Q06340 (ESC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein ESC2

Alternative name(s):
Establishes silent chromatin protein 2

Gene names

Name:	ESC2
Ordered Locus Names:	YDR363W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in HMR and telomere silencing via the recruitment or stabilizing of the SIR (silent information regulators) complex. Ref.3 Ref.4
Subunit structure	Interacts with SIR2. Ref.4
Subcellular location	Cytoplasm. Nucleus Ref.3 Ref.5.
Domain	The 2 SUMO-like regions, although related to ubiquitin domains lack the conserved acceptor Gly residue in position 456. Ref.7
Miscellaneous	Present with 1890 molecules/cell in log phase SD medium. Ref.6

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Coiled coil Repeat
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chromatin silencing at silent mating-type cassette Inferred from mutant phenotype Ref.3. Source: SGD double-strand break repair via homologous recombination Inferred from mutant phenotype. Source: SGD intra-S DNA damage checkpoint Inferred from mutant phenotype. Source: SGD mitotic sister chromatid cohesion Inferred from mutant phenotype. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Protein ESC2		PRO_0000227693
Regions
	Repeat	169 – 287	119	SUMO-like region 1
	Repeat	380 – 456	77	SUMO-like region 2
	Coiled coil	301 – 360	60	Potential
Amino acid modifications
	Modified residue	149	1	Phosphoserine Ref.8
	Modified residue	151	1	Phosphoserine Ref.8
	Modified residue	379	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q06340 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F691FB73ED2FAF15	FASTA	456	52,542
	10 20 30 40 50 60 MTGDSRSISE PSINLDPDNT SFSDENSDDF FMDNSYDIDE IDHSDESNRQ SVIVDSKVTV 70 80 90 100 110 120 PPSKHSTLTL SDSEDSDAKE QHQSLSRSSS KNVNIEDITE PKPDKPSGRT RGRSVMKESV 130 140 150 160 170 180 VEINSSESDL DEDKNFPRSR SRSRSSIRSI SPAGKYKRQK SSLLYTYDEN DDFFKELAKE 190 200 210 220 230 240 AKKSTTISKE STPDQRKRVY NIKFLSKLEG TINKAVQVKV LGKYEFSKIL PAALDGLMKS 250 260 270 280 290 300 YKIPKVMKDI YKVENVTLYW NNAKLLTFMT CNSLHIPQDF ENEVSDIDVT IVSKEYEKNF 310 320 330 340 350 360 EATLESKLKE EEAALLIKER QEMERKLEKK RNEQEESEYR EFESELKNVE ETQEIKENDT 370 380 390 400 410 420 VMNTKLLQEG GSLSGNSSSM EEVMRIALMG QDNKKIYVHV RRSTPFSKIA EYYRIQKQLP 430 440 450 QKTRVKLLFD HDELDMNECI ADQDMEDEDM VDVIID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A histone variant, Htz1p, and a Sir1p-like protein, Esc2p, mediate silencing at HMR." Dhillon N., Kamakaka R.T. Mol. Cell 6:769-780(2000) [PubMed: 11090616] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"Restoration of silencing in Saccharomyces cerevisiae by tethering of a novel Sir2-interacting protein, Esc8." Cuperus G., Shore D. Genetics 162:633-645(2002) [PubMed: 12399377] [Abstract] Cited for: FUNCTION, INTERACTION WITH SIR2.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteins with two SUMO-like domains in chromatin-associated complexes: the RENi (Rad60-Esc2-NIP45) family." Novatchkova M., Bachmair A., Eisenhaber B., Eisenhaber F. BMC Bioinformatics 6:22-22(2005) [PubMed: 15698469] [Abstract] Cited for: DOMAIN.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149 AND SER-151, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-379, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28372 Genomic DNA. Translation: AAB64797.1. BK006938 Genomic DNA. Translation: DAA12201.1.
PIR	S61158.
RefSeq	NP_010650.1. NM_001180671.1.
3D structure databases
ProteinModelPortal	Q06340.
SMR	Q06340. Positions 380-456.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5219N.
IntAct	Q06340. 1 interaction.
MINT	MINT-510947.
STRING	Q06340.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR363W; YDR363W; YDR363W.
GeneID	851965.
KEGG	sce:YDR363W.
NMPDR	fig|4932.3.peg.1417.
Organism-specific databases
CYGD	YDR363w.
SGD	S000002771. ESC2.
Phylogenomic databases
eggNOG	fuNOG11723.
OMA	QPESEAN.
OrthoDB	EOG4J9R8F.
Gene expression databases
ArrayExpress	Q06340.
Genevestigator	Q06340.
GermOnline	YDR363W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022617. SUMO. IPR019955. Ubiquitin_supergroup. [Graphical view]
Pfam	PF11976. Rad60-SLD. 1 hit. [Graphical view]
PROSITE	PS50053. UBIQUITIN_2. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...
Other
NextBio	970080.

Entry information

Entry name

ESC2_YEAST

Accession

Primary (citable) accession number: Q06340
Secondary accession number(s): D6VSZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program