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Q06412

- TUS1_YEAST

UniProt

Q06412 - TUS1_YEAST

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Protein
Rho1 guanine nucleotide exchange factor TUS1
Gene
TUS1, SOP10, YLR425W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Guanine nucleotide-exchange factor (GEF) for RHO1 that stimulates the exchange of RHO1 GDP-bound form into GTP-bound form. Required for signaling of cell wall defects to RHO1.1 Publication

GO - Molecular functioni

  1. Rho guanyl-nucleotide exchange factor activity Source: SGD
  2. protein binding Source: IntAct
  3. small GTPase regulator activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. fungal-type cell wall organization Source: SGD
  2. regulation of Rho GTPase activity Source: GOC
  3. signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciYEAST:G3O-32485-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho1 guanine nucleotide exchange factor TUS1
Alternative name(s):
TOR unique function suppressor protein 1
Gene namesi
Name:TUS1
Synonyms:SOP10
Ordered Locus Names:YLR425W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR425w.
SGDiS000004417. TUS1.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. incipient cellular bud site Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13071307Rho1 guanine nucleotide exchange factor TUS1
PRO_0000080979Add
BLAST

Proteomic databases

MaxQBiQ06412.
PaxDbiQ06412.
PeptideAtlasiQ06412.
PRIDEiQ06412.

Expressioni

Gene expression databases

GenevestigatoriQ06412.

Interactioni

Subunit structurei

Interacts with RHO1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SHO1P400733EBI-37117,EBI-18140

Protein-protein interaction databases

BioGridi31685. 71 interactions.
DIPiDIP-4635N.
IntActiQ06412. 4 interactions.
MINTiMINT-486172.
STRINGi4932.YLR425W.

Structurei

3D structure databases

ProteinModelPortaliQ06412.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini467 – 657191DH
Add
BLAST
Domaini715 – 877163PH
Add
BLAST
Domaini938 – 1279342CNH
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi105 – 1106Poly-Pro
Compositional biasi227 – 2304Poly-Pro

Domaini

The DH domain mediates interaction with RHO1.

Sequence similaritiesi

Contains 1 CNH domain.
Contains 1 PH domain.

Phylogenomic databases

eggNOGiNOG238720.
HOGENOMiHOG000141939.
OMAiAAVEIYG.
OrthoDBiEOG7SXWBK.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001180. Citron.
IPR000219. DH-domain.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00036. CNH. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06412-1 [UniParc]FASTAAdd to Basket

