Reviewed, UniProtKB/Swiss-Prot Q06478 (PA11_DOLMA)

Names and origin

Protein names	Recommended name:     Phospholipase A1 1     EC=3.1.1.32     EC=3.1.1.4 Alternative name(s):     Allergen Dol m I     Allergen=Dol m 1.01
Organism	Dolichovespula maculata (Bald-faced hornet) (Vespula maculata)
Taxonomic identifier	7441 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Vespinae › Dolichovespula

Protein attributes

Sequence length	317 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities.
Catalytic activity	Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate. Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	Contains six disulfide bonds By similarity.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phospholipase A1 activity Inferred from electronic annotation. Source: EC phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 17	›17
	Chain	18 – 317	300	Phospholipase A1 1		PRO_0000017812
Sites
	Active site	154	1	Nucleophile By similarity
	Active site	182	1	Charge relay system By similarity
	Active site	246	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	25	1	N-linked (GlcNAc...)
	Glycosylation	229	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	271	1	N → S
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q06478-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 0B2135FD453512D9	FASTA	317	35,708
	10 20 30 40 50 60 RLIMFVGDPS SSNELDRFSV CPFSNDTVKM IFLTRENRKH DFYTLDTMNR HNEFKKSIIK 70 80 90 100 110 120 RPVVFITHGF TSSATEKNFV AMSEALMHTG DFLIIMVDWR MAACTDEYPG LKYMFYKAAV 130 140 150 160 170 180 GNTRLVGNFI AMIAKKLVEQ YKVPMTNIRL VGHSLGAHIS GFAGKRVQEL KLGKFSEIIG 190 200 210 220 230 240 LDPAGPSFKK NDCSERICET DAHYVQILHT SSNLGTERTL GTVDFYINNG SNQPGCRYII 250 260 270 280 290 300 GETCSHTRAV KYFTECIRRE CCLIGVPQSK NPQPVSKCTR NECVCVGLNA KKYPKRGSFY 310 VPVEAEAPYC NNNGKII

References

[1]	"Sequence similarity of a hornet (D. maculata) venom allergen phospholipase A1 with mammalian lipases." Soldatova L., Kochoumian L., King T.P. FEBS Lett. 320:145-149(1993) [PubMed: 8458431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Venom and Venom gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X66869 mRNA. Translation: CAA47341.1.
PIR	S32406.
3D structure databases
HSSP	HSSP built from PDB template 1BU8 based on UniProtKB P54318.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.32. 299019. 3.1.1.4. 299019.
Family and domain databases
InterPro	IPR002334. Dol/Ves_allerg. IPR000734. Lipase. IPR013818. Lipase_N. [Graphical view]
PANTHER	PTHR11610. Lipase. 1 hit.
Pfam	PF00151. Lipase. 1 hit. [Graphical view]
PRINTS	PR00825. DOLALLERGEN.
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA11_DOLMA

Accession

Primary (citable) accession number: Q06478

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)