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Protein

Transaminated amino acid decarboxylase

Gene

ARO10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One of five 2-oxo acid decarboxylases (PDC1, PDC5, PDC6, ARO10, and THI3) involved in amino acid catabolism. The enzyme catalyzes the decarboxylation of amino acids, which, in a first step, have been transaminated to the corresponding 2-oxo acids (alpha-keto-acids). In a third step, the resulting aldehydes are reduced to alcohols, collectively referred to as fusel oils or alcohols. Its preferred substrates are the transaminated amino acids, phenylalanine, tryptophan, (and probably tyrosine), but also isoleucine, whereas leucine is a low efficiency and valine and pyruvate are no substrates. In analogy to the pyruvate decarboxylases the enzyme may in a side-reaction catalyze condensation (or carboligation) reactions leading to the formation of 2-hydroxy ketone, collectively called acyloins.3 Publications

Catalytic activityi

A 2-oxo acid = an aldehyde + CO2.
Phenylpyruvate = phenylacetaldehyde + CO2.
3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ per subunit.By similarity
  • thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity

Pathwayi: Ehrlich pathway

This protein is involved in the pathway Ehrlich pathway, which is part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the pathway Ehrlich pathway and in Amino-acid degradation.

GO - Molecular functioni

  • carboxy-lyase activity Source: SGD
  • magnesium ion binding Source: InterPro
  • phenylpyruvate decarboxylase activity Source: SGD
  • thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  • aromatic amino acid family catabolic process to alcohol via Ehrlich pathway Source: SGD
  • branched-chain amino acid catabolic process to alcohol via Ehrlich pathway Source: SGD
  • leucine catabolic process Source: SGD
  • L-phenylalanine catabolic process Source: SGD
  • methionine catabolic process to 3-methylthiopropanol Source: SGD
  • tryptophan catabolic process Source: SGD
  • tyrosine catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Branched-chain amino acid catabolism, Phenylalanine catabolism, Tryptophan catabolism, Tyrosine catabolism

Keywords - Ligandi

Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11837.
YEAST:MONOMER3O-398.
BRENDAi4.1.1.43. 984.
UniPathwayiUPA00866.

Names & Taxonomyi

Protein namesi
Recommended name:
Transaminated amino acid decarboxylase (EC:4.1.1.43)
Alternative name(s):
Transaminated branched-chain amino acid decarboxylase
Gene namesi
Name:ARO10
Ordered Locus Names:YDR380W
ORF Names:D9481.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR380W.
SGDiS000002788. ARO10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Biotechnological usei

Fusel oils are important flavor and aroma compounds in yeast-fermented products contributing to the quality of beverages and food. In low concentration they are generally desirable, whereas high concentrations may spoil the product. By adjusting growth conditions and substrate their production is sought to be influenced. Phenylethanol, having a rose-like aroma, is an important fragrance in the cosmetic industry and can be produced by fermentation.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 635635Transaminated amino acid decarboxylasePRO_0000090835Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki588 – 588Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ06408.

Expressioni

Inductioni

Expression is induced by the presence of aromatic amino acids and the lack of preferred nitrogen sources (e.g. ammonia, glutamine) and requires the transcription activator ARO80.

Interactioni

Protein-protein interaction databases

BioGridi32440. 14 interactions.
DIPiDIP-5252N.
IntActiQ06408. 2 interactions.
MINTiMINT-546662.

Structurei

3D structure databases

ProteinModelPortaliQ06408.
SMRiQ06408. Positions 23-630.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075465.
HOGENOMiHOG000061334.
InParanoidiQ06408.
KOiK12732.
OMAiRSYNDIQ.
OrthoDBiEOG092C2L8F.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR012110. TPP_enzyme.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF036565. Pyruvt_ip_decrb. 1 hit.
SUPFAMiSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.

Sequencei

Sequence statusi: Complete.

Q06408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPVTIEKFV NQEERHLVSN RSATIPFGEY IFKRLLSIDT KSVFGVPGDF
60 70 80 90 100
NLSLLEYLYS PSVESAGLRW VGTCNELNAA YAADGYSRYS NKIGCLITTY
110 120 130 140 150
GVGELSALNG IAGSFAENVK VLHIVGVAKS IDSRSSNFSD RNLHHLVPQL
160 170 180 190 200
HDSNFKGPNH KVYHDMVKDR VACSVAYLED IETACDQVDN VIRDIYKYSK
210 220 230 240 250
PGYIFVPADF ADMSVTCDNL VNVPRISQQD CIVYPSENQL SDIINKITSW
260 270 280 290 300
IYSSKTPAIL GDVLTDRYGV SNFLNKLICK TGIWNFSTVM GKSVIDESNP
310 320 330 340 350
TYMGQYNGKE GLKQVYEHFE LCDLVLHFGV DINEINNGHY TFTYKPNAKI
360 370 380 390 400
IQFHPNYIRL VDTRQGNEQM FKGINFAPIL KELYKRIDVS KLSLQYDSNV
410 420 430 440 450
TQYTNETMRL EDPTNGQSSI ITQVHLQKTM PKFLNPGDVV VCETGSFQFS
460 470 480 490 500
VRDFAFPSQL KYISQGFFLS IGMALPAALG VGIAMQDHSN AHINGGNVKE
510 520 530 540 550
DYKPRLILFE GDGAAQMTIQ ELSTILKCNI PLEVIIWNNN GYTIERAIMG
560 570 580 590 600
PTRSYNDVMS WKWTKLFEAF GDFDGKYTNS TLIQCPSKLA LKLEELKNSN
610 620 630
KRSGIELLEV KLGELDFPEQ LKCMVEAAAL KRNKK
Length:635
Mass (Da):71,384
Last modified:November 1, 1996 - v1
Checksum:i616DF7E06DA82415
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28373 Genomic DNA. Translation: AAB64816.1.
BK006938 Genomic DNA. Translation: DAA12223.1.
PIRiS61175.
RefSeqiNP_010668.3. NM_001180688.3.

Genome annotation databases

EnsemblFungiiYDR380W; YDR380W; YDR380W.
GeneIDi851987.
KEGGisce:YDR380W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28373 Genomic DNA. Translation: AAB64816.1.
BK006938 Genomic DNA. Translation: DAA12223.1.
PIRiS61175.
RefSeqiNP_010668.3. NM_001180688.3.

3D structure databases

ProteinModelPortaliQ06408.
SMRiQ06408. Positions 23-630.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32440. 14 interactions.
DIPiDIP-5252N.
IntActiQ06408. 2 interactions.
MINTiMINT-546662.

Proteomic databases

MaxQBiQ06408.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR380W; YDR380W; YDR380W.
GeneIDi851987.
KEGGisce:YDR380W.

Organism-specific databases

EuPathDBiFungiDB:YDR380W.
SGDiS000002788. ARO10.

Phylogenomic databases

GeneTreeiENSGT00550000075465.
HOGENOMiHOG000061334.
InParanoidiQ06408.
KOiK12732.
OMAiRSYNDIQ.
OrthoDBiEOG092C2L8F.

Enzyme and pathway databases

UniPathwayiUPA00866.
BioCyciMetaCyc:MONOMER-11837.
YEAST:MONOMER3O-398.
BRENDAi4.1.1.43. 984.

Miscellaneous databases

PROiQ06408.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR012110. TPP_enzyme.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF036565. Pyruvt_ip_decrb. 1 hit.
SUPFAMiSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiARO10_YEAST
AccessioniPrimary (citable) accession number: Q06408
Secondary accession number(s): D6VT13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6560 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.