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Q06457

- NASA_KLEOX

UniProt

Q06457 - NASA_KLEOX

Protein

Nitrate reductase

Gene

nasA

Organism
Klebsiella oxytoca
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (14 Aug 2001)
      Previous versions | rss
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    Functioni

    Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.

    Cofactori

    Binds 1 4Fe-4S cluster.Curated
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi8 – 81Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi11 – 111Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi15 – 151Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi43 – 431Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. molybdenum ion binding Source: InterPro
    3. nitrate reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. denitrification pathway Source: UniProtKB-UniPathway
    2. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Nitrate assimilation

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13446.
    RETL1328306-WGS:GSTH-1824-MONOMER.
    UniPathwayiUPA00652; UER00706.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrate reductase (EC:1.7.99.4)
    Gene namesi
    Name:nasA
    OrganismiKlebsiella oxytoca
    Taxonomic identifieri571 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 866866Nitrate reductasePRO_0000063235Add
    BLAST

    Expressioni

    Inductioni

    By nitrate or nitrite during nitrogen-limited growth.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06457.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 57574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR007419. BFD-like_2Fe2S-bd_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    [Graphical view]
    PfamiPF04324. Fer2_BFD. 1 hit.
    PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q06457-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTETRTTCPY CGVGCGVIAS RAPHGQVSVR GDEQHPANFG RLCVKGAALG    50
    ETVGLEGRML FPEVDGERAT WPQALAAAGS RLREIIDRHG PQAVAFYASG 100
    QLLTEDYYAA NKLMKGFIGA ANIDTNSRLC MSSAVTGYKR ALGADVVPCS 150
    YEDVENSDLV VLVGSNAAWA HPVLYQRLAQ AKRDNPQMRV VVIDPRRTAT 200
    CDIADRHLAL APGSDGGLFV GLLNAIAASG AISDDFNDAQ RALTIAQDWD 250
    LDKVAQFCGL PRQQIADFYR EFIAAPRAIT LYTMGINQSA SGSDKCNAII 300
    NVHLACGKYG RPGCGPFSLT GQPNAMGGRE VGGLATMLAA HMNFEPDDLR 350
    RLARFWGSER LAQTPGLTGV ELFAAIGRGE VKAVWIMGTN PVVSLPDSHA 400
    VSEALARCPL VIISDVVADT DTGRFAHIRF PALAWGEKSG TVTNSERRIS 450
    RQRAFMPPPG EARADWWIVA RVAEALGFGS AFAWQHPHEV FSEHAALSGY 500
    ENDGQRAFDI GGLADLSREA WDALEPVRWP VSRSEAAWSV HKGWHRDGKL 550
    RMVPVAPQPT RATTDAFYPL ILNSGRIRDQ WHTMTRTGAV PRLMQHINEP 600
    VVEVAPADAQ RYHLLEGELA RVRSPKGVMV AKVTIGDGQR PGSLFVPMHW 650
    NNQFARQGRV NNLLAAVTDP HSGQPESKQT AVAIATWLPA WKGELFSRQP 700
    VPLPASLHWR RRAAQGIIHL SLAGDTRSRD WLVEWCQRQG WQMQVAEGGK 750
    VWNLLAWRAG ELMLGWWSDA SEPAIDADWI HAAFRVPPQN AARRHALLSG 800
    RKGGVEMPRG RIICSCFSVG ERAIGEAIAG GCRTPGALGG KLKCGTNCGS 850
    CIPELKALLA AKLAQA 866
    Length:866
    Mass (Da):93,897
    Last modified:August 14, 2001 - v2
    Checksum:i2B6B464578784DD5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06800 Genomic DNA. Translation: AAA25100.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06800 Genomic DNA. Translation: AAA25100.2 .

    3D structure databases

    ProteinModelPortali Q06457.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00652 ; UER00706 .
    BioCyci MetaCyc:MONOMER-13446.
    RETL1328306-WGS:GSTH-1824-MONOMER.

    Family and domain databases

    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR007419. BFD-like_2Fe2S-bd_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    [Graphical view ]
    Pfami PF04324. Fer2_BFD. 1 hit.
    PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al."
      Lin J.T., Goldman B.S., Stewart V.
      J. Bacteriol. 175:2370-2378(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: M5a1.
    2. Stewart V.
      Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.

    Entry informationi

    Entry nameiNASA_KLEOX
    AccessioniPrimary (citable) accession number: Q06457
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3