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Protein

Polyamine transporter 3

Gene

TPO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermine, but not spermidine.2 Publications

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • spermine transmembrane transporter activity Source: SGD

GO - Biological processi

  • spermine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34287-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.66. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine transporter 3
Gene namesi
Name:TPO3
Ordered Locus Names:YPR156C
ORF Names:P9584.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR156C.
SGDiS000006360. TPO3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 182182CytoplasmicSequence analysisAdd
BLAST
Transmembranei183 – 20321HelicalSequence analysisAdd
BLAST
Topological domaini204 – 21512ExtracellularSequence analysisAdd
BLAST
Transmembranei216 – 23621HelicalSequence analysisAdd
BLAST
Topological domaini237 – 2459CytoplasmicSequence analysis
Transmembranei246 – 26621HelicalSequence analysisAdd
BLAST
Topological domaini267 – 2759ExtracellularSequence analysis
Transmembranei276 – 29621HelicalSequence analysisAdd
BLAST
Topological domaini297 – 3059CytoplasmicSequence analysis
Transmembranei306 – 32621HelicalSequence analysisAdd
BLAST
Topological domaini327 – 3359ExtracellularSequence analysis
Transmembranei336 – 35621HelicalSequence analysisAdd
BLAST
Topological domaini357 – 41660CytoplasmicSequence analysisAdd
BLAST
Transmembranei417 – 43721HelicalSequence analysisAdd
BLAST
Topological domaini438 – 4469ExtracellularSequence analysis
Transmembranei447 – 46721HelicalSequence analysisAdd
BLAST
Topological domaini468 – 48720CytoplasmicSequence analysisAdd
BLAST
Transmembranei488 – 50821HelicalSequence analysisAdd
BLAST
Topological domaini509 – 5124ExtracellularSequence analysis
Transmembranei513 – 53321HelicalSequence analysisAdd
BLAST
Topological domaini534 – 55017CytoplasmicSequence analysisAdd
BLAST
Transmembranei551 – 57121HelicalSequence analysisAdd
BLAST
Topological domaini572 – 58312ExtracellularSequence analysisAdd
BLAST
Transmembranei584 – 60421HelicalSequence analysisAdd
BLAST
Topological domaini605 – 62218CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Polyamine transporter 3PRO_0000262731Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551PhosphoserineCombined sources
Modified residuei98 – 981PhosphothreonineCombined sources
Modified residuei101 – 1011PhosphoserineCombined sources
Modified residuei132 – 1321PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06451.

PTM databases

iPTMnetiQ06451.

Expressioni

Inductioni

By transcription factor HAA1 in response to acetaldehyde accumulation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi36323. 52 interactions.
DIPiDIP-3907N.
IntActiQ06451. 19 interactions.
MINTiMINT-560933.

Structurei

3D structure databases

ProteinModelPortaliQ06451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiQ06451.
OMAiGPIVCGW.
OrthoDBiEOG77M8XK.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06451-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRQESINSF NSDETSSLSD VESQQPQQYI PSESGSKSNM APNQLKLTRT
60 70 80 90 100
ETVKSLQDMG VSSKAPVPDV NAPQSSKNKI FPEEYTLETP TGLVPVATLH
110 120 130 140 150
SIGRTSTAIS RTRTRQIDGA SSPSSNEDAL ESDNNEKGKE GDSSGANDEA
160 170 180 190 200
PDLDPEIEFV TFVTGDPENP HNWPAWIRWS YTVLLSILVI CVAYGSACIS
210 220 230 240 250
GGLGTVEKKY HVGMEAAILS VSLMVIGFSL GPLIWSPVSD LYGRRVAYFV
260 270 280 290 300
SMGLYVIFNI PCALAPNLGS LLACRFLCGV WSSSGLCLVG GSIADMFPSE
310 320 330 340 350
TRGKAIAFFA FAPYVGPVVG PLVNGFISVS TGRMDLIFWV NMAFAGVMWI
360 370 380 390 400
ISSAIPETYA PVILKRKAAR LRKETGNPKI MTEQEAQGVS MGEMMRACLL
410 420 430 440 450
RPLYFSVTEP VLVATCFYVC LIYSLLYAFF FAFPVIFGEL YGYKDNLVGL
460 470 480 490 500
MFIPIVIGAL WALATTFYCE NKYLQIVKQR KPTPEDRLLG AKIGAPFAAI
510 520 530 540 550
ALWILGATAY KHIIWVGPAS AGLAFGFGMV LIYYSLNNYI IDCYVQYASS
560 570 580 590 600
ALATKVFLRS AGGAAFPLFT IQMYHKLNLH WGSWLLAFIS TAMIALPFAF
610 620
SYWGKGLRHK LSKKDYSIDS IE
Length:622
Mass (Da):68,066
Last modified:November 1, 1996 - v1
Checksum:iD260ED161F800502
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28371 Genomic DNA. Translation: AAB68053.1.
AY723870 Genomic DNA. Translation: AAU09787.1.
BK006949 Genomic DNA. Translation: DAA11568.1.
PIRiS61140.
RefSeqiNP_015482.1. NM_001184253.1.

Genome annotation databases

EnsemblFungiiYPR156C; YPR156C; YPR156C.
GeneIDi856279.
KEGGisce:YPR156C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28371 Genomic DNA. Translation: AAB68053.1.
AY723870 Genomic DNA. Translation: AAU09787.1.
BK006949 Genomic DNA. Translation: DAA11568.1.
PIRiS61140.
RefSeqiNP_015482.1. NM_001184253.1.

3D structure databases

ProteinModelPortaliQ06451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36323. 52 interactions.
DIPiDIP-3907N.
IntActiQ06451. 19 interactions.
MINTiMINT-560933.

Protein family/group databases

TCDBi2.A.1.2.66. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ06451.

Proteomic databases

MaxQBiQ06451.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR156C; YPR156C; YPR156C.
GeneIDi856279.
KEGGisce:YPR156C.

Organism-specific databases

EuPathDBiFungiDB:YPR156C.
SGDiS000006360. TPO3.

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiQ06451.
OMAiGPIVCGW.
OrthoDBiEOG77M8XK.

Enzyme and pathway databases

BioCyciYEAST:G3O-34287-MONOMER.

Miscellaneous databases

PROiQ06451.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Multiple polyamine transport systems on the vacuolar membrane in yeast."
    Tomitori H., Kashiwagi K., Asakawa T., Kakinuma Y., Michael A.J., Igarashi K.
    Biochem. J. 353:681-688(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Localization and function of the yeast multidrug transporter Tpo1p."
    Albertsen M., Bellahn I., Kraemer R., Waffenschmidt S.
    J. Biol. Chem. 278:12820-12825(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Exposure of Saccharomyces cerevisiae to acetaldehyde induces sulfur amino acid metabolism and polyamine transporter genes, which depend on Met4p and Haa1p transcription factors, respectively."
    Aranda A., del Olmo M.
    Appl. Environ. Microbiol. 70:1913-1922(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  9. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; THR-98; SER-101 AND SER-132, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTPO3_YEAST
AccessioniPrimary (citable) accession number: Q06451
Secondary accession number(s): D6W4F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2760 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.