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Protein

Polyamine transporter 3

Gene

TPO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermine, but not spermidine.2 Publications

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • spermine transmembrane transporter activity Source: SGD

GO - Biological processi

  • spermine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34287-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.66. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine transporter 3
Gene namesi
Name:TPO3
Ordered Locus Names:YPR156C
ORF Names:P9584.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR156C.
SGDiS000006360. TPO3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 182CytoplasmicSequence analysisAdd BLAST182
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 215ExtracellularSequence analysisAdd BLAST12
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Topological domaini237 – 245CytoplasmicSequence analysis9
Transmembranei246 – 266HelicalSequence analysisAdd BLAST21
Topological domaini267 – 275ExtracellularSequence analysis9
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Topological domaini297 – 305CytoplasmicSequence analysis9
Transmembranei306 – 326HelicalSequence analysisAdd BLAST21
Topological domaini327 – 335ExtracellularSequence analysis9
Transmembranei336 – 356HelicalSequence analysisAdd BLAST21
Topological domaini357 – 416CytoplasmicSequence analysisAdd BLAST60
Transmembranei417 – 437HelicalSequence analysisAdd BLAST21
Topological domaini438 – 446ExtracellularSequence analysis9
Transmembranei447 – 467HelicalSequence analysisAdd BLAST21
Topological domaini468 – 487CytoplasmicSequence analysisAdd BLAST20
Transmembranei488 – 508HelicalSequence analysisAdd BLAST21
Topological domaini509 – 512ExtracellularSequence analysis4
Transmembranei513 – 533HelicalSequence analysisAdd BLAST21
Topological domaini534 – 550CytoplasmicSequence analysisAdd BLAST17
Transmembranei551 – 571HelicalSequence analysisAdd BLAST21
Topological domaini572 – 583ExtracellularSequence analysisAdd BLAST12
Transmembranei584 – 604HelicalSequence analysisAdd BLAST21
Topological domaini605 – 622CytoplasmicSequence analysisAdd BLAST18

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002627311 – 622Polyamine transporter 3Add BLAST622

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55PhosphoserineCombined sources1
Modified residuei98PhosphothreonineCombined sources1
Modified residuei101PhosphoserineCombined sources1
Modified residuei132PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06451.
PRIDEiQ06451.

PTM databases

iPTMnetiQ06451.

Expressioni

Inductioni

By transcription factor HAA1 in response to acetaldehyde accumulation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi36323. 52 interactors.
DIPiDIP-3907N.
IntActiQ06451. 19 interactors.
MINTiMINT-560933.

Structurei

3D structure databases

ProteinModelPortaliQ06451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiQ06451.
OMAiVFPEEYT.
OrthoDBiEOG092C2079.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06451-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRQESINSF NSDETSSLSD VESQQPQQYI PSESGSKSNM APNQLKLTRT
60 70 80 90 100
ETVKSLQDMG VSSKAPVPDV NAPQSSKNKI FPEEYTLETP TGLVPVATLH
110 120 130 140 150
SIGRTSTAIS RTRTRQIDGA SSPSSNEDAL ESDNNEKGKE GDSSGANDEA
160 170 180 190 200
PDLDPEIEFV TFVTGDPENP HNWPAWIRWS YTVLLSILVI CVAYGSACIS
210 220 230 240 250
GGLGTVEKKY HVGMEAAILS VSLMVIGFSL GPLIWSPVSD LYGRRVAYFV
260 270 280 290 300
SMGLYVIFNI PCALAPNLGS LLACRFLCGV WSSSGLCLVG GSIADMFPSE
310 320 330 340 350
TRGKAIAFFA FAPYVGPVVG PLVNGFISVS TGRMDLIFWV NMAFAGVMWI
360 370 380 390 400
ISSAIPETYA PVILKRKAAR LRKETGNPKI MTEQEAQGVS MGEMMRACLL
410 420 430 440 450
RPLYFSVTEP VLVATCFYVC LIYSLLYAFF FAFPVIFGEL YGYKDNLVGL
460 470 480 490 500
MFIPIVIGAL WALATTFYCE NKYLQIVKQR KPTPEDRLLG AKIGAPFAAI
510 520 530 540 550
ALWILGATAY KHIIWVGPAS AGLAFGFGMV LIYYSLNNYI IDCYVQYASS
560 570 580 590 600
ALATKVFLRS AGGAAFPLFT IQMYHKLNLH WGSWLLAFIS TAMIALPFAF
610 620
SYWGKGLRHK LSKKDYSIDS IE
Length:622
Mass (Da):68,066
Last modified:November 1, 1996 - v1
Checksum:iD260ED161F800502
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28371 Genomic DNA. Translation: AAB68053.1.
AY723870 Genomic DNA. Translation: AAU09787.1.
BK006949 Genomic DNA. Translation: DAA11568.1.
PIRiS61140.
RefSeqiNP_015482.1. NM_001184253.1.

Genome annotation databases

EnsemblFungiiYPR156C; YPR156C; YPR156C.
GeneIDi856279.
KEGGisce:YPR156C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28371 Genomic DNA. Translation: AAB68053.1.
AY723870 Genomic DNA. Translation: AAU09787.1.
BK006949 Genomic DNA. Translation: DAA11568.1.
PIRiS61140.
RefSeqiNP_015482.1. NM_001184253.1.

3D structure databases

ProteinModelPortaliQ06451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36323. 52 interactors.
DIPiDIP-3907N.
IntActiQ06451. 19 interactors.
MINTiMINT-560933.

Protein family/group databases

TCDBi2.A.1.2.66. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ06451.

Proteomic databases

MaxQBiQ06451.
PRIDEiQ06451.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR156C; YPR156C; YPR156C.
GeneIDi856279.
KEGGisce:YPR156C.

Organism-specific databases

EuPathDBiFungiDB:YPR156C.
SGDiS000006360. TPO3.

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiQ06451.
OMAiVFPEEYT.
OrthoDBiEOG092C2079.

Enzyme and pathway databases

BioCyciYEAST:G3O-34287-MONOMER.

Miscellaneous databases

PROiQ06451.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPO3_YEAST
AccessioniPrimary (citable) accession number: Q06451
Secondary accession number(s): D6W4F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2760 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.