Reviewed, UniProtKB/Swiss-Prot Q06367 (CP1A1_CAVPO)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 1A1
    EC=1.14.14.1
Alternative name(s):
    CYPIA1

Gene names

Name:

CYP1A1

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Constitutively expressed in liver.
Induction	By 3-methylcholanthrene (3MC).
Miscellaneous	Constitutive expression of this enzyme in liver may be related to the sensitivity of guinea pig to TCDD.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 516	516	Cytochrome P450 1A1		PRO_0000051626
Sites
	Metal binding	453	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	2	1	Missing AA sequence Ref.2
	Sequence conflict	16	1	C → W AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q06367-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 026DD4DCAB6E39BA	FASTA	516	58,571
	10 20 30 40 50 60 MSTSAMELLL TATIFCLVLW VVRIFRPQVP KGLKSPPGPW GWPLIGHMLT LGKNPHLALT 70 80 90 100 110 120 RLSARYGDVL QIRIGSTPVV VLSGLDTIRQ ALVRQGDDFK GRPDLYSFTF ISDGQSMTFN 130 140 150 160 170 180 PDSGPVWAAR RRLAQSALKS FSVASDPASV SSCYLEEHVK KEAEYLIKKF QELMAGPGHF 190 200 210 220 230 240 DPYRYVVVSV ANVISAICFG QRYSHDDQQL LELIDLNNEF GEVTGSGNPS DFIPILRYLP 250 260 270 280 290 300 SATMDTFKDL NRRFSVFIQK MIKEHYKTFE KGHIRDITDS LIEHCQDRKL DKNANIQISD 310 320 330 340 350 360 QKIIGIVLDL FGAGFDTITT AISWSLLYLV MNPRIQKKIQ EELDTVIGRE RQPQLADRPK 370 380 390 400 410 420 LPYMEAFISE VFRYSSFMPF TIPHSTTKDT SLNGFYIPKG CCIFVNQWQI NHDQKLWGDP 430 440 450 460 470 480 SVFRPERFLS PDGTVDKALS EKVTIFGLGK RRCLGEVIGR WEVFLFLAIL LQQLEFSTSP 490 500 510 GVKIDMTPIY GLTMKYSRCE HFQAQTRPFV LKCPEA

References

[1]	"Molecular cloning of guinea pig CYP1A1: complete primary structure and fast mobility of expressed protein on electrophoresis." Ohgiya S., Ishizaki K., Shinriki N. Biochim. Biophys. Acta 1216:237-244(1993) [PubMed: 8241264] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley. Tissue: Liver.
[2]	"Amino-terminal sequence and structure of monoclonal antibody immunopurified cytochromes P-450." Cheng K.C., Park S.S., Krutzsch H.C., Grantham P.H., Gelboin H.V., Friedman F.K. Biochemistry 25:2397-2402(1986) [PubMed: 3718958] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-24. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11043 mRNA. Translation: BAA01794.1.
PIR	S43414.
3D structure databases
SMR	Q06367. Positions 28-505.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCPOT00000015748; ENSCPOP00000014064; ENSCPOG00000015596; Cavia porcellus. [Genome view]
Phylogenomic databases
eggNOG	roNOG06549.
HOVERGEN	Q06367.
InParanoid	Q06367.
OMA	CEHFQAQ.
OrthoDB	EOG95MQQK.
Enzyme and pathway databases
BRENDA	1.14.14.1. 44.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit. PTHR19383:SF63. Cyt_P450_E_grp-I_CYP1. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP1A1_CAVPO

Accession

Primary (citable) accession number: Q06367

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)