ID   GSTU6_ORYSJ             Reviewed;         236 AA.
AC   Q06398; B7E411; Q0IW60; Q9FUE6;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 2.
DT   24-JAN-2024, entry version 135.
DE   RecName: Full=Probable glutathione S-transferase GSTU6;
DE            EC=2.5.1.18;
DE   AltName: Full=28 kDa cold-induced protein;
GN   Name=GSTU6; OrderedLocusNames=Os10g0530900, LOC_Os10g38740;
GN   ORFNames=OSJNBb0038A07.2;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Nipponbare; TISSUE=Seedling;
RX   PubMed=16668981; DOI=10.1104/pp.99.3.1146;
RA   Binh L.T., Oono K.;
RT   "Molecular cloning and characterization of genes related to chilling
RT   tolerance in rice.";
RL   Plant Physiol. 99:1146-1150(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND NOMENCLATURE.
RX   PubMed=15069639; DOI=10.1007/s00438-004-1006-8;
RA   Soranzo N., Sari Gorla M., Mizzi L., De Toma G., Frova C.;
RT   "Organisation and structural evolution of the rice glutathione S-
RT   transferase gene family.";
RL   Mol. Genet. Genomics 271:511-521(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12791992; DOI=10.1126/science.1083523;
RA   Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA   Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA   Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA   Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA   Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA   Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA   Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA   Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA   Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA   Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA   Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA   Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA   Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA   Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA   Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA   Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA   Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT   "In-depth view of structure, activity, and evolution of rice chromosome
RT   10.";
RL   Science 300:1566-1569(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [6]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Conjugation of reduced glutathione to a wide number of
CC       exogenous and endogenous hydrophobic electrophiles.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glutathione + RX = a halide anion + an S-substituted
CC         glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC         ChEBI:CHEBI:90779; EC=2.5.1.18;
CC   -!- TISSUE SPECIFICITY: Expressed in seedling shoots and roots.
CC   -!- INDUCTION: By cold stress.
CC   -!- SIMILARITY: Belongs to the GST superfamily. Tau family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA01632.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; D10861; BAA01632.1; ALT_FRAME; mRNA.
DR   EMBL; AF309376; AAG32469.1; -; mRNA.
DR   EMBL; AC113948; AAM94535.1; -; Genomic_DNA.
DR   EMBL; DP000086; AAP54769.1; -; Genomic_DNA.
DR   EMBL; AP008216; BAF27055.1; -; Genomic_DNA.
DR   EMBL; AP014966; BAT11780.1; -; Genomic_DNA.
DR   EMBL; AK059760; BAG87108.1; -; mRNA.
DR   EMBL; AK103699; BAG96210.1; -; mRNA.
DR   RefSeq; XP_015614356.1; XM_015758870.1.
DR   AlphaFoldDB; Q06398; -.
DR   SMR; Q06398; -.
DR   STRING; 39947.Q06398; -.
DR   PaxDb; 39947-Q06398; -.
DR   EnsemblPlants; Os10t0530900-01; Os10t0530900-01; Os10g0530900.
DR   GeneID; 4349207; -.
DR   Gramene; Os10t0530900-01; Os10t0530900-01; Os10g0530900.
DR   KEGG; osa:4349207; -.
DR   eggNOG; KOG0406; Eukaryota.
DR   HOGENOM; CLU_011226_18_0_1; -.
DR   InParanoid; Q06398; -.
DR   OMA; KVRSAWL; -.
DR   OrthoDB; 451152at2759; -.
DR   Proteomes; UP000000763; Chromosome 10.
DR   Proteomes; UP000059680; Chromosome 10.
DR   ExpressionAtlas; Q06398; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004364; F:glutathione transferase activity; IBA:GO_Central.
DR   GO; GO:0006749; P:glutathione metabolic process; IBA:GO_Central.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   CDD; cd03185; GST_C_Tau; 1.
DR   CDD; cd03058; GST_N_Tau; 1.
DR   Gene3D; 1.20.1050.10; -; 1.
DR   Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR   InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR   InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR   InterPro; IPR040079; Glutathione_S-Trfase.
DR   InterPro; IPR004045; Glutathione_S-Trfase_N.
DR   InterPro; IPR045074; GST_C_Tau.
DR   InterPro; IPR045073; Omega/Tau-like.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR11260:SF326; GLUTATHIONE S-TRANSFERASE GSTU6-RELATED; 1.
DR   PANTHER; PTHR11260; GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING; 1.
DR   Pfam; PF13410; GST_C_2; 1.
DR   Pfam; PF13417; GST_N_3; 1.
DR   SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR   SFLD; SFLDG01152; Main.3:_Omega-_and_Tau-like; 1.
DR   SUPFAM; SSF47616; GST C-terminal domain-like; 1.
DR   SUPFAM; SSF52833; Thioredoxin-like; 1.
DR   PROSITE; PS50405; GST_CTER; 1.
DR   PROSITE; PS50404; GST_NTER; 1.
DR   Genevisible; Q06398; OS.
PE   2: Evidence at transcript level;
KW   Reference proteome; Stress response; Transferase.
FT   CHAIN           1..236
FT                   /note="Probable glutathione S-transferase GSTU6"
FT                   /id="PRO_0000185870"
FT   DOMAIN          5..84
FT                   /note="GST N-terminal"
FT   DOMAIN          94..228
FT                   /note="GST C-terminal"
FT   BINDING         15
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         42
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         56
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         68..69
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        65
FT                   /note="Missing (in Ref. 1; BAA01632)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        83..88
FT                   /note="GAGGRP -> RAGR (in Ref. 1; BAA01632)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        99..103
FT                   /note="AVARF -> VL (in Ref. 1; BAA01632)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        118
FT                   /note="Missing (in Ref. 1; BAA01632)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        125
FT                   /note="T -> M (in Ref. 1; BAA01632)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        135..143
FT                   /note="QAVAALETL -> RRGGVETV (in Ref. 1; BAA01632)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        158..162
FT                   /note="GGDGV -> ARRR (in Ref. 1; BAA01632)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        168
FT                   /note="V -> VV (in Ref. 1; BAA01632)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        184
FT                   /note="Missing (in Ref. 1; BAA01632)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        194..195
FT                   /note="PA -> SS (in Ref. 1; BAA01632)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        203..216
FT                   /note="FRATDAAKGVVPDD -> STHRLQCVDVPMT (in Ref. 1;
FT                   BAA01632)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   236 AA;  25656 MW;  60A5299744CB1F6C CRC64;
     MAGSGELKLL GVWSSPYAIR VRVVLNLKSL PYEYVEENLG DKSDLLLASN PVHKSVPVLL
     HAGRPVNESQ VIVQYIDEVW PGGAGGRPSV MPSDPYERAV ARFWAAYVDD KVRPAWLAIL
     FGSKTEEERA AAVAQAVAAL ETLEGAFGEC SKGKPFFGGD GVGFVDVVLG GYLGWFTAID
     KLIGRRLIDP ARTPALAAWE ERFRATDAAK GVVPDDADKL LEFRQTLLRW SASKAK
//