« Hide

MYRYNRSSPF ERTPEKRVSR QESQRKSIEL PKLPPLNTRN SFLDDSDNGT     50
DNISIGWTPI SDTQQFQSPV PQAFTFTSKH SARGNGTSSS ESTPKSTKYV 100
KERRPPPPPP LLYSTESIRI DSPMVSPSSQ SRERSPNKLS FIGNSEERHH 150
MEYISNHSRI LKSPFANGFS PNSPKSPRDS SKQQAHFSDE SDLRCHEREK 200
ALPPIPFTTT LLLSPFDDED SEFFTKPPPP LSTSRNVSGN SRVSEALESV 250
YSDSDYTFNN SNARQSSFNS LLGAKPLELA PSITAPTQPF SIQSIDEHKL 300
YQCDNVYKLS AIYEWILKVY FEWFNECVFT KIDLFQIVQL LLEFQMPTNF 350
DQDTIDSNVD NIMASFISQK AVRFDIINDE EVAVVVGGLD ITGVFTELLP 400
CYSFIDNTYG STNSLICYSN VCTHGQSSGF RKEIKLSEII NKSVGLWTEY 450
WHLTPDDLAE INPREVQRQS FIFDLIILEE RSLNMATAAV EIYGKRFDKS 500
LLPDEPEFKA LAFDIFEPLI QLHTEFLLTP IFWKLKTRGK FIDGVGKIYS 550
KWCGEAKNIY LNYAKAMATV HEIIMWEKKN KTKFVTWLKE IDNSVEITRS 600
KMYHDVIFFG GFFKSLQNMP VTLRSILKNT DPSMEDYEYL KIVIKEVEKL 650
NFEVNQVHGL AIDHRKLVRF SKQLVLSTNS SNATSYVNVG GSTNANDDDA 700
IQDKLALGLT YPERKLVLSG TVYKKRDLWL DPTPVYIALL DNCLLITEEI 750
SKGETQKYKL IERPIPIDYL SLEKRKIPGT SKQPLRNYSQ KEHKSPMHNF 800
STPINSMRPL LKSSGNHMST AYGDRKTSNT EISNANPNTD EFSFKIRNTA 850
TGESFKFFTE SAEVLNQWID AIMESFKRNA ENHDLNAFEF TVLSSEFAYF 900
DKDAPVNLPV APEGSEIDVA LKAYAQKANK DSCSWSKTTR ILCCEDVKFE 950
GRIYLFVATT DGIYVKYRDD YGSGFVKILE LNDVKRMEAN VKLGLLFVLD 1000
NRKLCYFNIS TVVSRYLAQG NTLDENCIVG TVIRDKVRFF KIADDFGNSK 1050
HLFFERKGKI VILTPEFDQL TNQVKYFKFY KEYKLPSSSN NILNNEIEDI 1100
AIFRKSFAVC TKKTVILYQD SFEDNGIVLP SFLNDKDMMA HLRHPHLNSL 1150
PFKSATDSKK RPSIESLTEE AKKDIATCKA IPVNFFQISQ SSFFALVYDE 1200
AVVKINCYGE MSDWRKDILL LDFCCTGASF HGNHLILVGD NLIQIYDLKN 1250
VEQNLGELVP VQIIKGKKIK LASSERREKT ILVLSHPNIL NRQLLVACNP 1300
VAMADHQ 1307
Length:1,307
Mass (Da):149,113
Last modified:November 1, 1996 - v1
Checksum:i561574AB58FFBC54
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20939 Genomic DNA. Translation: AAB67506.1.
BK006945 Genomic DNA. Translation: DAA09727.1.
PIRiS53412.
RefSeqiNP_013529.3. NM_001182313.3.

Genome annotation databases

EnsemblFungiiYLR425W; YLR425W; YLR425W.
GeneIDi851145.
KEGGisce:YLR425W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20939 Genomic DNA. Translation: AAB67506.1 .
BK006945 Genomic DNA. Translation: DAA09727.1 .
PIRi S53412.
RefSeqi NP_013529.3. NM_001182313.3.

3D structure databases

ProteinModelPortali Q06412.
ModBasei Search...

Protein-protein interaction databases

BioGridi 31685. 71 interactions.
DIPi DIP-4635N.
IntActi Q06412. 4 interactions.
MINTi MINT-486172.
STRINGi 4932.YLR425W.

Proteomic databases

MaxQBi Q06412.
PaxDbi Q06412.
PeptideAtlasi Q06412.
PRIDEi Q06412.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR425W ; YLR425W ; YLR425W .
GeneIDi 851145.
KEGGi sce:YLR425W.

Organism-specific databases

CYGDi YLR425w.
SGDi S000004417. TUS1.

Phylogenomic databases

eggNOGi NOG238720.
HOGENOMi HOG000141939.
OMAi AAVEIYG.
OrthoDBi EOG7SXWBK.

Enzyme and pathway databases

BioCyci YEAST:G3O-32485-MONOMER.

Miscellaneous databases

NextBioi 967912.

Gene expression databases

Genevestigatori Q06412.

Family and domain databases

Gene3Di 1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProi IPR001180. Citron.
IPR000219. DH-domain.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view ]
Pfami PF00780. CNH. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view ]
SMARTi SM00036. CNH. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view ]
SUPFAMi SSF48065. SSF48065. 1 hit.
PROSITEi PS50219. CNH. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast."
    Schmelzle T., Helliwell S.B., Hall M.N.
    Mol. Cell. Biol. 22:1329-1339(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RHO1.
  4. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTUS1_YEAST
AccessioniPrimary (citable) accession number: Q06412
Secondary accession number(s): D6VZ61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

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