Reviewed, UniProtKB/Swiss-Prot Q06401 (3O1D_COMTE)

Names and origin

Protein names	Recommended name:     3-oxosteroid 1-dehydrogenase     EC=1.3.99.4
Organism	Comamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifier	285 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Dehydrogenates steroids by introducing a double bond in steroid ring A.
Catalytic activity	A 3-oxosteroid + acceptor = a 3-oxo-Delta(1)-steroid + reduced acceptor.
Cofactor	FAD.
Pathway	Lipid metabolism; steroid degradation.
Subcellular location	Cell inner membrane; Peripheral membrane protein.

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxosteroid 1-dehydrogenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	3-oxosteroid 1-dehydrogenase		PRO_0000064376
Regions
	Nucleotide binding	7 – 36	30	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06401-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 8E941AF569897734	FASTA	573	62,673
	10 20 30 40 50 60 MAEQEYDLIV VGSGAGACWA PIRAQEQGLK TLVVEKTELF GGTSALSGGG IWIPLNYDQK 70 80 90 100 110 120 TAGIKDDLET AFGYMKRCVR GMATDDRVLA YVETASKMAE YLRQIGIPYR AMAKYADYYP 130 140 150 160 170 180 HIEGSRPGGR TMDPVDFNAA RLRVTALETM RPGPPGNQLF GRMSISAFEA HSMLSRELKS 190 200 210 220 230 240 RFTILGIMLK YFLDYPWRNK TRRDRRMTGG QALVAGLLTA ANKARVEMWC NSPLKELVQD 250 260 270 280 290 300 ASGRVTGVIV ERNGQRQQIN ARRGVLLGAG GFERNQEMRD QYLNKPTRLV DGNPCGRQYG 310 320 330 340 350 360 DAHRAGQAWA HTGADGLVLG RAHHGCSQGA GLSRHFRGTL AAGVHGGQRQ GAALPQRVRP 370 380 390 400 410 420 VSGIPAAMLA ENAKGNGGVP AWIVFDASFR AQNPMGPLMP GSAVPDSKVR KSWLNNVYWK 430 440 450 460 470 480 GRRWKIWRAD RRGRAGLQVS ARRMTEYARA GKDLDFDRGG NVFDRYYGDP RLKNPNLGPI 490 500 510 520 530 540 EKGPFYAMRL WPGEIGTKGG LLTDREGRVL DTQGRIIEGL YCVGNNSASV MAPAYAGAGS 550 560 570 TLGPAMTFAF RAVADMVGKP LPLENPHLLG KTV

References

[1]

"Cloning, sequencing, and expression of the Pseudomonas testosteroni gene encoding 3-oxosteroid delta 1-dehydrogenase." Plesiat P., Grandguillot M., Harayama S., Vragar S., Michel-Briand Y. J. Bacteriol. 173:7219-7227(1991) [PubMed: 1657885] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17410.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M68488 Genomic DNA. Translation: AAA25679.1.
PIR	A41319.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.99.4. 4566.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

3O1D_COMTE

Accession

Primary (citable) accession number: Q06401

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)