ID   ODPAT_RAT               Reviewed;         391 AA.
AC   Q06437;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   16-JUN-2009, entry version 69.
DE   RecName: Full=Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial;
DE            EC=1.2.4.1;
DE   AltName: Full=PDHE1-A type II;
DE   Flags: Precursor;
GN   Name=Pdha2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Testis;
RX   MEDLINE=94032478; PubMed=7916643; DOI=10.1016/0167-4781(93)90054-H;
RA   Cullingford T.E., Clark J.B., Phillips I.R.;
RT   "Characterization of cDNAs encoding the rat testis-specific E1 alpha
RT   subunit of the pyruvate dehydrogenase complex: comparison of
RT   expression of the corresponding mRNA with that of the somatic E1 alpha
RT   subunit.";
RL   Biochim. Biophys. Acta 1216:149-153(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Testis;
RX   MEDLINE=99004058; PubMed=9787790; DOI=10.1016/S0305-0491(98)10010-X;
RA   Jeng J., Kallarakal A.T., Kim S.F., Popov K.M., Song B.J.;
RT   "Pyruvate dehydrogenase E1 alpha isoform in rat testis: cDNA cloning,
RT   characterization, and biochemical comparison of the recombinant testis
RT   and liver enzymes.";
RL   Comp. Biochem. Physiol. 120B:205-216(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: The pyruvate dehydrogenase complex catalyzes the overall
CC       conversion of pyruvate to acetyl-CoA and CO(2). It contains
CC       multiple copies of three enzymatic components: pyruvate
CC       dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and
CC       lipoamide dehydrogenase (E3).
CC   -!- CATALYTIC ACTIVITY: Pyruvate + [dihydrolipoyllysine-residue
CC       acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue
CC       acetyltransferase] S-acetyldihydrolipoyllysine + CO(2).
CC   -!- COFACTOR: Thiamine pyrophosphate.
CC   -!- ENZYME REGULATION: E1 activity is regulated by phosphorylation
CC       (inactivation) and dephosphorylation (activation) of the alpha
CC       subunit.
CC   -!- SUBUNIT: Tetramer of 2 alpha and 2 beta subunits.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion (Probable).
CC   -!- TISSUE SPECIFICITY: Testis.
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DR   EMBL; Z18878; CAA79318.1; -; mRNA.
DR   EMBL; U44125; AAB68458.1; -; mRNA.
DR   EMBL; BC078757; AAH78757.1; -; mRNA.
DR   IPI; IPI00193263; -.
DR   PIR; S31416; S31416.
DR   RefSeq; NP_446446.1; -.
DR   UniGene; Rn.11126; -.
DR   HSSP; P08559; 1NI4.
DR   SMR; Q06437; 31-391.
DR   Ensembl; ENSRNOG00000016223; Rattus norvegicus.
DR   GeneID; 117098; -.
DR   KEGG; rno:117098; -.
DR   NMPDR; fig|10116.3.peg.17098; -.
DR   RGD; 620095; Pdha2.
DR   HOVERGEN; Q06437; -.
DR   OMA; Q06437; NDATCDI.
DR   BRENDA; 1.2.4.1; 248.
DR   NextBio; 619976; -.
DR   ArrayExpress; Q06437; -.
DR   GermOnline; ENSRNOG00000016223; Rattus norvegicus.
DR   GO; GO:0005739; C:mitochondrion; IDA:RGD.
DR   GO; GO:0045254; C:pyruvate dehydrogenase complex; IDA:RGD.
DR   GO; GO:0004739; F:pyruvate dehydrogenase (acetyl-transferring...; IDA:RGD.
DR   GO; GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:RGD.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001017; DH_E1.
DR   InterPro; IPR017597; Pyrv_DH_E1_asu_subgrp-y.
DR   Pfam; PF00676; E1_dh; 1.
DR   TIGRFAMs; TIGR03182; PDH_E1_alph_y; 1.
PE   2: Evidence at transcript level;
KW   Glycolysis; Mitochondrion; Oxidoreductase; Phosphoprotein; Pyruvate;
KW   Thiamine pyrophosphate; Transit peptide.
FT   TRANSIT       1     30       Mitochondrion (By similarity).
FT   CHAIN        31    391       Pyruvate dehydrogenase E1 component
FT                                subunit alpha, testis-specific form,
FT                                mitochondrial.
FT                                /FTId=PRO_0000020449.
FT   MOD_RES     290    290       Phosphotyrosine (By similarity).
FT   MOD_RES     294    294       Phosphoserine (By similarity).
FT   MOD_RES     301    301       Phosphoserine (By similarity).
FT   MOD_RES     302    302       Phosphotyrosine (By similarity).
SQ   SEQUENCE   391 AA;  43393 MW;  5BF049BEE483EF5D CRC64;
     MRKMLATVLS QVFSGMVQKP ALRGLLSSLK FSNDATCDIK KCDLYLLEQG PPTSTVLTRE
     EALKYYRNMQ VIRRMELKAD QLYKQKFIRG FCHLCDGQEA CNVGLEAGIN PTDHIITSYR
     AHGLCYTRGL SVKSILAELT GRKGGCAKGK GGSMHMYAKN FYGGNGIVGA QVPLGAGVAL
     ACKYLKNGQI CLALYGDGAA NQGQVFEAYN MSALWKLPCV FICENNRYGM GTAIERSAAS
     TDYHKKGFVI PGLRVNGMDI LSVREATKFA ADHCRSGKGP IVMELQTYRY HGHSMSDPGI
     SYRTREEVQN VRSKSDPIML LRERMISNNL SSVEELKEID ADVKKEVEEA AQFATTDPEP
     PLEDLANYLY HQNPPFEVRG AHKWLKFKSV S
//