Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q06412

- TUS1_YEAST

UniProt

Q06412 - TUS1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Rho1 guanine nucleotide exchange factor TUS1

Gene

TUS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Guanine nucleotide-exchange factor (GEF) for RHO1 that stimulates the exchange of RHO1 GDP-bound form into GTP-bound form. Required for signaling of cell wall defects to RHO1.1 Publication

GO - Molecular functioni

  1. Rho guanyl-nucleotide exchange factor activity Source: SGD
  2. small GTPase regulator activity Source: InterPro

GO - Biological processi

  1. fungal-type cell wall organization Source: SGD
  2. positive regulation of Rho GTPase activity Source: GOC
  3. signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciYEAST:G3O-32485-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho1 guanine nucleotide exchange factor TUS1
Alternative name(s):
TOR unique function suppressor protein 1
Gene namesi
Name:TUS1
Synonyms:SOP10
Ordered Locus Names:YLR425W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR425w.
SGDiS000004417. TUS1.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. incipient cellular bud site Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13071307Rho1 guanine nucleotide exchange factor TUS1PRO_0000080979Add
BLAST

Proteomic databases

MaxQBiQ06412.
PaxDbiQ06412.
PeptideAtlasiQ06412.
PRIDEiQ06412.

Expressioni

Gene expression databases

GenevestigatoriQ06412.

Interactioni

Subunit structurei

Interacts with RHO1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SHO1P400733EBI-37117,EBI-18140

Protein-protein interaction databases

BioGridi31685. 72 interactions.
DIPiDIP-4635N.
IntActiQ06412. 4 interactions.
MINTiMINT-486172.
STRINGi4932.YLR425W.

Structurei

3D structure databases

ProteinModelPortaliQ06412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini467 – 657191DHPROSITE-ProRule annotationAdd
BLAST
Domaini715 – 877163PHPROSITE-ProRule annotationAdd
BLAST
Domaini938 – 1279342CNHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi105 – 1106Poly-Pro
Compositional biasi227 – 2304Poly-Pro

Domaini

The DH domain mediates interaction with RHO1.

Sequence similaritiesi

Contains 1 CNH domain.PROSITE-ProRule annotation
Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG238720.
HOGENOMiHOG000141939.
InParanoidiQ06412.
OMAiAAVEIYG.
OrthoDBiEOG7SXWBK.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001180. Citron.
IPR000219. DH-domain.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00036. CNH. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06412-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYRYNRSSPF ERTPEKRVSR QESQRKSIEL PKLPPLNTRN SFLDDSDNGT
60 70 80 90 100
DNISIGWTPI SDTQQFQSPV PQAFTFTSKH SARGNGTSSS ESTPKSTKYV
110 120 130 140 150
KERRPPPPPP LLYSTESIRI DSPMVSPSSQ SRERSPNKLS FIGNSEERHH
160 170 180 190 200
MEYISNHSRI LKSPFANGFS PNSPKSPRDS SKQQAHFSDE SDLRCHEREK
210 220 230 240 250
ALPPIPFTTT LLLSPFDDED SEFFTKPPPP LSTSRNVSGN SRVSEALESV
260 270 280 290 300
YSDSDYTFNN SNARQSSFNS LLGAKPLELA PSITAPTQPF SIQSIDEHKL
310 320 330 340 350
YQCDNVYKLS AIYEWILKVY FEWFNECVFT KIDLFQIVQL LLEFQMPTNF
360 370 380 390 400
DQDTIDSNVD NIMASFISQK AVRFDIINDE EVAVVVGGLD ITGVFTELLP
410 420 430 440 450
CYSFIDNTYG STNSLICYSN VCTHGQSSGF RKEIKLSEII NKSVGLWTEY
460 470 480 490 500
WHLTPDDLAE INPREVQRQS FIFDLIILEE RSLNMATAAV EIYGKRFDKS
510 520 530 540 550
LLPDEPEFKA LAFDIFEPLI QLHTEFLLTP IFWKLKTRGK FIDGVGKIYS
560 570 580 590 600
KWCGEAKNIY LNYAKAMATV HEIIMWEKKN KTKFVTWLKE IDNSVEITRS
610 620 630 640 650
KMYHDVIFFG GFFKSLQNMP VTLRSILKNT DPSMEDYEYL KIVIKEVEKL
660 670 680 690 700
NFEVNQVHGL AIDHRKLVRF SKQLVLSTNS SNATSYVNVG GSTNANDDDA
710 720 730 740 750
IQDKLALGLT YPERKLVLSG TVYKKRDLWL DPTPVYIALL DNCLLITEEI
760 770 780 790 800
SKGETQKYKL IERPIPIDYL SLEKRKIPGT SKQPLRNYSQ KEHKSPMHNF
810 820 830 840 850
STPINSMRPL LKSSGNHMST AYGDRKTSNT EISNANPNTD EFSFKIRNTA
860 870 880 890 900
TGESFKFFTE SAEVLNQWID AIMESFKRNA ENHDLNAFEF TVLSSEFAYF
910 920 930 940 950
DKDAPVNLPV APEGSEIDVA LKAYAQKANK DSCSWSKTTR ILCCEDVKFE
960 970 980 990 1000
GRIYLFVATT DGIYVKYRDD YGSGFVKILE LNDVKRMEAN VKLGLLFVLD
1010 1020 1030 1040 1050
NRKLCYFNIS TVVSRYLAQG NTLDENCIVG TVIRDKVRFF KIADDFGNSK
1060 1070 1080 1090 1100
HLFFERKGKI VILTPEFDQL TNQVKYFKFY KEYKLPSSSN NILNNEIEDI
1110 1120 1130 1140 1150
AIFRKSFAVC TKKTVILYQD SFEDNGIVLP SFLNDKDMMA HLRHPHLNSL
1160 1170 1180 1190 1200
PFKSATDSKK RPSIESLTEE AKKDIATCKA IPVNFFQISQ SSFFALVYDE
1210 1220 1230 1240 1250
AVVKINCYGE MSDWRKDILL LDFCCTGASF HGNHLILVGD NLIQIYDLKN
1260 1270 1280 1290 1300
VEQNLGELVP VQIIKGKKIK LASSERREKT ILVLSHPNIL NRQLLVACNP

