Q06411 (SP382_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor SPP382

Alternative name(s):
CLF1 complex factor 8
Nineteen complex-related protein 1
Short name=NTC-related protein 1
Suppressor of PRP38 protein 2

Gene names

Name:	SPP382
Synonyms:	CCF8, NTR1
Ordered Locus Names:	YLR424W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	708 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing and spliceosome disassembly. Promotes release of excised lariat intron from the spliceosome by acting as a receptor for PRP43. This targeting of PRP43 leads to disassembly of the spliceosome with the separation of the U2, U5, U6 snRNPs and the NTC complex. Ref.6 Ref.7
Subunit structure	Component of the NTR complex (NTC-related complex), composed of NTR1, NTR2 and PRP43. Interacts with CLF1, NTR2 and PRP43. Ref.3 Ref.6 Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.4.
Miscellaneous	Present with 556 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 1 G-patch domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus Spliceosome
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	generation of catalytic spliceosome for first transesterification step Inferred from mutant phenotype. Source: SGD spliceosome disassembly Inferred from direct assay Ref.6Ref.7. Source: SGD
Cellular component	U2-type post-mRNA release spliceosomal complex Inferred from direct assay Ref.6. Source: SGD mitochondrion Inferred from direct assay. Source: SGD
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.6. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CEF1	Q03654	3	EBI-576,EBI-476
NTR2	P36118	11	EBI-576,EBI-26362
PRP43	P53131	10	EBI-576,EBI-505

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 708	708	Pre-mRNA-splicing factor SPP382		PRO_0000270560
Regions
	Domain	61 – 108	48	G-patch

Sequences

	Sequence		Length	Mass (Da)
	Q06411 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9986B547B9582338	FASTA	708	83,049
	10 20 30 40 50 60 MEDSDSNTDK KFFFKKRRID SYNYSDEEDN NSSMNSDMTY TNDALKTSSG NAPTISKLTK 70 80 90 100 110 120 TYGIGAKLLS SMGYVAGKGL GKDGSGITTP IETQSRPMHN AGLGMFSNTN SSNYHSENED 130 140 150 160 170 180 YLSSEDEVVE GIEQVKFNKT STEVLGEALL NDSGDMTIVR TLRELRLAGV QLPESILKEL 190 200 210 220 230 240 DPLNAVPKPK KDVVVEILQE LLGIEKSLEA IRQRTSPLEV QVKEYYGQER LLSELEVTLR 250 260 270 280 290 300 DESKHVSLYD KIGAILKLSD DELIDRLTSC LLRKELLIEF DLDHLEKPND ILDELTQIIE 310 320 330 340 350 360 LLAYRMDTTS KFLNRTQTTI FKVIYPKLKK FWEGFDMTKS KIDSAITLLL DFQQVLSFIG 370 380 390 400 410 420 CKEHIMEEFV YPKLLQELDN WELHDEVDHV SPRIWVLDFM VLIDDKIKDT IVDKIEAKFF 430 440 450 460 470 480 AYCKNWYHRE SFCITNSDII FIKELICERR YYKILCKEFL PKFLDELWER HNDPIYELED 490 500 510 520 530 540 WKEKQEWKEK DSGFFYFMKK LRSYTHYFHP KQYELMMRGT FNNINKILYQ WHLYSTVEDL 550 560 570 580 590 600 HKSKWWLNWL MNTVFEHSLP TEIELSEIRK SYNIFAMSHR YHLDKSTLDE DFDLRQGLRN 610 620 630 640 650 660 LMETQVIDDI SQSEQEPTYT VQNIPLGKVS SSFKDVVEDY CLEKGYLISK IPNRYTQLPY 670 680 690 700 GRDQDCIVPL FEIRNGKKKM EVALKHDILW VEDSSGTFKP IYLWALDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The Clf1p splicing factor promotes spliceosome assembly through N-terminal tetratricopeptide repeat contacts." Wang Q., Hobbs K., Lynn B., Rymond B.C. J. Biol. Chem. 278:7875-7883(2003) [PubMed: 12509417] [Abstract] Cited for: INTERACTION WITH CLF1.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2." Tsai R.-T., Fu R.-H., Yeh F.-L., Tseng C.-K., Lin Y.-C., Huang Y.-H., Cheng S.-C. Genes Dev. 19:2991-3003(2005) [PubMed: 16357217] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE NTC-RELATED COMPLEX, INTERACTION WITH CLF1; NTR2 AND PRP43.
[7]	"Yeast ntr1/spp382 mediates prp43 function in postspliceosomes." Boon K.-L., Auchynnikava T., Edwalds-Gilbert G., Barrass J.D., Droop A.P., Dez C., Beggs J.D. Mol. Cell. Biol. 26:6016-6023(2006) [PubMed: 16880513] [Abstract] Cited for: FUNCTION, INTERACTION WITH NTR2 AND PRP43.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20939 Genomic DNA. Translation: AAB67507.1. BK006945 Genomic DNA. Translation: DAA09726.1.
PIR	S53411.
RefSeq	NP_013528.1. NM_001182312.1.
3D structure databases
ProteinModelPortal	Q06411.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1491N.
IntAct	Q06411. 130 interactions.
MINT	MINT-403663.
STRING	Q06411.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR424W; YLR424W; YLR424W.
GeneID	851143.
KEGG	sce:YLR424W.
NMPDR	fig|4932.3.peg.4558.
Organism-specific databases
CYGD	YLR424w.
SGD	S000004416. SPP382.
Phylogenomic databases
eggNOG	fuNOG07570.
GeneTree	EFGT00050000005891.
OMA	LYQWHLY.
OrthoDB	EOG4K0TXN.
Gene expression databases
ArrayExpress	Q06411.
Genevestigator	Q06411.
Family and domain databases
InterPro	IPR000467. G_patch. [Graphical view]
KO	K13103.
Pfam	PF01585. G-patch. 1 hit. [Graphical view]
SMART	SM00443. G_patch. 1 hit. [Graphical view]
PROSITE	PS50174. G_PATCH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967907.

Entry information

Entry name

SP382_YEAST

Accession

Primary (citable) accession number: Q06411
Secondary accession number(s): D6VZ60

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program