Reviewed, UniProtKB/Swiss-Prot Q06479 (YL352_YEAST)

Names and origin

Protein names

Recommended name:
    F-box protein YLR352W

Gene names

Ordered Locus Names:

YLR352W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SKP1 and CDC53. Component of the probable SCF(YBR352W) complex containing CDC53, SKP1, RBX1 and YBR352W. Ref.2
Miscellaneous	Present with 125 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Contains 1 F-box domain. Contains 3 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Leucine-rich repeat Repeat
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKP1	P52286	1	EBI-35627,EBI-4090

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	F-box protein YLR352W		PRO_0000268189
Regions
	Repeat	1 – 25	25	LRR 1
	Domain	220 – 266	47	F-box
	Repeat	377 – 400	24	LRR 2
	Repeat	666 – 691	26	LRR 3

Sequences

	Sequence		Length	Mass (Da)
	Q06479-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A44090288065A7BD	FASTA	807	93,123
	10 20 30 40 50 60 MPDLKSKTLS SQSLGAAIPP EIVYQILTYQ FRDLLRNDHP GTAEKFNENL TTFVKSNLTV 70 80 90 100 110 120 NKTFSHICQV LIYRYCNLTT AKRFHGLLQT LKGNRCLCNK IEVADFQELT SIGLGRSSEM 130 140 150 160 170 180 NKMIKNLTNE TLLEFLMLTK ANLREFLACE NIQDDLDDNI IKYILSPGKV LSVVDFCGCS 190 200 210 220 230 240 GTTFTESFIK ALDKYYPNKS IDQYRLEPIQ QNYQITCLGL NDCIDLPSHV LWKILKMLPE 250 260 270 280 290 300 LQKLDLSHTS IDDSTLYHGI PHWKNLTHLS LATCLQVTPR AVLEFFSHHP TITDPDNTST 310 320 330 340 350 360 LEWLNVSVIA HSSSWNEVHT MFLLKKLCQH GHNKTLQYLN IGGLPLHVAP SFGEDPISES 370 380 390 400 410 420 TYYYQCRDSL QFIKWNFPKL KSLSIKGNSI PISTLVEFLT PIDQDHPNCA QKLKFLNISG 430 440 450 460 470 480 NSYVNKWTIQ DSLLYTCSPS LVALEVSFDS WQQIEKLNDR HEIIAYRYKN PNSVIKDIST 490 500 510 520 530 540 AEQVKWKCYI DSSYGRRYWL YKTDPFLNRD DLDSKSNLTR YDFEGHKIIE IINQPDFLKF 550 560 570 580 590 600 AQSKIMLGCG LVPQSGIRRK LCYRDLKPPV SQFLNRKGAI SLGDTPLPII TPTLPRGGWR 610 620 630 640 650 660 IIHNEDDNNS HVEDSQNHVN AIPRRNSLLS RPTLRSNNGS SSANPFAINV SPASQIRDGL 670 680 690 700 710 720 YWDRSVHDLR ELSLQEQRIQ ELADEQQELR TIANFEETDD EYLHDPDLQR RRSQLHLFES 730 740 750 760 770 780 SRSRSGNKTR PSLTGEHSSS ASFLSFSHFN HLHKRKNYYF THPDEFVYDP KDPLTTQRYR 790 800 LHFEIVNEYQ VFGCIERGMY RYYSLKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly." Seol J.H., Shevchenko A., Shevchenko A., Deshaies R.J. Nat. Cell Biol. 3:384-391(2001) [PubMed: 11283612] [Abstract] Cited for: INTERACTION WITH CDC53 AND SKP1, IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN F-BOX.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U19102 Genomic DNA. Translation: AAB67750.1.
PIR	S51460.
RefSeq	NP_013456.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1631N.
IntAct	Q06479. 5 interactions.
Proteomic databases
PeptideAtlas	Q06479.
PRIDE	Q06479.
Genome annotation databases
Ensembl	YLR352W. Saccharomyces cerevisiae. [Contig view]
GeneID	851066.
GenomeReviews	Gene locus YLR352W in contig Y13138_GR.
KEGG	sce:YLR352W.
NMPDR	fig|4932.3.peg.4478.
Organism-specific databases
CYGD	YLR352w.
SGD	S000004344. YLR352W.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q06479.
OMA	Q06479. ERGMYRY.
Gene expression databases
ArrayExpress	Q06479.
GermOnline	YLR352W. Saccharomyces cerevisiae.
Family and domain databases
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967702.

Entry information

Entry name

YL352_YEAST

Accession

Primary (citable) accession number: Q06479

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)