Q06479 (YL352_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box protein YLR352W

Gene names

Ordered Locus Names:

YLR352W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SKP1 and CDC53. Component of the probable SCF(YBR352W) complex containing CDC53, SKP1, RBX1 and YBR352W. Ref.3
Miscellaneous	Present with 125 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	SCF ubiquitin ligase complex Inferred from direct assay Ref.3. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKP1	P52286	4	EBI-35627,EBI-4090

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	F-box protein YLR352W		PRO_0000268189
Regions
	Domain	220 – 266	47	F-box

Sequences

	Sequence		Length	Mass (Da)
	Q06479 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A44090288065A7BD	FASTA	807	93,123
	10 20 30 40 50 60 MPDLKSKTLS SQSLGAAIPP EIVYQILTYQ FRDLLRNDHP GTAEKFNENL TTFVKSNLTV 70 80 90 100 110 120 NKTFSHICQV LIYRYCNLTT AKRFHGLLQT LKGNRCLCNK IEVADFQELT SIGLGRSSEM 130 140 150 160 170 180 NKMIKNLTNE TLLEFLMLTK ANLREFLACE NIQDDLDDNI IKYILSPGKV LSVVDFCGCS 190 200 210 220 230 240 GTTFTESFIK ALDKYYPNKS IDQYRLEPIQ QNYQITCLGL NDCIDLPSHV LWKILKMLPE 250 260 270 280 290 300 LQKLDLSHTS IDDSTLYHGI PHWKNLTHLS LATCLQVTPR AVLEFFSHHP TITDPDNTST 310 320 330 340 350 360 LEWLNVSVIA HSSSWNEVHT MFLLKKLCQH GHNKTLQYLN IGGLPLHVAP SFGEDPISES 370 380 390 400 410 420 TYYYQCRDSL QFIKWNFPKL KSLSIKGNSI PISTLVEFLT PIDQDHPNCA QKLKFLNISG 430 440 450 460 470 480 NSYVNKWTIQ DSLLYTCSPS LVALEVSFDS WQQIEKLNDR HEIIAYRYKN PNSVIKDIST 490 500 510 520 530 540 AEQVKWKCYI DSSYGRRYWL YKTDPFLNRD DLDSKSNLTR YDFEGHKIIE IINQPDFLKF 550 560 570 580 590 600 AQSKIMLGCG LVPQSGIRRK LCYRDLKPPV SQFLNRKGAI SLGDTPLPII TPTLPRGGWR 610 620 630 640 650 660 IIHNEDDNNS HVEDSQNHVN AIPRRNSLLS RPTLRSNNGS SSANPFAINV SPASQIRDGL 670 680 690 700 710 720 YWDRSVHDLR ELSLQEQRIQ ELADEQQELR TIANFEETDD EYLHDPDLQR RRSQLHLFES 730 740 750 760 770 780 SRSRSGNKTR PSLTGEHSSS ASFLSFSHFN HLHKRKNYYF THPDEFVYDP KDPLTTQRYR 790 800 LHFEIVNEYQ VFGCIERGMY RYYSLKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly." Seol J.H., Shevchenko A., Shevchenko A., Deshaies R.J. Nat. Cell Biol. 3:384-391(2001) [PubMed: 11283612] [Abstract] Cited for: INTERACTION WITH CDC53 AND SKP1, IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN F-BOX.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19102 Genomic DNA. Translation: AAB67750.1. BK006945 Genomic DNA. Translation: DAA09656.1.
PIR	S51460.
RefSeq	NP_013456.1. NM_001182241.1.
3D structure databases
ProteinModelPortal	Q06479.
SMR	Q06479. Positions 213-271, 370-447.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1631N.
IntAct	Q06479. 4 interactions.
MINT	MINT-400372.
STRING	Q06479.
Proteomic databases
PeptideAtlas	Q06479.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR352W; YLR352W; YLR352W.
GeneID	851066.
KEGG	sce:YLR352W.
NMPDR	fig|4932.3.peg.4478.
Organism-specific databases
CYGD	YLR352w.
SGD	S000004344. YLR352W.
Phylogenomic databases
eggNOG	fuNOG08212.
GeneTree	EFGT00050000006558.
HOGENOM	HBG202551.
OMA	ERGMYRY.
OrthoDB	EOG4VX5DJ.
Gene expression databases
ArrayExpress	Q06479.
Genevestigator	Q06479.
GermOnline	YLR352W. Saccharomyces cerevisiae.
Family and domain databases
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	967702.

Entry information

Entry name

YL352_YEAST

Accession

Primary (citable) accession number: Q06479
Secondary accession number(s): D6VYZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program