Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q06412 (TUS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rho1 guanine nucleotide exchange factor TUS1
Alternative name(s):
TOR unique function suppressor protein 1
Gene names
Name:TUS1
Synonyms:SOP10
Ordered Locus Names:YLR425W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1307 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide-exchange factor (GEF) for RHO1 that stimulates the exchange of RHO1 GDP-bound form into GTP-bound form. Required for signaling of cell wall defects to RHO1. Ref.3

Subunit structure

Interacts with RHO1. Ref.3

Domain

The DH domain mediates interaction with RHO1.

Sequence similarities

Contains 1 CNH domain.

Contains 1 DH (DBL-homology) domain.

Contains 1 PH domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SHO1P400733EBI-37117,EBI-18140

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13071307Rho1 guanine nucleotide exchange factor TUS1
PRO_0000080979

Regions

Domain467 – 657191DH
Domain715 – 877163PH
Domain938 – 1279342CNH
Compositional bias105 – 1106Poly-Pro
Compositional bias227 – 2304Poly-Pro

Sequences

Sequence LengthMass (Da)Tools
Q06412 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 561574AB58FFBC54

FASTA1,307149,113
        10         20         30         40         50         60 
MYRYNRSSPF ERTPEKRVSR QESQRKSIEL PKLPPLNTRN SFLDDSDNGT DNISIGWTPI 

        70         80         90        100        110        120 
SDTQQFQSPV PQAFTFTSKH SARGNGTSSS ESTPKSTKYV KERRPPPPPP LLYSTESIRI 

       130        140        150        160        170        180 
DSPMVSPSSQ SRERSPNKLS FIGNSEERHH MEYISNHSRI LKSPFANGFS PNSPKSPRDS 

       190        200        210        220        230        240 
SKQQAHFSDE SDLRCHEREK ALPPIPFTTT LLLSPFDDED SEFFTKPPPP LSTSRNVSGN 

       250        260        270        280        290        300 
SRVSEALESV YSDSDYTFNN SNARQSSFNS LLGAKPLELA PSITAPTQPF SIQSIDEHKL 

       310        320        330        340        350        360 
YQCDNVYKLS AIYEWILKVY FEWFNECVFT KIDLFQIVQL LLEFQMPTNF DQDTIDSNVD 

       370        380        390        400        410        420 
NIMASFISQK AVRFDIINDE EVAVVVGGLD ITGVFTELLP CYSFIDNTYG STNSLICYSN 

       430        440        450        460        470        480 
VCTHGQSSGF RKEIKLSEII NKSVGLWTEY WHLTPDDLAE INPREVQRQS FIFDLIILEE 

       490        500        510        520        530        540 
RSLNMATAAV EIYGKRFDKS LLPDEPEFKA LAFDIFEPLI QLHTEFLLTP IFWKLKTRGK 

       550        560        570        580        590        600 
FIDGVGKIYS KWCGEAKNIY LNYAKAMATV HEIIMWEKKN KTKFVTWLKE IDNSVEITRS 

       610        620        630        640        650        660 
KMYHDVIFFG GFFKSLQNMP VTLRSILKNT DPSMEDYEYL KIVIKEVEKL NFEVNQVHGL 

       670        680        690        700        710        720 
AIDHRKLVRF SKQLVLSTNS SNATSYVNVG GSTNANDDDA IQDKLALGLT YPERKLVLSG 

       730        740        750        760        770        780 
TVYKKRDLWL DPTPVYIALL DNCLLITEEI SKGETQKYKL IERPIPIDYL SLEKRKIPGT 

       790        800        810        820        830        840 
SKQPLRNYSQ KEHKSPMHNF STPINSMRPL LKSSGNHMST AYGDRKTSNT EISNANPNTD 

       850        860        870        880        890        900 
EFSFKIRNTA TGESFKFFTE SAEVLNQWID AIMESFKRNA ENHDLNAFEF TVLSSEFAYF 

       910        920        930        940        950        960 
DKDAPVNLPV APEGSEIDVA LKAYAQKANK DSCSWSKTTR ILCCEDVKFE GRIYLFVATT 

       970        980        990       1000       1010       1020 
DGIYVKYRDD YGSGFVKILE LNDVKRMEAN VKLGLLFVLD NRKLCYFNIS TVVSRYLAQG 

      1030       1040       1050       1060       1070       1080 
NTLDENCIVG TVIRDKVRFF KIADDFGNSK HLFFERKGKI VILTPEFDQL TNQVKYFKFY 

      1090       1100       1110       1120       1130       1140 
KEYKLPSSSN NILNNEIEDI AIFRKSFAVC TKKTVILYQD SFEDNGIVLP SFLNDKDMMA 

      1150       1160       1170       1180       1190       1200 
HLRHPHLNSL PFKSATDSKK RPSIESLTEE AKKDIATCKA IPVNFFQISQ SSFFALVYDE 

      1210       1220       1230       1240       1250       1260 
AVVKINCYGE MSDWRKDILL LDFCCTGASF HGNHLILVGD NLIQIYDLKN VEQNLGELVP 

      1270       1280       1290       1300 
VQIIKGKKIK LASSERREKT ILVLSHPNIL NRQLLVACNP VAMADHQ 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast."
Schmelzle T., Helliwell S.B., Hall M.N.
Mol. Cell. Biol. 22:1329-1339(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RHO1.
[4]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20939 Genomic DNA. Translation: AAB67506.1.
BK006945 Genomic DNA. Translation: DAA09727.1.
PIRS53412.
RefSeqNP_013529.3. NM_001182313.3.

3D structure databases

ProteinModelPortalQ06412.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31685. 71 interactions.
DIPDIP-4635N.
IntActQ06412. 4 interactions.
MINTMINT-486172.
STRING4932.YLR425W.

Proteomic databases

PaxDbQ06412.
PeptideAtlasQ06412.
PRIDEQ06412.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR425W; YLR425W; YLR425W.
GeneID851145.
KEGGsce:YLR425W.

Organism-specific databases

CYGDYLR425w.
SGDS000004417. TUS1.

Phylogenomic databases

eggNOGNOG238720.
HOGENOMHOG000141939.
OMAAAVEIYG.
OrthoDBEOG7SXWBK.

Enzyme and pathway databases

BioCycYEAST:G3O-32485-MONOMER.

Gene expression databases

GenevestigatorQ06412.

Family and domain databases

Gene3D1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProIPR001180. Citron.
IPR000219. DH-domain.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PfamPF00780. CNH. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTSM00036. CNH. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMSSF48065. SSF48065. 1 hit.
PROSITEPS50219. CNH. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio967912.

Entry information

Entry nameTUS1_YEAST
AccessionPrimary (citable) accession number: Q06412
Secondary accession number(s): D6VZ61
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families