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Protein

Inositol phosphorylceramide synthase regulatory subunit KEI1

Gene

KEI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory component of the inositol phosphorylceramide (ICP) synthase which catalyzes the addition of a phosphorylinositol group onto ceramide to form inositol phosphorylceramide, an essential step in sphingolipid biosynthesis. Helps the medial Golgi localization of IPC synthase in a COPI vesicle-dependent manner.1 Publication

Miscellaneous

Present with 3420 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • inositol phosphoceramide synthase regulator activity Source: SGD

GO - Biological processi

  • inositolphosphoceramide metabolic process Source: SGD

Keywordsi

Biological processLipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:G3O-29917-MONOMER.
YEAST:G3O-29917-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol phosphorylceramide synthase regulatory subunit KEI1
Short name:
ICP synthase regulatory subunit KEI1
Alternative name(s):
KEX2-cleavable protein essential for inositol phosphorylceramide synthesis
Cleaved into the following 2 chains:
Gene namesi
Name:KEI1
Ordered Locus Names:YDR367W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR367W.
SGDiS000002775. KEI1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei11 – 31HelicalSequence analysisAdd BLAST21
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Transmembranei79 – 99HelicalSequence analysisAdd BLAST21
Transmembranei154 – 174HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • inositol phosphoceramide synthase complex Source: SGD
  • integral component of Golgi membrane Source: SGD

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi103F → I: Decreases ICP synthase activity. 1 Publication1
Mutagenesisi135R → S: Impairs cleavage by KEX2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002538461 – 221Inositol phosphorylceramide synthase regulatory subunit KEI1Add BLAST221
ChainiPRO_00004094451 – 135KEI1NAdd BLAST135
ChainiPRO_0000409446136 – 221KEI1CAdd BLAST86

Post-translational modificationi

The precursor protein is cleaved into two polypeptide chains, KEI1N and KEI1C. The cleavage is performed in the Golgi apparatus by the KEX2 protease which recognizes residue Arg-135. Generation of KEX2 cleavage site may have been an accidental event in evolution without specific advantages or disadvantages in IPC synthesis.

Proteomic databases

MaxQBiQ06346.
PRIDEiQ06346.

Interactioni

Subunit structurei

Component of the inositol phosphorylceramide synthase complex composed of at least AUR1 and KEI1. Interacts (via C-terminal region) with COP1 and SEC21. Note=The interaction with AUR1 seems to occur with the full length protein before cleavage by KEX2 since both full length and short chains of KEI1 interact with AUR1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AUR1P361072EBI-38431,EBI-2046036

Protein-protein interaction databases

BioGridi32426. 15 interactors.
DIPiDIP-8823N.
IntActiQ06346. 5 interactors.
MINTiMINT-518732.

Structurei

3D structure databases

ProteinModelPortaliQ06346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni176 – 221COPI vesicle-bindingAdd BLAST46

Sequence similaritiesi

Belongs to the KEI1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ06346.
OMAiQLMILEG.
OrthoDBiEOG092C55T5.

Family and domain databases

InterProiView protein in InterPro
IPR013862. DUF1753_Golgi.
PfamiView protein in Pfam
PF08552. Kei1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06346-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSSLLTLPK SFLGFMPLYL AVEIVLGISI LNKCSGAYGI LALFTGHPLD
60 70 80 90 100
FMQWIAYLWS VFTLIVFSQG LYLIHKPNLL VFSQICVLYT IDTISTCFFT
110 120 130 140 150
LWFTTQWFTL EDTANIDGNN ALQSNPISTG KLTERGIDIS KQSATESYEY
160 170 180 190 200
TMTILITLVS LIFRFYFNFI LASFVQELLH HPKYLVDRDD VEQNLKNKPI
210 220
WKRLWAKSQK GCYKLCKNLL E
Length:221
Mass (Da):25,484
Last modified:October 17, 2006 - v2
Checksum:i4EC6A3FF541FC6F5
GO

Sequence cautioni

The sequence AAB64803 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA56685 differs from that shown. Reason: Frameshift at positions 11 and 175.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28373 Genomic DNA. Translation: AAB64803.1. Sequence problems.
X80642 Genomic DNA. Translation: CAA56685.1. Frameshift.
BK006938 Genomic DNA. Translation: DAA12208.1.
PIRiS70234.
RefSeqiNP_010655.1. NM_001180675.1.

Genome annotation databases

EnsemblFungiiYDR367W; YDR367W; YDR367W.
GeneIDi851973.
KEGGisce:YDR367W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28373 Genomic DNA. Translation: AAB64803.1. Sequence problems.
X80642 Genomic DNA. Translation: CAA56685.1. Frameshift.
BK006938 Genomic DNA. Translation: DAA12208.1.
PIRiS70234.
RefSeqiNP_010655.1. NM_001180675.1.

3D structure databases

ProteinModelPortaliQ06346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32426. 15 interactors.
DIPiDIP-8823N.
IntActiQ06346. 5 interactors.
MINTiMINT-518732.

Proteomic databases

MaxQBiQ06346.
PRIDEiQ06346.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR367W; YDR367W; YDR367W.
GeneIDi851973.
KEGGisce:YDR367W.

Organism-specific databases

EuPathDBiFungiDB:YDR367W.
SGDiS000002775. KEI1.

Phylogenomic databases

InParanoidiQ06346.
OMAiQLMILEG.
OrthoDBiEOG092C55T5.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-29917-MONOMER.
YEAST:G3O-29917-MONOMER.

Miscellaneous databases

PROiPR:Q06346.

Family and domain databases

InterProiView protein in InterPro
IPR013862. DUF1753_Golgi.
PfamiView protein in Pfam
PF08552. Kei1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKEI1_YEAST
AccessioniPrimary (citable) accession number: Q06346
Secondary accession number(s): D6VSZ8, Q06999
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 17, 2006
Last modified: June 7, 2017
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.