ID   3O1D_COMTE              Reviewed;         573 AA.
AC   Q06401;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   16-JUN-2009, entry version 44.
DE   RecName: Full=3-oxosteroid 1-dehydrogenase;
DE            EC=1.3.99.4;
OS   Comamonas testosteroni (Pseudomonas testosteroni).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Comamonas.
OX   NCBI_TaxID=285;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 17410;
RX   MEDLINE=92041619; PubMed=1657885;
RA   Plesiat P., Grandguillot M., Harayama S., Vragar S., Michel-Briand Y.;
RT   "Cloning, sequencing, and expression of the Pseudomonas testosteroni
RT   gene encoding 3-oxosteroid delta 1-dehydrogenase.";
RL   J. Bacteriol. 173:7219-7227(1991).
CC   -!- FUNCTION: Dehydrogenates steroids by introducing a double bond in
CC       steroid ring A.
CC   -!- CATALYTIC ACTIVITY: A 3-oxosteroid + acceptor = a 3-oxo-Delta(1)-
CC       steroid + reduced acceptor.
CC   -!- COFACTOR: FAD.
CC   -!- PATHWAY: Lipid metabolism; steroid degradation.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane
CC       protein.
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DR   EMBL; M68488; AAA25679.1; -; Genomic_DNA.
DR   PIR; A41319; A41319.
DR   BRENDA; 1.3.99.4; 4566.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0047571; F:3-oxosteroid 1-dehydrogenase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0008202; P:steroid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR003953; FAD_bind2_N.
DR   Pfam; PF00890; FAD_binding_2; 1.
PE   4: Predicted;
KW   Cell inner membrane; Cell membrane; FAD; Flavoprotein;
KW   Lipid metabolism; Membrane; Oxidoreductase; Steroid metabolism.
FT   CHAIN         1    573       3-oxosteroid 1-dehydrogenase.
FT                                /FTId=PRO_0000064376.
FT   NP_BIND       7     36       FAD (By similarity).
SQ   SEQUENCE   573 AA;  62673 MW;  8E941AF569897734 CRC64;
     MAEQEYDLIV VGSGAGACWA PIRAQEQGLK TLVVEKTELF GGTSALSGGG IWIPLNYDQK
     TAGIKDDLET AFGYMKRCVR GMATDDRVLA YVETASKMAE YLRQIGIPYR AMAKYADYYP
     HIEGSRPGGR TMDPVDFNAA RLRVTALETM RPGPPGNQLF GRMSISAFEA HSMLSRELKS
     RFTILGIMLK YFLDYPWRNK TRRDRRMTGG QALVAGLLTA ANKARVEMWC NSPLKELVQD
     ASGRVTGVIV ERNGQRQQIN ARRGVLLGAG GFERNQEMRD QYLNKPTRLV DGNPCGRQYG
     DAHRAGQAWA HTGADGLVLG RAHHGCSQGA GLSRHFRGTL AAGVHGGQRQ GAALPQRVRP
     VSGIPAAMLA ENAKGNGGVP AWIVFDASFR AQNPMGPLMP GSAVPDSKVR KSWLNNVYWK
     GRRWKIWRAD RRGRAGLQVS ARRMTEYARA GKDLDFDRGG NVFDRYYGDP RLKNPNLGPI
     EKGPFYAMRL WPGEIGTKGG LLTDREGRVL DTQGRIIEGL YCVGNNSASV MAPAYAGAGS
     TLGPAMTFAF RAVADMVGKP LPLENPHLLG KTV
//