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Q06412

- TUS1_YEAST

UniProt

Q06412 - TUS1_YEAST

Protein

Rho1 guanine nucleotide exchange factor TUS1

Gene

TUS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-exchange factor (GEF) for RHO1 that stimulates the exchange of RHO1 GDP-bound form into GTP-bound form. Required for signaling of cell wall defects to RHO1.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. Rho guanyl-nucleotide exchange factor activity Source: SGD
    3. small GTPase regulator activity Source: InterPro

    GO - Biological processi

    1. fungal-type cell wall organization Source: SGD
    2. positive regulation of Rho GTPase activity Source: GOC
    3. signal transduction Source: SGD

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32485-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho1 guanine nucleotide exchange factor TUS1
    Alternative name(s):
    TOR unique function suppressor protein 1
    Gene namesi
    Name:TUS1
    Synonyms:SOP10
    Ordered Locus Names:YLR425W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR425w.
    SGDiS000004417. TUS1.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. incipient cellular bud site Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13071307Rho1 guanine nucleotide exchange factor TUS1PRO_0000080979Add
    BLAST

    Proteomic databases

    MaxQBiQ06412.
    PaxDbiQ06412.
    PeptideAtlasiQ06412.
    PRIDEiQ06412.

    Expressioni

    Gene expression databases

    GenevestigatoriQ06412.

    Interactioni

    Subunit structurei

    Interacts with RHO1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SHO1P400733EBI-37117,EBI-18140

    Protein-protein interaction databases

    BioGridi31685. 71 interactions.
    DIPiDIP-4635N.
    IntActiQ06412. 4 interactions.
    MINTiMINT-486172.
    STRINGi4932.YLR425W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06412.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini467 – 657191DHPROSITE-ProRule annotationAdd
    BLAST
    Domaini715 – 877163PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini938 – 1279342CNHPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi105 – 1106Poly-Pro
    Compositional biasi227 – 2304Poly-Pro

    Domaini

    The DH domain mediates interaction with RHO1.

    Sequence similaritiesi

    Contains 1 CNH domain.PROSITE-ProRule annotation
    Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG238720.
    HOGENOMiHOG000141939.
    OMAiAAVEIYG.
    OrthoDBiEOG7SXWBK.

