Reviewed, UniProtKB/Swiss-Prot Q06434 (GLTB_ANTSP)

Names and origin

Protein names

Recommended name:
    Ferredoxin-dependent glutamate synthase
    EC=1.4.7.1
Alternative name(s):
    Fd-GOGAT

Gene names

Name:	gltB
Synonyms:	glsF

Encoded on

Plastid; Chloroplast

Organism

Antithamnion sp.

Taxonomic identifier

2767 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Florideophyceae › Ceramiales › Ceramiaceae › Antithamnion

Protein attributes

Sequence length	1536 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+.
Cofactor	Binds 1 3Fe-4S cluster. FAD. FMN.
Pathway	Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1. Energy metabolism; nitrogen metabolism.
Subunit structure	Monomer.
Subcellular location	Plastid › chloroplast stroma.
Sequence similarities	Belongs to the glutamate synthase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Glutamate biosynthesis
Cellular component	Chloroplast Plastid
Domain	Glutamine amidotransferase
Ligand	3Fe-4S FAD FMN Flavoprotein Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate synthase (ferredoxin) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1536	1536	Ferredoxin-dependent glutamate synthase		PRO_0000170790
Regions
	Domain	27 – 427	401	Glutamine amidotransferase type-2
	Nucleotide binding	1105 – 1162	58	FMN By similarity
Sites
	Active site	27	1	For GATase activity By similarity
	Metal binding	1158	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	1164	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	1169	1	Iron-sulfur (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06434-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: E803CED3004F321C	FASTA	1,536	171,112
	10 20 30 40 50 60 MQVSKYFTHQ LSQFSGYPSI VSERDACGVG FIANLNSKPS NKIVTEALNA LSCMEHRGGC 70 80 90 100 110 120 GADNISGDGA GVTIQIPWDI FISEGINFLP KLQSNQSILN YGVRMILRSS DDLDKIKKIF 130 140 150 160 170 180 SWALDEYQLD LISWRNVPVD KSILGEESKF NQPLVVQCIV RSNNLIDYKL DKHLYLVRKK 190 200 210 220 230 240 IEKLVSKLDI NTNKQFYICS FSSKTIVYKG MLRSEFLVKY YNDLSNSLYV SNFAMYHRRF 250 260 270 280 290 300 STNTMPKWSL AQPMRFMAHN GEINTLLGNL NWNKSKESLL KSSIWSDYYD ILSPITNLEN 310 320 330 340 350 360 SDSANLDSVL ELFIHSGRTP QEALMILIPE AYKNQPALSL FPEITDFYEY YSILQEPWDG 370 380 390 400 410 420 PALVVFTDGK FVGATLDRNG LRPARYTITD DGFISLSSET GVSNINSQNV VTKGRLGPGQ 430 440 450 460 470 480 MLCVDLSKNL VLDNWMIKQQ ISQKFPYKEW VNKYQSNLNL LEYLNDFTFD KVQMNRWHTA 490 500 510 520 530 540 FGYTNEDVEL VIEHMASSAK EPTFSMGDDT PLPILSEKPH LIYDYFKQRF AQVTNPAIDP 550 560 570 580 590 600 LRESLVMSLI TYLGPKGNIL EPTAIMAKSI KLESPIINEN ELAQLNSFNL SVVTVPTFID 610 620 630 640 650 660 KHLSTQTFVD KILEICSQCD SYISQGIEIL VLSDRIEILP VDKIFVSPLL IVGAVHHYLI 670 680 690 700 710 720 KKQLRHKVSL VIDTGQCWTT HHFALLIGYG ASAICPYLAF LTVRQWWHNS RTQKLMSTGK 730 740 750 760 770 780 LSRLTIQESQ DNYRSAIEKG LLKILSKMGI SLLSSYHGAQ IFEILGLGQD VVDLAFSGTV 790 800 810 820 830 840 SRLNGMTLNE LYEDSLKSYN LAFITEIPKK LPNLGYVQYR PSAEYHVNNP EMSKTLHKAV 850 860 870 880 890 900 RNNDNILYSK YKSLLNDRRP TNLRDLLELK TDRQPISIDQ VEDVNSVLMR FCTGGMSLGA 910 920 930 940 950 960 LSRETHETLA IRMNRIGGKS NSGEGGEDST RFKSIQDLDT SGVSRTFSHL KGLKINDLAS 970 980 990 1000 1010 1020 SAIKQIASGR FGVTPEYLVN AKQLEIKIAQ GAKPGEGGQL PGKKVSPYIA ELRNCKPGVT 1030 1040 1050 1060 1070 1080 LISPPPHHDI YSIEDLAQLI FDLHQINPDA QVSVKLVASL GIGTIAAGVA KGNADIIQIS 1090 1100 1110 1120 1130 1140 GHDGGTGASP LSSIKHAGAP WDVGLAEVHT TLVENSLREK VILRVDGGLR TGKDIIIAAL 1150 1160 1170 1180 1190 1200 MGAEEFGFGT VAMIATGCVM ARVCHTNNCP VGVATQRQDL RNRFPGIPSD VVNFFIFVAE 1210 1220 1230 1240 1250 1260 EVREILAELG YKSLEELIGL NDLFKVKDIE LSKTKNLNLN ILFNSINMNR NLIPKLKHKT 1270 1280 1290 1300 1310 1320 VHTNGNVLDD ILLSKSNIIN AINLQSNIVQ DIEILNTDRC VGARISGLIT KMYGRDNFNG 1330 1340 1350 1360 1370 1380 NLQLNFVGSA GQSFGAFISK GIHLYLKGEA NDYVGKGMNG GEIIICPPIE QKTSSSNQVI 1390 1400 1410 1420 1430 1440 LGNTCLYGAT GGYLFANGQA GERFAVRNSN GYSVVEGVGD HACEYMTGGL IVVLGTFGRN 1450 1460 1470 1480 1490 1500 IGAGMTGGIA YFLDEDNTLK NKLNTEIVKA QRLLTKESEE QLKNIMELYE IKTKSEKAKL 1510 1520 1530 ILDNWSQYLA KFYQIVPPSE QIQHLLMLIF FFSKYC

References

[1]	"Glutamate synthase is plastid-encoded in a red alga: implications for the evolution of glutamate synthases." Valentin K.-U., Kostrzewa M., Zetsche K. Plant Mol. Biol. 23:77-85(1993) [PubMed: 8219058] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z21705 Genomic DNA. Translation: CAA79809.1.
PIR	S39510.
3D structure databases
HSSP	HSSP built from PDB template 1OFD based on UniProtKB P55038.
ModBase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000583. GATase_2. IPR017932. GATase_II. IPR002932. Glu_synth_centr_C. IPR006982. Glu_synth_centr_N. IPR002489. Glu_synthase_C. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 2 hits. G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF04898. Glu_syn_central. 1 hit. PF01645. Glu_synthase. 1 hit. PF01493. GXGXG. 1 hit. [Graphical view]
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLTB_ANTSP

Accession

Primary (citable) accession number: Q06434

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)