Reviewed, UniProtKB/Swiss-Prot Q06378 (GLNA3_HORVU)

Names and origin

Protein names	Recommended name:     Glutamine synthetase     EC=6.3.1.2 Alternative name(s):     Cytoplasmic GS3     Glutamate--ammonia ligase
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
Subunit structure	Homooctamer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	In barley, there are distinct isozymes in the chloroplast, and cytoplasm.
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Glutamine synthetase		PRO_0000153173

Sequences

	Sequence		Length	Mass (Da)
	Q06378-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 541DB976BFF04BC5	FASTA	356	39,128
	10 20 30 40 50 60 MALLTDLLNL DLSGSTEKII AEYIWIGGSG MDLRSKARHL PGPVTHPSKL PKWNYDGSST 70 80 90 100 110 120 GQAPGEDSEV ILYPQAILKD PFREGNNILV MCDCYTPRGE PIPTNKRYNA AKILSNPDVA 130 140 150 160 170 180 KEEPWYGIEQ EYTLLQKDIN WPLGWPVGGF PGPQGPYYCG IGADKSFGRD IVDSHYKACL 190 200 210 220 230 240 FGGVNISGIN GEVMPGQWEF QVGPTVGISA GDQVWVARYI LERITEIAGV VVTFDPKPIP 250 260 270 280 290 300 GDWNGAGAHT NYSTESMRND GGFKVIVDAV EKLKLKHKEH IAAYGEGNER RLTGKHETAD 310 320 330 340 350 INTSSWGVAN RGASVRVGRE TEQNGKGYFE DRRPASNMDP YVVTSMIAQT TILWKP

References

[1]	"Isolation and characterization of a cDNA coding for cytoplasmic glutamine synthetase of barley." Marigo C., Zito F., Casadoro G. Hereditas 118:281-284(1993) [PubMed: 8104170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X69087 mRNA. Translation: CAA48830.1.
UniGene	Hv.331
3D structure databases
SMR	Q06378. Positions 4-355.
ModBase	Search...
Organism-specific databases
Gramene	Q06378.
Enzyme and pathway databases
BRENDA	6.3.1.2. 283.
Family and domain databases
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat. IPR008146. Gln_synth_cat. [Graphical view]
Gene3D	G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
ProDom	PD001057. Gln_synt_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNA3_HORVU

Accession

Primary (citable) accession number: Q06378

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)