Q06474 (COX2_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase subunit 2
EC=1.9.3.1

Alternative name(s):
Cytochrome aa3 subunit 2
Cytochrome c oxidase polypeptide II
Oxidase aa(3) subunit 2

Gene names

Name:	ctaC
Synonyms:	coxB
Ordered Locus Names:	slr1136

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B).
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Cofactor	Copper A.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cytochrome c oxidase subunit 2 family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	copper ion binding Inferred from electronic annotation. Source: InterPro cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 332	312	Cytochrome c oxidase subunit 2		PRO_0000006065
Regions
	Transmembrane	48 – 68	21	Helical; Potential
	Transmembrane	87 – 107	21	Helical; Potential
Sites
	Metal binding	214	1	Copper A Probable
	Metal binding	249	1	Copper A Probable
	Metal binding	253	1	Copper A Probable
	Metal binding	257	1	Copper A Probable
Experimental info
	Sequence conflict	240 – 244	5	STPGQ → RHPWA Ref.1
	Sequence conflict	240 – 244	5	STPGQ → RHPWA Ref.2
	Sequence conflict	320 – 332	13	QLKDQ…VGDLL → N Ref.1
	Sequence conflict	320 – 332	13	QLKDQ…VGDLL → N Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q06474 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 314F15C8F8D4AD26	FASTA	332	35,917
	10 20 30 40 50 60 MKIPGSVITL LIGVVITVVS LWYGQNHGLM PVAASADAEK VDGIFNYMMT IATGLFLLVE 70 80 90 100 110 120 GVLVYCLIRF RRRKDDQTDG PPIEGNVPLE ILWTAIPTVI VFTLAVYSFE VYNNLGGLDP 130 140 150 160 170 180 TISRDNAGQQ MAHNHMGHMG SMGNMVAMAG DGDVALGIGL DSEEQGVNPL MVDVKGIQYA 190 200 210 220 230 240 WIFTYPETGI ISGELHAPID RPVQLNMEAG DVIHAFWIPQ LRLKQDVIPG RGSTLVFNAS 250 260 270 280 290 300 TPGQYPVICA ELCGAYHGGM KSVFYAHTPE EYDDWVAANA PAPTESMAMT LPKATTAMTP 310 320 330 NEYLAPYAKE MGVQTEALAQ LKDQTSPVGD LL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of the ctaC (coxB) gene as part of an operon encoding subunits I, II, and III of the cytochrome c oxidase (cytochrome aa3) in the cyanobacterium Synechocystis PCC 6803." Alge D., Peschek G.A. Biochem. Biophys. Res. Commun. 191:9-17(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of a cta/CDE operon-like genomic region encoding subunits I-III of the cytochrome c oxidase of the cyanobacterium Synechocystis PCC 6803." Alge D., Peschek G.A. Biochem. Mol. Biol. Int. 29:511-525(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53746 Genomic DNA. Translation: CAA37776.1. BA000022 Genomic DNA. Translation: BAA17288.1.
PIR	S77441.
RefSeq	NP_440608.1. NC_000911.1. YP_005650666.1. NC_017277.1. YP_007450491.1. NC_020286.1.
3D structure databases
ProteinModelPortal	Q06474.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q06474. 2 interactions.
STRING	1148.slr1136.
Proteomic databases
PaxDb	Q06474.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17288; BAA17288; BAA17288.
GeneID	12256358. 14616141. 953911.
KEGG	syn:slr1136. syy:SYNGTS_0713.
PATRIC	23838410. VBISynSp132158_0772.
Phylogenomic databases
eggNOG	COG1622.
HOGENOM	HOG000232614.
KO	K02275.
OMA	GQPTILN.
ProtClustDB	CLSK892771.
Family and domain databases
Gene3D	1.10.287.90. 1 hit. 2.60.40.420. 1 hit.
InterPro	IPR001505. Copper_CuA. IPR008972. Cupredoxin. IPR002429. Cyt_c_oxidase_su2_C. IPR011759. Cyt_c_oxidase_su2_TM_dom. [Graphical view]
Pfam	PF00116. COX2. 1 hit. PF02790. COX2_TM. 1 hit. [Graphical view]
SUPFAM	SSF49503. Cupredoxin. 1 hit. SSF81464. Cyt_c_oxidase_II-like_TM. 1 hit.
PROSITE	PS00078. COX2. 1 hit. PS50857. COX2_CUA. 1 hit. PS50999. COX2_TM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COX2_SYNY3

Accession

Primary (citable) accession number: Q06474
Secondary accession number(s): P77963

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program