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Q06457

- NASA_KLEOX

UniProt

Q06457 - NASA_KLEOX

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Protein
Nitrate reductase
Gene
nasA
Organism
Klebsiella oxytoca
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Binds 1 4Fe-4S cluster Reviewed prediction.
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Iron-sulfur (4Fe-4S) By similarity
Metal bindingi11 – 111Iron-sulfur (4Fe-4S) By similarity
Metal bindingi15 – 151Iron-sulfur (4Fe-4S) By similarity
Metal bindingi43 – 431Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. molybdenum ion binding Source: InterPro
  3. nitrate reductase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. denitrification pathway Source: UniProtKB-UniPathway
  2. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13446.
RETL1328306-WGS:GSTH-1824-MONOMER.
UniPathwayiUPA00652; UER00706.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:nasA
OrganismiKlebsiella oxytoca
Taxonomic identifieri571 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 866866Nitrate reductase
PRO_0000063235Add
BLAST

Expressioni

Inductioni

By nitrate or nitrite during nitrogen-limited growth.

Structurei

3D structure databases

ProteinModelPortaliQ06457.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 57574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR007419. BFD-like_2Fe2S-bd_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view]
PfamiPF04324. Fer2_BFD. 1 hit.
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06457-1 [UniParc]FASTAAdd to Basket

« Hide

MTETRTTCPY CGVGCGVIAS RAPHGQVSVR GDEQHPANFG RLCVKGAALG    50
ETVGLEGRML FPEVDGERAT WPQALAAAGS RLREIIDRHG PQAVAFYASG 100
QLLTEDYYAA NKLMKGFIGA ANIDTNSRLC MSSAVTGYKR ALGADVVPCS 150
YEDVENSDLV VLVGSNAAWA HPVLYQRLAQ AKRDNPQMRV VVIDPRRTAT 200
CDIADRHLAL APGSDGGLFV GLLNAIAASG AISDDFNDAQ RALTIAQDWD 250
LDKVAQFCGL PRQQIADFYR EFIAAPRAIT LYTMGINQSA SGSDKCNAII 300
NVHLACGKYG RPGCGPFSLT GQPNAMGGRE VGGLATMLAA HMNFEPDDLR 350
RLARFWGSER LAQTPGLTGV ELFAAIGRGE VKAVWIMGTN PVVSLPDSHA 400
VSEALARCPL VIISDVVADT DTGRFAHIRF PALAWGEKSG TVTNSERRIS 450
RQRAFMPPPG EARADWWIVA RVAEALGFGS AFAWQHPHEV FSEHAALSGY 500
ENDGQRAFDI GGLADLSREA WDALEPVRWP VSRSEAAWSV HKGWHRDGKL 550
RMVPVAPQPT RATTDAFYPL ILNSGRIRDQ WHTMTRTGAV PRLMQHINEP 600
VVEVAPADAQ RYHLLEGELA RVRSPKGVMV AKVTIGDGQR PGSLFVPMHW 650
NNQFARQGRV NNLLAAVTDP HSGQPESKQT AVAIATWLPA WKGELFSRQP 700
VPLPASLHWR RRAAQGIIHL SLAGDTRSRD WLVEWCQRQG WQMQVAEGGK 750
VWNLLAWRAG ELMLGWWSDA SEPAIDADWI HAAFRVPPQN AARRHALLSG 800
RKGGVEMPRG RIICSCFSVG ERAIGEAIAG GCRTPGALGG KLKCGTNCGS 850
CIPELKALLA AKLAQA 866
Length:866
Mass (Da):93,897
Last modified:August 14, 2001 - v2
Checksum:i2B6B464578784DD5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06800 Genomic DNA. Translation: AAA25100.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06800 Genomic DNA. Translation: AAA25100.2 .

3D structure databases

ProteinModelPortali Q06457.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00652 ; UER00706 .
BioCyci MetaCyc:MONOMER-13446.
RETL1328306-WGS:GSTH-1824-MONOMER.

Family and domain databases

InterProi IPR009010. Asp_de-COase-like_dom.
IPR007419. BFD-like_2Fe2S-bd_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view ]
Pfami PF04324. Fer2_BFD. 1 hit.
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al."
    Lin J.T., Goldman B.S., Stewart V.
    J. Bacteriol. 175:2370-2378(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: M5a1.
  2. Stewart V.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiNASA_KLEOX
AccessioniPrimary (citable) accession number: Q06457
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: August 14, 2001
Last modified: July 9, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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