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Protein

Calcium-binding protein NCS-1

Gene

FRQ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of phosphatidylinositol 4-kinase PIK1.1 Publication

Miscellaneous

Binds 3 calcium ions via the second, third and fourth EF-hand.
Present with 7160 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi73 – 841Add BLAST12
Calcium bindingi109 – 1202Add BLAST12
Calcium bindingi157 – 1683Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: SGD
  • enzyme activator activity Source: SGD

GO - Biological processi

  • regulation of signal transduction Source: SGD

Keywordsi

LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29923-MONOMER.
ReactomeiR-SCE-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding protein NCS-1
Gene namesi
Name:FRQ1
Synonyms:NCS1
Ordered Locus Names:YDR373W
ORF Names:D9481.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR373W.
SGDiS000002781. FRQ1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Abolishes N-myristoylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000737952 – 190Calcium-binding protein NCS-1Add BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiQ06389.
PRIDEiQ06389.

PTM databases

iPTMnetiQ06389.

Interactioni

Subunit structurei

Binds PIK1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32432. 280 interactors.
DIPiDIP-2733N.
IntActiQ06389. 38 interactors.
MINTiMINT-513362.
STRINGi4932.YDR373W.

Structurei

Secondary structure

1190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 10Combined sources4
Helixi11 – 16Combined sources6
Turni17 – 19Combined sources3
Helixi24 – 37Combined sources14
Helixi45 – 55Combined sources11
Helixi63 – 70Combined sources8
Beta strandi77 – 80Combined sources4
Helixi82 – 92Combined sources11
Helixi99 – 108Combined sources10
Beta strandi113 – 117Combined sources5
Helixi118 – 129Combined sources12
Beta strandi137 – 139Combined sources3
Helixi145 – 156Combined sources12
Turni157 – 159Combined sources3
Beta strandi161 – 165Combined sources5
Helixi166 – 174Combined sources9
Helixi179 – 187Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FPWNMR-A1-190[»]
2JU0NMR-A1-190[»]
ProteinModelPortaliQ06389.
SMRiQ06389.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06389.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 59EF-hand 1Add BLAST36
Domaini60 – 95EF-hand 2Add BLAST36
Domaini96 – 131EF-hand 3Add BLAST36
Domaini144 – 179EF-hand 4Add BLAST36

Sequence similaritiesi

Belongs to the recoverin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
InParanoidiQ06389.
KOiK19932.
OMAiKRELQQW.
OrthoDBiEOG092C4XFN.

Family and domain databases

CDDicd00051. EFh. 2 hits.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
PfamiView protein in Pfam
PF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 3 hits.
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAKTSKLSK DDLTCLKQST YFDRREIQQW HKGFLRDCPS GQLAREDFVK
60 70 80 90 100
IYKQFFPFGS PEDFANHLFT VFDKDNNGFI HFEEFITVLS TTSRGTLEEK
110 120 130 140 150
LSWAFELYDL NHDGYITFDE MLTIVASVYK MMGSMVTLNE DEATPEMRVK
160 170 180 190
KIFKLMDKNE DGYITLDEFR EGSKVDPSII GALNLYDGLI
Length:190
Mass (Da):22,011
Last modified:January 23, 2007 - v2
Checksum:i5E9D7F48F8AADA21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28373 Genomic DNA. Translation: AAB64809.1.
BK006938 Genomic DNA. Translation: DAA12214.1.
PIRiS61168.
RefSeqiNP_010661.3. NM_001180681.3.

Genome annotation databases

EnsemblFungiiYDR373W; YDR373W; YDR373W.
GeneIDi851979.
KEGGisce:YDR373W.

Similar proteinsi

Entry informationi

Entry nameiNCS1_YEAST
AccessioniPrimary (citable) accession number: Q06389
Secondary accession number(s): D6VT04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names