VAMADHQ
Length:1,307
Mass (Da):149,113
Last modified:November 1, 1996 - v1
Checksum:i561574AB58FFBC54
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20939 Genomic DNA. Translation: AAB67506.1.
BK006945 Genomic DNA. Translation: DAA09727.1.
PIRiS53412.
RefSeqiNP_013529.3. NM_001182313.3.

Genome annotation databases

EnsemblFungiiYLR425W; YLR425W; YLR425W.
GeneIDi851145.
KEGGisce:YLR425W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20939 Genomic DNA. Translation: AAB67506.1 .
BK006945 Genomic DNA. Translation: DAA09727.1 .
PIRi S53412.
RefSeqi NP_013529.3. NM_001182313.3.

3D structure databases

ProteinModelPortali Q06412.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31685. 72 interactions.
DIPi DIP-4635N.
IntActi Q06412. 4 interactions.
MINTi MINT-486172.
STRINGi 4932.YLR425W.

Proteomic databases

MaxQBi Q06412.
PaxDbi Q06412.
PeptideAtlasi Q06412.
PRIDEi Q06412.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR425W ; YLR425W ; YLR425W .
GeneIDi 851145.
KEGGi sce:YLR425W.

Organism-specific databases

CYGDi YLR425w.
SGDi S000004417. TUS1.

Phylogenomic databases

eggNOGi NOG238720.
HOGENOMi HOG000141939.
InParanoidi Q06412.
OMAi AAVEIYG.
OrthoDBi EOG7SXWBK.

Enzyme and pathway databases

BioCyci YEAST:G3O-32485-MONOMER.

Miscellaneous databases

NextBioi 967912.

Gene expression databases

Genevestigatori Q06412.

Family and domain databases

Gene3Di 1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProi IPR001180. Citron.
IPR000219. DH-domain.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view ]
Pfami PF00780. CNH. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view ]
SMARTi SM00036. CNH. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view ]
SUPFAMi SSF48065. SSF48065. 1 hit.
PROSITEi PS50219. CNH. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast."
    Schmelzle T., Helliwell S.B., Hall M.N.
    Mol. Cell. Biol. 22:1329-1339(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RHO1.
  4. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTUS1_YEAST
AccessioniPrimary (citable) accession number: Q06412
Secondary accession number(s): D6VZ61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3