Reviewed, UniProtKB/Swiss-Prot Q06458 (NIRB_KLEOX)

Names and origin

Protein names

Recommended name:
    Nitrite reductase [NAD(P)H] large subunit
    EC=1.7.1.4

Gene names

Name:

nasB

Organism

Klebsiella oxytoca

Taxonomic identifier

571 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	957 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H.
Cofactor	Binds 1 siroheme per subunit. Binds 1 4Fe-4S cluster per subunit By similarity. FAD.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation).
Subunit structure	Homodimer which associates with nirD By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	4Fe-4S FAD Flavoprotein Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: InterPro 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro nitrite reductase [NAD(P)H] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 957	957	Nitrite reductase [NAD(P)H] large subunit		PRO_0000199963
Regions
	Nucleotide binding	44 – 79	36	FAD Potential
	Nucleotide binding	193 – 225	33	NAD or NADP Potential
Sites
	Metal binding	639	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	645	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	679	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	683	1	Iron (siroheme axial ligand) By similarity
	Metal binding	683	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06458-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: A136CC7FA28C6631	FASTA	957	104,227
	10 20 30 40 50 60 MTKPVLVLVG HGMVGHHFLE QCVSRDLHQQ YRIVVFCEER YAAYDRVHLT EYFAGRSAES 70 80 90 100 110 120 LSLVEGDFFT QHGIELRLSE SVASIDREAR VVRDAFGHET HWDKLVLATG SYPFVPPVPG 130 140 150 160 170 180 HNLEGCFVYR TLDDLDQIAA RAATARRGVV IGGGLLGLEA ANALKQLGLE THVVEFAPNL 190 200 210 220 230 240 MAVQLDNGGA AMLREKISEL GVGVHTSKAT TEIVRNEQGL QLNFRDGSSL ATDMLVFSAG 250 260 270 280 290 300 IRPQDALARS GGLSVGERGG ICIDNQCRTS DPDVLAIGEC ALWENKIYGL VAPGYQMAAR 310 320 330 340 350 360 RAATLAGEAG SFSGADMSTK LKLLGVDVAS FGDAQGRTPG CQSYQWTHGP QQVYKKIVVS 370 380 390 400 410 420 ADGKNLLGGV LVGDAGDYAT LLQMMLNGMA LPKHPESLIL PALEGSRPKA LGVAALPDGA 430 440 450 460 470 480 QICSCHNVSK GDICQAVSGG AGDMAAIKSR TKAATGCGGC SALVKQVMEY QLAEQGVEVK 490 500 510 520 530 540 KDICEHFPWS RQEIYHLVRV NHIRTFEQLI ARYGQGHGCE VCKPLVASVL ASCWNEYLLK 550 560 570 580 590 600 PAHLPLQDTN DRYFANIQKD GTYSVVPRMA AGEVTPDGLI AIGQIAKRYQ LYSKVTGGQR 610 620 630 640 650 660 IDLFGARLEQ LPAIWRELAE AGFETGHAYG KSLRTVKSCV GSTWCRYGVQ DSTGLAVTLE 670 680 690 700 710 720 HRYKGLRAPH KIKMAVSGCT RECAEAQGKD IGVIATEKGW NLYVCGNGGM KPRHADLFAS 730 740 750 760 770 780 DLDEATLIRS IDRLLMFYIR TADRLQRTST WMDNLEGGVD YLREMILEDS LGIGEELEQE 790 800 810 820 830 840 MARVVESYQC EWQTTLNDPQ RLALFRSYVN SDEPDETVQR QTLRGQPQLA PFAAQGEPAL 850 860 870 880 890 900 PSRPWQAICD LDAIPQQAGI GARLGERQIA LFRFGDQVYA LDNLEPGSEA NVLSRGLLGD 910 920 930 940 950 AGGEPIVISP LYKQRIRLRD GRQCDGGEQA VRAWPVKVEN GKVWVGNQQL LARAEAS

References

[1]	"Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al." Lin J.T., Goldman B.S., Stewart V. J. Bacteriol. 175:2370-2378(1993) [PubMed: 8468296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M5a1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L06800 Genomic DNA. Translation: AAA25099.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.1.4. 81705.
Family and domain databases
InterPro	IPR007419. BFD_Fer2_bd. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR012744. Nitri_red_NirB. IPR012748. Nitri_red_NirD. IPR017881. Nitrite_Rdtase_lsu_NirD_bac. IPR001327. Pyr_OxRdtase_NAD_bd. IPR017941. Rieske_2Fe-2S. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
Pfam	PF04324. Fer2_BFD. 1 hit. PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 1 hit. PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00397. SIROHAEM.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02378. nirD_assim_sml. 1 hit. TIGR02374. nitri_red_nirB. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. PS51300. NIRD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIRB_KLEOX

Accession

Primary (citable) accession number: Q06458

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)