Rho1 guanine nucleotide exchange factor TUS1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Rho1 guanine nucleotide exchange factor TUS1

Alternative name(s):
TOR unique function suppressor protein 1

Gene names

Name:	TUS1
Synonyms:	SOP10
Ordered Locus Names:	YLR425W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1307 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-exchange factor (GEF) for RHO1 that stimulates the exchange of RHO1 GDP-bound form into GTP-bound form. Required for signaling of cell wall defects to RHO1. Ref.3
Subunit structure	Interacts with RHO1. Ref.3
Domain	The DH domain mediates interaction with RHO1.
Sequence similarities	Contains 1 CNH domain. Contains 1 DH (DBL-homology) domain. Contains 1 PH domain.

Ontologies

Keywords
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fungal-type cell wall organization Inferred from mutant phenotype Ref.3. Source: SGD regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro signal transduction Inferred from mutant phenotype Ref.3. Source: SGD
Cellular_component	cellular bud neck Inferred from direct assay PubMed 16763112. Source: SGD incipient cellular bud site Inferred from direct assay PubMed 18256282. Source: SGD
Molecular_function	Rho guanyl-nucleotide exchange factor activity Inferred from direct assay Ref.3. Source: SGD phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1307

1307

Rho1 guanine nucleotide exchange factor TUS1

PRO_0000080979

Regions

Domain

467 – 657

191

DH

Domain

715 – 877

163

PH

Domain

938 – 1279

342

CNH

Compositional bias

105 – 110

6

Poly-Pro

Compositional bias

227 – 230

4

Poly-Pro

Amino acid modifications

Modified residue

129

1

Phosphoserine Ref.4

Modified residue

170

1

Phosphoserine Ref.5

Modified residue

173

1

Phosphoserine Ref.5

Modified residue

176

1

Phosphoserine Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

Q06412 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 561574AB58FFBC54
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FASTA

1,307

149,113

        10         20         30         40         50         60 
MYRYNRSSPF ERTPEKRVSR QESQRKSIEL PKLPPLNTRN SFLDDSDNGT DNISIGWTPI 

        70         80         90        100        110        120 
SDTQQFQSPV PQAFTFTSKH SARGNGTSSS ESTPKSTKYV KERRPPPPPP LLYSTESIRI 

       130        140        150        160        170        180 
DSPMVSPSSQ SRERSPNKLS FIGNSEERHH MEYISNHSRI LKSPFANGFS PNSPKSPRDS 

       190        200        210        220        230        240 
SKQQAHFSDE SDLRCHEREK ALPPIPFTTT LLLSPFDDED SEFFTKPPPP LSTSRNVSGN 

       250        260        270        280        290        300 
SRVSEALESV YSDSDYTFNN SNARQSSFNS LLGAKPLELA PSITAPTQPF SIQSIDEHKL 

       310        320        330        340        350        360 
YQCDNVYKLS AIYEWILKVY FEWFNECVFT KIDLFQIVQL LLEFQMPTNF DQDTIDSNVD 

       370        380        390        400        410        420 
NIMASFISQK AVRFDIINDE EVAVVVGGLD ITGVFTELLP CYSFIDNTYG STNSLICYSN 

       430        440        450        460        470        480 
VCTHGQSSGF RKEIKLSEII NKSVGLWTEY WHLTPDDLAE INPREVQRQS FIFDLIILEE 

       490        500        510        520        530        540 
RSLNMATAAV EIYGKRFDKS LLPDEPEFKA LAFDIFEPLI QLHTEFLLTP IFWKLKTRGK 

       550        560        570        580        590        600 
FIDGVGKIYS KWCGEAKNIY LNYAKAMATV HEIIMWEKKN KTKFVTWLKE IDNSVEITRS 

       610        620        630        640        650        660 
KMYHDVIFFG GFFKSLQNMP VTLRSILKNT DPSMEDYEYL KIVIKEVEKL NFEVNQVHGL 

