Q06398 (GSTU6_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutathione S-transferase GSTU6
EC=2.5.1.18

Alternative name(s):
28 kDa cold-induced protein

Gene names

Name:	GSTU6
Ordered Locus Names:	Os10g0530900, LOC_Os10g38740
ORF Names:	OSJNBb0038A07.2

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Tissue specificity	Expressed in seedling shoots and roots.
Induction	By cold stress.
Sequence similarities	Belongs to the GST superfamily. Tau family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence BAA01632.1 differs from that shown. Reason: Frameshift at positions 66, 86, 100, 119 and 185.

Ontologies

Keywords
Biological process	Stress response
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	de-etiolation Inferred from electronic annotation. Source: EnsemblPlants/Gramene lateral root development Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to cadmium ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to growth hormone stimulus Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to karrikin Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	Probable glutathione S-transferase GSTU6		PRO_0000185870
Regions
	Domain	5 – 84	80	GST N-terminal
	Domain	94 – 228	135	GST C-terminal
	Region	68 – 69	2	Glutathione binding By similarity
Sites
	Binding site	15	1	Glutathione By similarity
	Binding site	42	1	Glutathione By similarity
	Binding site	56	1	Glutathione; via amide nitrogen and carbonyl oxygen By similarity
Experimental info
	Sequence conflict	65	1	Missing in BAA01632. Ref.1
	Sequence conflict	83 – 88	6	GAGGRP → RAGR in BAA01632. Ref.1
	Sequence conflict	99 – 103	5	AVARF → VL in BAA01632. Ref.1
	Sequence conflict	118	1	Missing in BAA01632. Ref.1
	Sequence conflict	125	1	T → M in BAA01632. Ref.1
	Sequence conflict	135 – 143	9	QAVAALETL → RRGGVETV in BAA01632. Ref.1
	Sequence conflict	158 – 162	5	GGDGV → ARRR in BAA01632. Ref.1
	Sequence conflict	168	1	V → VV in BAA01632. Ref.1
	Sequence conflict	184	1	Missing in BAA01632. Ref.1
	Sequence conflict	194 – 195	2	PA → SS in BAA01632. Ref.1
	Sequence conflict	203 – 216	14	FRATD…VVPDD → STHRLQCVDVPMT in BAA01632. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q06398 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: 60A5299744CB1F6C	FASTA	236	25,656
	10 20 30 40 50 60 MAGSGELKLL GVWSSPYAIR VRVVLNLKSL PYEYVEENLG DKSDLLLASN PVHKSVPVLL 70 80 90 100 110 120 HAGRPVNESQ VIVQYIDEVW PGGAGGRPSV MPSDPYERAV ARFWAAYVDD KVRPAWLAIL 130 140 150 160 170 180 FGSKTEEERA AAVAQAVAAL ETLEGAFGEC SKGKPFFGGD GVGFVDVVLG GYLGWFTAID 190 200 210 220 230 KLIGRRLIDP ARTPALAAWE ERFRATDAAK GVVPDDADKL LEFRQTLLRW SASKAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of genes related to chilling tolerance in rice." Binh L.T., Oono K. Plant Physiol. 99:1146-1150(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. Tissue: Seedling.
[2]	"Organisation and structural evolution of the rice glutathione S-transferase gene family." Soranzo N., Sari Gorla M., Mizzi L., De Toma G., Frova C. Mol. Genet. Genomics 271:511-521(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE.
[3]	"In-depth view of structure, activity, and evolution of rice chromosome 10." Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H. , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A. Science 300:1566-1569(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10861 mRNA. Translation: BAA01632.1. Frameshift. AF309376 mRNA. Translation: AAG32469.1. AC113948 Genomic DNA. Translation: AAM94535.1. DP000086 Genomic DNA. Translation: AAP54769.1. AP008216 Genomic DNA. Translation: BAF27055.1.
RefSeq	NP_001065141.1. NM_001071676.1.
UniGene	Os.4762.
3D structure databases
ProteinModelPortal	Q06398.
SMR	Q06398. Positions 5-225.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os10g38740.1; LOC_Os10g38740.1; LOC_Os10g38740.
GeneID	4349207.
KEGG	osa:4349207.
Organism-specific databases
Gramene	Q06398.
Phylogenomic databases
eggNOG	NOG271594.
HOGENOM	HOG000125749.
KO	K00799.
OMA	VAYEYLE.
ProtClustDB	CLSN2698219.
Gene expression databases
ArrayExpress	Q06398.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTU6_ORYSJ

Accession

Primary (citable) accession number: Q06398
Secondary accession number(s): Q0IW60, Q9FUE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 4, 2005
Last modified:	April 3, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program