    Family and domain databases

    Gene3Di1.20.900.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR001180. Citron.
    IPR000219. DH-domain.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view]
    PfamiPF00780. CNH. 1 hit.
    PF00169. PH. 1 hit.
    PF00621. RhoGEF. 1 hit.
    [Graphical view]
    SMARTiSM00036. CNH. 1 hit.
    SM00233. PH. 1 hit.
    SM00325. RhoGEF. 1 hit.
    [Graphical view]
    SUPFAMiSSF48065. SSF48065. 1 hit.
    PROSITEiPS50219. CNH. 1 hit.
    PS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q06412-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYRYNRSSPF ERTPEKRVSR QESQRKSIEL PKLPPLNTRN SFLDDSDNGT     50
    DNISIGWTPI SDTQQFQSPV PQAFTFTSKH SARGNGTSSS ESTPKSTKYV 100
    KERRPPPPPP LLYSTESIRI DSPMVSPSSQ SRERSPNKLS FIGNSEERHH 150
    MEYISNHSRI LKSPFANGFS PNSPKSPRDS SKQQAHFSDE SDLRCHEREK 200
    ALPPIPFTTT LLLSPFDDED SEFFTKPPPP LSTSRNVSGN SRVSEALESV 250
    YSDSDYTFNN SNARQSSFNS LLGAKPLELA PSITAPTQPF SIQSIDEHKL 300
    YQCDNVYKLS AIYEWILKVY FEWFNECVFT KIDLFQIVQL LLEFQMPTNF 350
    DQDTIDSNVD NIMASFISQK AVRFDIINDE EVAVVVGGLD ITGVFTELLP 400
    CYSFIDNTYG STNSLICYSN VCTHGQSSGF RKEIKLSEII NKSVGLWTEY 450
    WHLTPDDLAE INPREVQRQS FIFDLIILEE RSLNMATAAV EIYGKRFDKS 500
    LLPDEPEFKA LAFDIFEPLI QLHTEFLLTP IFWKLKTRGK FIDGVGKIYS 550
    KWCGEAKNIY LNYAKAMATV HEIIMWEKKN KTKFVTWLKE IDNSVEITRS 600
    KMYHDVIFFG GFFKSLQNMP VTLRSILKNT DPSMEDYEYL KIVIKEVEKL 650
    NFEVNQVHGL AIDHRKLVRF SKQLVLSTNS SNATSYVNVG GSTNANDDDA 700
    IQDKLALGLT YPERKLVLSG TVYKKRDLWL DPTPVYIALL DNCLLITEEI 750
    SKGETQKYKL IERPIPIDYL SLEKRKIPGT SKQPLRNYSQ KEHKSPMHNF 800
    STPINSMRPL LKSSGNHMST AYGDRKTSNT EISNANPNTD EFSFKIRNTA 850
    TGESFKFFTE SAEVLNQWID AIMESFKRNA ENHDLNAFEF TVLSSEFAYF 900
    DKDAPVNLPV APEGSEIDVA LKAYAQKANK DSCSWSKTTR ILCCEDVKFE 950
    GRIYLFVATT DGIYVKYRDD YGSGFVKILE LNDVKRMEAN VKLGLLFVLD 1000
    NRKLCYFNIS TVVSRYLAQG NTLDENCIVG TVIRDKVRFF KIADDFGNSK 1050
    HLFFERKGKI VILTPEFDQL TNQVKYFKFY KEYKLPSSSN NILNNEIEDI 1100
    AIFRKSFAVC TKKTVILYQD SFEDNGIVLP SFLNDKDMMA HLRHPHLNSL 1150
    PFKSATDSKK RPSIESLTEE AKKDIATCKA IPVNFFQISQ SSFFALVYDE 1200
    AVVKINCYGE MSDWRKDILL LDFCCTGASF HGNHLILVGD NLIQIYDLKN 1250
    VEQNLGELVP VQIIKGKKIK LASSERREKT ILVLSHPNIL NRQLLVACNP 1300
    VAMADHQ 1307
    Length:1,307
    Mass (Da):149,113
    Last modified:November 1, 1996 - v1
    Checksum:i561574AB58FFBC54
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20939 Genomic DNA. Translation: AAB67506.1.
    BK006945 Genomic DNA. Translation: DAA09727.1.
    PIRiS53412.
    RefSeqiNP_013529.3. NM_001182313.3.

    Genome annotation databases

    EnsemblFungiiYLR425W; YLR425W; YLR425W.
    GeneIDi851145.
    KEGGisce:YLR425W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20939 Genomic DNA. Translation: AAB67506.1 .
    BK006945 Genomic DNA. Translation: DAA09727.1 .
    PIRi S53412.
    RefSeqi NP_013529.3. NM_001182313.3.

    3D structure databases

    ProteinModelPortali Q06412.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31685. 71 interactions.
    DIPi DIP-4635N.
    IntActi Q06412. 4 interactions.
    MINTi MINT-486172.
    STRINGi 4932.YLR425W.

    Proteomic databases

    MaxQBi Q06412.
    PaxDbi Q06412.
    PeptideAtlasi Q06412.
    PRIDEi Q06412.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR425W ; YLR425W ; YLR425W .
    GeneIDi 851145.
    KEGGi sce:YLR425W.

    Organism-specific databases

    CYGDi YLR425w.
    SGDi S000004417. TUS1.

    Phylogenomic databases

    eggNOGi NOG238720.
    HOGENOMi HOG000141939.
    OMAi AAVEIYG.
    OrthoDBi EOG7SXWBK.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32485-MONOMER.

    Miscellaneous databases

    NextBioi 967912.

    Gene expression databases

    Genevestigatori Q06412.

    Family and domain databases

    Gene3Di 1.20.900.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR001180. Citron.
    IPR000219. DH-domain.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view ]
    Pfami PF00780. CNH. 1 hit.
    PF00169. PH. 1 hit.
    PF00621. RhoGEF. 1 hit.
    [Graphical view ]
    SMARTi SM00036. CNH. 1 hit.
    SM00233. PH. 1 hit.
    SM00325. RhoGEF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48065. SSF48065. 1 hit.
    PROSITEi PS50219. CNH. 1 hit.
    PS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast."
      Schmelzle T., Helliwell S.B., Hall M.N.
      Mol. Cell. Biol. 22:1329-1339(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RHO1.
    4. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTUS1_YEAST
    AccessioniPrimary (citable) accession number: Q06412
    Secondary accession number(s): D6VZ61
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3