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Q06437

- ODPAT_RAT

UniProt

Q06437 - ODPAT_RAT

Protein

Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial

Gene

Pdha2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.1 Publication

    Catalytic activityi

    Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.1 Publication

    Cofactori

    Thiamine pyrophosphate.By similarity

    Enzyme regulationi

    Pyruvate dehydrogenase activity is inhibited by phosphorylation of PDHA2; it is reactivated by dephosphorylation.By similarity

    GO - Molecular functioni

    1. pyruvate dehydrogenase (acetyl-transferring) activity Source: RGD

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from pyruvate Source: RGD
    2. glucose metabolic process Source: UniProtKB-KW
    3. glycolytic process Source: InterPro
    4. pyruvate metabolic process Source: UniProtKB
    5. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism, Tricarboxylic acid cycle

    Keywords - Ligandi

    Pyruvate, Thiamine pyrophosphate

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial (EC:1.2.4.1)
    Alternative name(s):
    PDHE1-A type II
    Gene namesi
    Name:Pdha2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620095. Pdha2.

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: RGD
    3. pyruvate dehydrogenase complex Source: RGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3030MitochondrionBy similarityAdd
    BLAST
    Chaini31 – 391361Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrialPRO_0000020449Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei294 – 2941Phosphoserine; by PDK1, PDK2, PDK3 and PDK4By similarity
    Modified residuei301 – 3011Phosphoserine; by PDK3By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ06437.

    Expressioni

    Tissue specificityi

    Testis.1 Publication

    Gene expression databases

    GenevestigatoriQ06437.

    Interactioni

    Subunit structurei

    Heterotetramer of two PDHA2 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ06437.
    SMRiQ06437. Positions 31-391.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOVERGENiHBG001863.
    InParanoidiQ06437.
    KOiK00161.
    PhylomeDBiQ06437.

    Family and domain databases

    Gene3Di3.40.50.970. 1 hit.
    InterProiIPR001017. DH_E1.
    IPR017597. Pyrv_DH_E1_asu_subgrp-y.
    IPR029061. THDP-binding.
    [Graphical view]
    PfamiPF00676. E1_dh. 1 hit.
    [Graphical view]
    SUPFAMiSSF52518. SSF52518. 1 hit.
    TIGRFAMsiTIGR03182. PDH_E1_alph_y. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q06437-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKMLATVLS QVFSGMVQKP ALRGLLSSLK FSNDATCDIK KCDLYLLEQG    50
    PPTSTVLTRE EALKYYRNMQ VIRRMELKAD QLYKQKFIRG FCHLCDGQEA 100
    CNVGLEAGIN PTDHIITSYR AHGLCYTRGL SVKSILAELT GRKGGCAKGK 150
    GGSMHMYAKN FYGGNGIVGA QVPLGAGVAL ACKYLKNGQI CLALYGDGAA 200
    NQGQVFEAYN MSALWKLPCV FICENNRYGM GTAIERSAAS TDYHKKGFVI 250
    PGLRVNGMDI LSVREATKFA ADHCRSGKGP IVMELQTYRY HGHSMSDPGI 300
    SYRTREEVQN VRSKSDPIML LRERMISNNL SSVEELKEID ADVKKEVEEA 350
    AQFATTDPEP PLEDLANYLY HQNPPFEVRG AHKWLKFKSV S 391
    Length:391
    Mass (Da):43,393
    Last modified:June 1, 1994 - v1
    Checksum:i5BF049BEE483EF5D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z18878 mRNA. Translation: CAA79318.1.
    U44125 mRNA. Translation: AAB68458.1.
    BC078757 mRNA. Translation: AAH78757.1.
    PIRiS31416.
    RefSeqiNP_446446.1. NM_053994.2.
    UniGeneiRn.11126.

    Genome annotation databases

    GeneIDi117098.
    KEGGirno:117098.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z18878 mRNA. Translation: CAA79318.1 .
    U44125 mRNA. Translation: AAB68458.1 .
    BC078757 mRNA. Translation: AAH78757.1 .
    PIRi S31416.
    RefSeqi NP_446446.1. NM_053994.2.
    UniGenei Rn.11126.

    3D structure databases

    ProteinModelPortali Q06437.
    SMRi Q06437. Positions 31-391.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q06437.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 117098.
    KEGGi rno:117098.

    Organism-specific databases

    CTDi 5161.
    RGDi 620095. Pdha2.

    Phylogenomic databases

    HOVERGENi HBG001863.
    InParanoidi Q06437.
    KOi K00161.
    PhylomeDBi Q06437.

    Miscellaneous databases

    NextBioi 619976.
    PROi Q06437.

    Gene expression databases

    Genevestigatori Q06437.

    Family and domain databases

    Gene3Di 3.40.50.970. 1 hit.
    InterProi IPR001017. DH_E1.
    IPR017597. Pyrv_DH_E1_asu_subgrp-y.
    IPR029061. THDP-binding.
    [Graphical view ]
    Pfami PF00676. E1_dh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52518. SSF52518. 1 hit.
    TIGRFAMsi TIGR03182. PDH_E1_alph_y. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of cDNAs encoding the rat testis-specific E1 alpha subunit of the pyruvate dehydrogenase complex: comparison of expression of the corresponding mRNA with that of the somatic E1 alpha subunit."
      Cullingford T.E., Clark J.B., Phillips I.R.
      Biochim. Biophys. Acta 1216:149-153(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    2. "Pyruvate dehydrogenase E1 alpha isoform in rat testis: cDNA cloning, characterization, and biochemical comparison of the recombinant testis and liver enzymes."
      Jeng J., Kallarakal A.T., Kim S.F., Popov K.M., Song B.J.
      Comp. Biochem. Physiol. 120B:205-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION, PHOSPHORYLATION, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiODPAT_RAT
    AccessioniPrimary (citable) accession number: Q06437
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    External Data

    Dasty 3