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Protein

Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial

Gene

Pdha2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.1 Publication

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.1 Publication

Cofactori

Enzyme regulationi

Pyruvate dehydrogenase activity is inhibited by phosphorylation of PDHA2; it is reactivated by dephosphorylation.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism, Tricarboxylic acid cycle
LigandPyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

ReactomeiR-RNO-204174. Regulation of pyruvate dehydrogenase (PDH) complex.
R-RNO-389661. Glyoxylate metabolism and glycine degradation.
R-RNO-5362517. Signaling by Retinoic Acid.
R-RNO-70268. Pyruvate metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial (EC:1.2.4.1)
Alternative name(s):
PDHE1-A type II
Gene namesi
Name:Pdha2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi620095. Pdha2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30MitochondrionBy similarityAdd BLAST30
ChainiPRO_000002044931 – 391Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrialAdd BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei294PhosphoserineCombined sources1
Modified residuei301Phosphoserine; by PDK3By similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ06437.

PTM databases

iPTMnetiQ06437.
PhosphoSitePlusiQ06437.

Expressioni

Tissue specificityi

Testis.1 Publication

Gene expression databases

BgeeiENSRNOG00000016223.
GenevisibleiQ06437. RN.

Interactioni

Subunit structurei

Heterotetramer of two PDHA2 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ06437.
SMRiQ06437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00530000063174.
HOVERGENiHBG001863.
InParanoidiQ06437.
KOiK00161.
OMAiAYNMPGV.
OrthoDBiEOG091G0966.
PhylomeDBiQ06437.

Family and domain databases

InterProiView protein in InterPro
IPR001017. DH_E1.
IPR017597. Pyrv_DH_E1_asu_subgrp-y.
IPR029061. THDP-binding.
PfamiView protein in Pfam
PF00676. E1_dh. 1 hit.
SUPFAMiSSF52518. SSF52518. 1 hit.
TIGRFAMsiTIGR03182. PDH_E1_alph_y. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKMLATVLS QVFSGMVQKP ALRGLLSSLK FSNDATCDIK KCDLYLLEQG
60 70 80 90 100
PPTSTVLTRE EALKYYRNMQ VIRRMELKAD QLYKQKFIRG FCHLCDGQEA
110 120 130 140 150
CNVGLEAGIN PTDHIITSYR AHGLCYTRGL SVKSILAELT GRKGGCAKGK
160 170 180 190 200
GGSMHMYAKN FYGGNGIVGA QVPLGAGVAL ACKYLKNGQI CLALYGDGAA
210 220 230 240 250
NQGQVFEAYN MSALWKLPCV FICENNRYGM GTAIERSAAS TDYHKKGFVI
260 270 280 290 300
PGLRVNGMDI LSVREATKFA ADHCRSGKGP IVMELQTYRY HGHSMSDPGI
310 320 330 340 350
SYRTREEVQN VRSKSDPIML LRERMISNNL SSVEELKEID ADVKKEVEEA
360 370 380 390
AQFATTDPEP PLEDLANYLY HQNPPFEVRG AHKWLKFKSV S
Length:391
Mass (Da):43,393
Last modified:June 1, 1994 - v1
Checksum:i5BF049BEE483EF5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18878 mRNA. Translation: CAA79318.1.
U44125 mRNA. Translation: AAB68458.1.
BC078757 mRNA. Translation: AAH78757.1.
PIRiS31416.
RefSeqiNP_446446.1. NM_053994.2.
UniGeneiRn.11126.

Genome annotation databases

EnsembliENSRNOT00000021719; ENSRNOP00000071767; ENSRNOG00000016223.
GeneIDi117098.
KEGGirno:117098.

Similar proteinsi

Entry informationi

Entry nameiODPAT_RAT
AccessioniPrimary (citable) accession number: Q06437
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 22, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome