Q06442 (WNT5A_AMBME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-5a

Gene names

Name:

WNT-5A

Organism

Ambystoma mexicanum (Axolotl)

Taxonomic identifier

8296 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Caudata › Salamandroidea › Ambystomatidae › Ambystoma

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Neuroectodermal and non-neuroectodermal tissues.
Developmental stage	Abundant in the blastula until gastrulation, barely detectable during gastrulation, and increase again during neurulation. Detected throughout the remaining development and in hatched larvae.
Post-translational modification	Palmitoylation at Ser-223 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-83. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 359	339	Protein Wnt-5a		PRO_0000041432
Amino acid modifications
	Lipidation	83	1	S-palmitoyl cysteine By similarity
	Lipidation	223	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	291	1	N-linked (GlcNAc...) Potential
	Glycosylation	305	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q06442 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 533FFB0414DAAA14	FASTA	359	40,117
	10 20 30 40 50 60 MATTHLTAAL ALLCALLQVD IEASSWWSLA MNPVQIPEAY IVGAQPLCSQ LPGLSPGQKK 70 80 90 100 110 120 LCQLYQDHMP YIGEGAKTGI KECQYQFRHR RWNCSTVDNA SVFGRVMQIG SRETAFTYSI 130 140 150 160 170 180 SAAGVVNAVS RACREGELST CGCSRAARPK DLQRDWLWGG CGDNLEYGYR FAKEFVDARE 190 200 210 220 230 240 REKIHTKGSY ESSRTLMNIH NNEAGRRTVY NLADAACKCH GVSGSCSLKT CWLQLADFRK 250 260 270 280 290 300 VGDFLKEKYD SAASMRLNAR GKLVQVNSRF NPPTTNDLVY VDTSPDYCVR NESTGSLGTQ 310 320 330 340 350 GRLCNKTSEG MDGCELMCCG RGYDQFKTVQ TERCHCKFHW CCYVKCKKCT EIVDQFVCK

References

[1]	"Isolation of cDNAs for two closely related members of the axolotl Wnt family, Awnt-5A and Awnt-5B, and analysis of their expression during development." Busse U., Seguin C. Mech. Dev. 40:63-72(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z14047 mRNA. Translation: CAA78415.1.
PIR	A56549.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG001595.
Family and domain databases
InterPro	IPR005817. Wnt. IPR026538. Wnt5a. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF33. PTHR12027:SF33. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WNT5A_AMBME

Accession

Primary (citable) accession number: Q06442

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program