       670        680        690        700        710        720 
AIDHRKLVRF SKQLVLSTNS SNATSYVNVG GSTNANDDDA IQDKLALGLT YPERKLVLSG 

       730        740        750        760        770        780 
TVYKKRDLWL DPTPVYIALL DNCLLITEEI SKGETQKYKL IERPIPIDYL SLEKRKIPGT 

       790        800        810        820        830        840 
SKQPLRNYSQ KEHKSPMHNF STPINSMRPL LKSSGNHMST AYGDRKTSNT EISNANPNTD 

       850        860        870        880        890        900 
EFSFKIRNTA TGESFKFFTE SAEVLNQWID AIMESFKRNA ENHDLNAFEF TVLSSEFAYF 

       910        920        930        940        950        960 
DKDAPVNLPV APEGSEIDVA LKAYAQKANK DSCSWSKTTR ILCCEDVKFE GRIYLFVATT 

       970        980        990       1000       1010       1020 
DGIYVKYRDD YGSGFVKILE LNDVKRMEAN VKLGLLFVLD NRKLCYFNIS TVVSRYLAQG 

      1030       1040       1050       1060       1070       1080 
NTLDENCIVG TVIRDKVRFF KIADDFGNSK HLFFERKGKI VILTPEFDQL TNQVKYFKFY 

      1090       1100       1110       1120       1130       1140 
KEYKLPSSSN NILNNEIEDI AIFRKSFAVC TKKTVILYQD SFEDNGIVLP SFLNDKDMMA 

      1150       1160       1170       1180       1190       1200 
HLRHPHLNSL PFKSATDSKK RPSIESLTEE AKKDIATCKA IPVNFFQISQ SSFFALVYDE 

      1210       1220       1230       1240       1250       1260 
AVVKINCYGE MSDWRKDILL LDFCCTGASF HGNHLILVGD NLIQIYDLKN VEQNLGELVP 

      1270       1280       1290       1300 
VQIIKGKKIK LASSERREKT ILVLSHPNIL NRQLLVACNP VAMADHQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast." Schmelzle T., Helliwell S.B., Hall M.N. Mol. Cell. Biol. 22:1329-1339(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RHO1.
[4]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129, MASS SPECTROMETRY.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-170; SER-173 AND SER-176, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20939 Genomic DNA. Translation: AAB67506.1. BK006945 Genomic DNA. Translation: DAA09727.1.
PIR	S53412.
RefSeq	NP_013529.3. NM_001182313.3.
3D structure databases
ProteinModelPortal	Q06412.
SMR	Q06412. Positions 465-656.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4635N.
IntAct	Q06412. 4 interactions.
MINT	MINT-486172.
STRING	4932.YLR425W.
Proteomic databases
PaxDb	Q06412.
PeptideAtlas	Q06412.
PRIDE	Q06412.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR425W; YLR425W; YLR425W.
GeneID	851145.
KEGG	sce:YLR425W. sce:YLR427W.
Organism-specific databases
CYGD	YLR425w.
SGD	S000004417. TUS1.
Phylogenomic databases
eggNOG	NOG238720.
HOGENOM	HOG000141939.
OMA	AAVEIYG.
OrthoDB	EOG4WHCTT.
Gene expression databases
Genevestigator	Q06412.
GermOnline	YLR425W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.20.900.10. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR001180. Citron. IPR000219. DH-domain. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. [Graphical view]
Pfam	PF00780. CNH. 1 hit. PF00169. PH. 1 hit. PF00621. RhoGEF. 1 hit. [Graphical view]
SMART	SM00036. CNH. 1 hit. SM00233. PH. 1 hit. SM00325. RhoGEF. 1 hit. [Graphical view]
SUPFAM	SSF48065. DH-domain. 1 hit.
PROSITE	PS50219. CNH. 1 hit. PS00741. DH_1. False negative. PS50010. DH_2. 1 hit. PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967912.

Entry information

Entry name

TUS1_YEAST

Accession

Primary (citable) accession number: Q06412
Secondary accession number(s): D6VZ61

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents