Q06457 (NASA_KLEOX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrate reductase
EC=1.7.99.4

Gene names

Name:

nasA

Organism

Klebsiella oxytoca

Taxonomic identifier

571 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	866 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster Potential. Molybdenum (molybdopterin).
Pathway	Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 1/4.
Induction	By nitrate or nitrite during nitrogen-limited growth.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	denitrification pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 866	866	Nitrate reductase		PRO_0000063235
Sites
	Metal binding	8	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	11	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	15	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	43	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06457 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: 2B6B464578784DD5	FASTA	866	93,897
	10 20 30 40 50 60 MTETRTTCPY CGVGCGVIAS RAPHGQVSVR GDEQHPANFG RLCVKGAALG ETVGLEGRML 70 80 90 100 110 120 FPEVDGERAT WPQALAAAGS RLREIIDRHG PQAVAFYASG QLLTEDYYAA NKLMKGFIGA 130 140 150 160 170 180 ANIDTNSRLC MSSAVTGYKR ALGADVVPCS YEDVENSDLV VLVGSNAAWA HPVLYQRLAQ 190 200 210 220 230 240 AKRDNPQMRV VVIDPRRTAT CDIADRHLAL APGSDGGLFV GLLNAIAASG AISDDFNDAQ 250 260 270 280 290 300 RALTIAQDWD LDKVAQFCGL PRQQIADFYR EFIAAPRAIT LYTMGINQSA SGSDKCNAII 310 320 330 340 350 360 NVHLACGKYG RPGCGPFSLT GQPNAMGGRE VGGLATMLAA HMNFEPDDLR RLARFWGSER 370 380 390 400 410 420 LAQTPGLTGV ELFAAIGRGE VKAVWIMGTN PVVSLPDSHA VSEALARCPL VIISDVVADT 430 440 450 460 470 480 DTGRFAHIRF PALAWGEKSG TVTNSERRIS RQRAFMPPPG EARADWWIVA RVAEALGFGS 490 500 510 520 530 540 AFAWQHPHEV FSEHAALSGY ENDGQRAFDI GGLADLSREA WDALEPVRWP VSRSEAAWSV 550 560 570 580 590 600 HKGWHRDGKL RMVPVAPQPT RATTDAFYPL ILNSGRIRDQ WHTMTRTGAV PRLMQHINEP 610 620 630 640 650 660 VVEVAPADAQ RYHLLEGELA RVRSPKGVMV AKVTIGDGQR PGSLFVPMHW NNQFARQGRV 670 680 690 700 710 720 NNLLAAVTDP HSGQPESKQT AVAIATWLPA WKGELFSRQP VPLPASLHWR RRAAQGIIHL 730 740 750 760 770 780 SLAGDTRSRD WLVEWCQRQG WQMQVAEGGK VWNLLAWRAG ELMLGWWSDA SEPAIDADWI 790 800 810 820 830 840 HAAFRVPPQN AARRHALLSG RKGGVEMPRG RIICSCFSVG ERAIGEAIAG GCRTPGALGG 850 860 KLKCGTNCGS CIPELKALLA AKLAQA

References

[1]	"Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al." Lin J.T., Goldman B.S., Stewart V. J. Bacteriol. 175:2370-2378(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M5a1.
[2]	Stewart V. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L06800 Genomic DNA. Translation: AAA25100.2.
3D structure databases
ProteinModelPortal	Q06457.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13446.
UniPathway	UPA00652; UER00706.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR007419. BFD-like_2Fe2S-bd_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. [Graphical view]
Pfam	PF04324. Fer2_BFD. 1 hit. PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NASA_KLEOX

Accession

Primary (citable) accession number: Q06457

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	August 14, 2001
Last modified:	May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program