissue: Brain. |
| [5] | "A new special gene in human testis (HSD-2)." Wang L., Miao S., Fan H., Wang H. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 5). Tissue: Lung and Pancreas. |
| [7] | "Expression, purification and characterization of a Kunitz-type protease inhibitor domain from human amyloid precursor protein homolog." Petersen L.C., Bjorn S.E., Norris F., Norris K., Sprecher C., Foster D.C. FEBS Lett. 338:53-57(1994) [PubMed: 8307156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 305-362 (ISOFORMS 1/3), FUNCTION, GLYCOSYLATION. |
| [8] | "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. Comb M.J.Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-750 AND TYR-755, MASS SPECTROMETRY. |
| [9] | "An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-750, MASS SPECTROMETRY. Tissue: Mammary epithelium. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| S60099 mRNA. Translation: AAC60589.1. L09209 mRNA. Translation: AAA35526.1. L27631 mRNA. Translation: AAC41701.1. Z22572 mRNA. Translation: CAA80295.1. L19597 mRNA. Translation: AAA35601.1. L23113 mRNA. Translation: AAA36032.1. L23114 mRNA. Translation: AAA36130.1. AF168956 mRNA. Translation: AAD47291.1. BC000373 mRNA. Translation: AAH00373.1. BC004371 mRNA. Translation: AAH04371.1. | |
| IPI | IPI00031030. IPI00220977. IPI00220978. IPI00334946. IPI00884988. |
| PIR | A49321. S41082. |
| RefSeq | NP_001135748.1. NP_001135749.1. NP_001135750.1. NP_001633.1. |
3D structure databases | |
| SMR | Q06481. Positions 47-139. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-31047N. |
| IntAct | Q06481. 8 interactions. |
| STRING | Q06481. |
Protein family/group databases | |
| MEROPS | I02.016. |
PTM databases | |
| PhosphoSite | Q06481. |
Proteomic databases | |
| PRIDE | Q06481. |
Genome annotation databases | |
| Ensembl | ENST00000263574; ENSP00000263574; ENSG00000084234; Homo sapiens. [Genome view] |
| GeneID | 334. |
| KEGG | hsa:334. |
| UCSC | uc001qfp.1. human. uc001qfq.1. human. uc001qfs.1. human. uc001qft.1. human. uc001qfv.1. human. |
Organism-specific databases | |
| CTD | 334. |
| GeneCards | GC11P129445. |
| HGNC | HGNC:598. APLP2. |
| MIM | 104776. gene. |
| PharmGKB | PA24885. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOVERGEN | Q06481. |
| InParanoid | Q06481. |
| OMA | LHTIRHY. |
| OrthoDB | EOG98PQ4R. |
Gene expression databases | |
| ArrayExpress | Q06481. |
| Bgee | Q06481. |
| CleanEx | HS_APLP2. HS_APPL2. |
| Genevestigator | Q06481. |
| GermOnline | ENSG00000084234. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR008155. Amyloid_glyco. IPR011178. Amyloid_glyco_Cu-bd. IPR008154. Amyloid_glyco_extra. IPR019744. Amyloid_glyco_extracell_CS. IPR015849. Amyloid_glyco_heparin-bd. IPR019745. Amyloid_glyco_intracell_CS. IPR019543. APP_amyloid. IPR002223. Prot_inh_Kunz-m. [Graphical view] |
| Gene3D | G3DSA:3.30.1490.140. Amyloid_glyco_Cu-bd. 1 hit. G3DSA:3.90.570.10. Amyloid_glyco_heparin-bd. 1 hit. G3DSA:4.10.410.10. Prot_inh_Kunz-m. 1 hit. |
| Pfam | PF02177. A4_EXTRA. 1 hit. PF10515. APP_amyloid. 1 hit. PF00014. Kunitz_BPTI. 1 hit. [Graphical view] |
| PRINTS | PR00203. AMYLOIDA4. PR00759. BASICPTASE. |
| SMART | SM00006. A4_EXTRA. 1 hit. SM00131. KU. 1 hit. [Graphical view] |
| PROSITE | PS00319. A4_EXTRA. 1 hit. PS00320. A4_INTRA. 1 hit. PS00280. BPTI_KUNITZ_1. 1 hit. PS50279. BPTI_KUNITZ_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 1383. |
| PMAP-CutDB | Q06481. |
| SOURCE | Search... |
Entry information
| Entry name | APLP2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q06481 Secondary accession number(s): Q13861 Q9BT36 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 11 Human chromosome 11: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


