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Q06437 (ODPAT_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial

EC=1.2.4.1
Alternative name(s):
PDHE1-A type II
Gene names
Name:Pdha2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle. Ref.2

Catalytic activity

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2. Ref.2

Cofactor

Thiamine pyrophosphate By similarity.

Enzyme regulation

Pyruvate dehydrogenase activity is inhibited by phosphorylation of PDHA2; it is reactivated by dephosphorylation By similarity.

Subunit structure

Heterotetramer of two PDHA2 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules By similarity.

Subcellular location

Mitochondrion matrix By similarity.

Tissue specificity

Testis. Ref.2

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3030Mitochondrion By similarity
Chain31 – 391361Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
PRO_0000020449

Amino acid modifications

Modified residue2941Phosphoserine; by PDK1, PDK2, PDK3 and PDK4 By similarity
Modified residue3011Phosphoserine; by PDK3 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q06437 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 5BF049BEE483EF5D

FASTA39143,393
        10         20         30         40         50         60 
MRKMLATVLS QVFSGMVQKP ALRGLLSSLK FSNDATCDIK KCDLYLLEQG PPTSTVLTRE 

        70         80         90        100        110        120 
EALKYYRNMQ VIRRMELKAD QLYKQKFIRG FCHLCDGQEA CNVGLEAGIN PTDHIITSYR 

       130        140        150        160        170        180 
AHGLCYTRGL SVKSILAELT GRKGGCAKGK GGSMHMYAKN FYGGNGIVGA QVPLGAGVAL 

       190        200        210        220        230        240 
ACKYLKNGQI CLALYGDGAA NQGQVFEAYN MSALWKLPCV FICENNRYGM GTAIERSAAS 

       250        260        270        280        290        300 
TDYHKKGFVI PGLRVNGMDI LSVREATKFA ADHCRSGKGP IVMELQTYRY HGHSMSDPGI 

       310        320        330        340        350        360 
SYRTREEVQN VRSKSDPIML LRERMISNNL SSVEELKEID ADVKKEVEEA AQFATTDPEP 

       370        380        390 
PLEDLANYLY HQNPPFEVRG AHKWLKFKSV S 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of cDNAs encoding the rat testis-specific E1 alpha subunit of the pyruvate dehydrogenase complex: comparison of expression of the corresponding mRNA with that of the somatic E1 alpha subunit."
Cullingford T.E., Clark J.B., Phillips I.R.
Biochim. Biophys. Acta 1216:149-153(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[2]"Pyruvate dehydrogenase E1 alpha isoform in rat testis: cDNA cloning, characterization, and biochemical comparison of the recombinant testis and liver enzymes."
Jeng J., Kallarakal A.T., Kim S.F., Popov K.M., Song B.J.
Comp. Biochem. Physiol. 120B:205-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION, PHOSPHORYLATION, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z18878 mRNA. Translation: CAA79318.1.
U44125 mRNA. Translation: AAB68458.1.
BC078757 mRNA. Translation: AAH78757.1.
PIRS31416.
RefSeqNP_446446.1. NM_053994.2.
UniGeneRn.11126.

3D structure databases

ProteinModelPortalQ06437.
SMRQ06437. Positions 31-391.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ06437.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID117098.
KEGGrno:117098.

Organism-specific databases

CTD5161.
RGD620095. Pdha2.

Phylogenomic databases

HOVERGENHBG001863.
InParanoidQ06437.
KOK00161.
PhylomeDBQ06437.

Gene expression databases

GenevestigatorQ06437.

Family and domain databases

Gene3D3.40.50.970. 1 hit.
InterProIPR001017. DH_E1.
IPR017597. Pyrv_DH_E1_asu_subgrp-y.
IPR029061. THDP-binding.
[Graphical view]
PfamPF00676. E1_dh. 1 hit.
[Graphical view]
SUPFAMSSF52518. SSF52518. 1 hit.
TIGRFAMsTIGR03182. PDH_E1_alph_y. 1 hit.
ProtoNetSearch...

Other

NextBio619976.
PROQ06437.

Entry information

Entry nameODPAT_RAT
AccessionPrimary (citable) accession number: Q06437
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program