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Protein

Pre-mRNA-splicing factor SPP382

Gene

SPP382

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing and spliceosome disassembly. Promotes release of excised lariat intron from the spliceosome by acting as a receptor for PRP43. This targeting of PRP43 leads to disassembly of the spliceosome with the separation of the U2, U5, U6 snRNPs and the NTC complex.2 Publications

GO - Molecular functioni

GO - Biological processi

  • generation of catalytic spliceosome for first transesterification step Source: SGD
  • negative regulation of DNA ligase activity Source: BHF-UCL
  • negative regulation of double-strand break repair via nonhomologous end joining Source: BHF-UCL
  • negative regulation of protein binding Source: BHF-UCL
  • negative regulation of protein complex assembly Source: BHF-UCL
  • spliceosomal complex disassembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-32484-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor SPP382
Alternative name(s):
CLF1 complex factor 8
Nineteen complex-related protein 1
Short name:
NTC-related protein 1
Suppressor of PRP38 protein 2
Gene namesi
Name:SPP382
Synonyms:CCF8, NTR1
Ordered Locus Names:YLR424W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR424W.
SGDiS000004416. SPP382.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nuclear chromosome, telomeric region Source: BHF-UCL
  • nucleolus Source: BHF-UCL
  • spliceosomal complex Source: SGD
  • U2-type post-mRNA release spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 708708Pre-mRNA-splicing factor SPP382PRO_0000270560Add
BLAST

Proteomic databases

MaxQBiQ06411.

Interactioni

Subunit structurei

Component of the NTR complex (NTC-related complex), composed of NTR1, NTR2 and PRP43. Interacts with CLF1, NTR2 and PRP43.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CEF1Q036543EBI-576,EBI-476
NTR2P3611811EBI-576,EBI-26362
PRP43P5313112EBI-576,EBI-505

Protein-protein interaction databases

BioGridi31683. 82 interactions.
DIPiDIP-1491N.
IntActiQ06411. 128 interactions.
MINTiMINT-403663.

Structurei

3D structure databases

ProteinModelPortaliQ06411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 10848G-patchPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 G-patch domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000067729.
InParanoidiQ06411.
KOiK13103.
OMAiNKILYQW.
OrthoDBiEOG092C1UTF.

Family and domain databases

InterProiIPR000467. G_patch_dom.
[Graphical view]
PfamiPF01585. G-patch. 1 hit.
[Graphical view]
SMARTiSM00443. G_patch. 1 hit.
[Graphical view]
PROSITEiPS50174. G_PATCH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDSDSNTDK KFFFKKRRID SYNYSDEEDN NSSMNSDMTY TNDALKTSSG
60 70 80 90 100
NAPTISKLTK TYGIGAKLLS SMGYVAGKGL GKDGSGITTP IETQSRPMHN
110 120 130 140 150
AGLGMFSNTN SSNYHSENED YLSSEDEVVE GIEQVKFNKT STEVLGEALL
160 170 180 190 200
NDSGDMTIVR TLRELRLAGV QLPESILKEL DPLNAVPKPK KDVVVEILQE
210 220 230 240 250
LLGIEKSLEA IRQRTSPLEV QVKEYYGQER LLSELEVTLR DESKHVSLYD
260 270 280 290 300
KIGAILKLSD DELIDRLTSC LLRKELLIEF DLDHLEKPND ILDELTQIIE
310 320 330 340 350
LLAYRMDTTS KFLNRTQTTI FKVIYPKLKK FWEGFDMTKS KIDSAITLLL
360 370 380 390 400
DFQQVLSFIG CKEHIMEEFV YPKLLQELDN WELHDEVDHV SPRIWVLDFM
410 420 430 440 450
VLIDDKIKDT IVDKIEAKFF AYCKNWYHRE SFCITNSDII FIKELICERR
460 470 480 490 500
YYKILCKEFL PKFLDELWER HNDPIYELED WKEKQEWKEK DSGFFYFMKK
510 520 530 540 550
LRSYTHYFHP KQYELMMRGT FNNINKILYQ WHLYSTVEDL HKSKWWLNWL
560 570 580 590 600
MNTVFEHSLP TEIELSEIRK SYNIFAMSHR YHLDKSTLDE DFDLRQGLRN
610 620 630 640 650
LMETQVIDDI SQSEQEPTYT VQNIPLGKVS SSFKDVVEDY CLEKGYLISK
660 670 680 690 700
IPNRYTQLPY GRDQDCIVPL FEIRNGKKKM EVALKHDILW VEDSSGTFKP

IYLWALDL
Length:708
Mass (Da):83,049
Last modified:November 1, 1996 - v1
Checksum:i9986B547B9582338
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20939 Genomic DNA. Translation: AAB67507.1.
BK006945 Genomic DNA. Translation: DAA09726.1.
PIRiS53411.
RefSeqiNP_013528.1. NM_001182312.1.

Genome annotation databases

EnsemblFungiiYLR424W; YLR424W; YLR424W.
GeneIDi851143.
KEGGisce:YLR424W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20939 Genomic DNA. Translation: AAB67507.1.
BK006945 Genomic DNA. Translation: DAA09726.1.
PIRiS53411.
RefSeqiNP_013528.1. NM_001182312.1.

3D structure databases

ProteinModelPortaliQ06411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31683. 82 interactions.
DIPiDIP-1491N.
IntActiQ06411. 128 interactions.
MINTiMINT-403663.

Proteomic databases

MaxQBiQ06411.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR424W; YLR424W; YLR424W.
GeneIDi851143.
KEGGisce:YLR424W.

Organism-specific databases

EuPathDBiFungiDB:YLR424W.
SGDiS000004416. SPP382.

Phylogenomic databases

HOGENOMiHOG000067729.
InParanoidiQ06411.
KOiK13103.
OMAiNKILYQW.
OrthoDBiEOG092C1UTF.

Enzyme and pathway databases

BioCyciYEAST:G3O-32484-MONOMER.

Miscellaneous databases

PROiQ06411.

Family and domain databases

InterProiIPR000467. G_patch_dom.
[Graphical view]
PfamiPF01585. G-patch. 1 hit.
[Graphical view]
SMARTiSM00443. G_patch. 1 hit.
[Graphical view]
PROSITEiPS50174. G_PATCH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSP382_YEAST
AccessioniPrimary (citable) accession number: Q06411
Secondary accession number(s): D6VZ60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 556 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.