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Protein

Amyloid-like protein 2

Gene

Aplp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein (By similarity). Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular copper ion homeostasis Source: MGI
  • cholesterol metabolic process Source: MGI
  • extracellular matrix organization Source: MGI
  • forebrain development Source: MGI
  • locomotory behavior Source: MGI
  • mating behavior Source: MGI
  • midbrain development Source: MGI
  • neuromuscular process controlling balance Source: MGI
  • regulation of epidermal growth factor-activated receptor activity Source: MGI
  • regulation of protein binding Source: MGI
  • suckling behavior Source: MGI
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Amyloid-like protein 2
Short name:
APLP-2
Alternative name(s):
CDEI box-binding protein
Short name:
CDEBP
Gene namesi
Name:Aplp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88047. Aplp2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 636605ExtracellularSequence analysisAdd
BLAST
Transmembranei637 – 66024HelicalSequence analysisAdd
BLAST
Topological domaini661 – 70747CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Chaini32 – 707676Amyloid-like protein 2PRO_0000000208Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei216 – 2161PhosphoserineBy similarity
Glycosylationi485 – 4851N-linked (GlcNAc...)Sequence analysis
Modified residuei534 – 5341PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ06335.
PaxDbiQ06335.
PeptideAtlasiQ06335.
PRIDEiQ06335.

PTM databases

iPTMnetiQ06335.
PhosphoSiteiQ06335.

Expressioni

Gene expression databases

BgeeiQ06335.
CleanExiMM_APLP2.
ExpressionAtlasiQ06335. baseline and differential.
GenevisibleiQ06335. MM.

Interactioni

Subunit structurei

Interacts with CPEB1. Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AppP120233EBI-446708,EBI-78814

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198154. 3 interactions.
IntActiQ06335. 12 interactions.
MINTiMINT-1177305.
STRINGi10090.ENSMUSP00000072428.

Structurei

3D structure databases

ProteinModelPortaliQ06335.
SMRiQ06335. Positions 47-204, 331-512, 676-706.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni693 – 70715Interaction with DAB2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi694 – 6996NPXY motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi218 – 29477Asp/Glu-rich (highly acidic)Add
BLAST
Compositional biasi218 – 23114Poly-GluAdd
BLAST
Compositional biasi256 – 26611Poly-GluAdd
BLAST

Sequence similaritiesi

Belongs to the APP family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3540. Eukaryota.
ENOG410ZW2A. LUCA.
GeneTreeiENSGT00530000063252.
HOGENOMiHOG000232190.
HOVERGENiHBG000051.
InParanoidiQ06335.
KOiK08117.

Family and domain databases

Gene3Di3.30.1490.140. 1 hit.
3.90.570.10. 1 hit.
InterProiIPR008155. Amyloid_glyco.
IPR011178. Amyloid_glyco_Cu-bd.
IPR024329. Amyloid_glyco_E2_domain.
IPR008154. Amyloid_glyco_extra.
IPR019744. Amyloid_glyco_extracell_CS.
IPR015849. Amyloid_glyco_heparin-bd.
IPR019745. Amyloid_glyco_intracell_CS.
IPR019543. APP_amyloid_C.
[Graphical view]
PfamiPF10515. APP_amyloid. 1 hit.
PF12924. APP_Cu_bd. 1 hit.
PF12925. APP_E2. 1 hit.
PF02177. APP_N. 1 hit.
[Graphical view]
PRINTSiPR00203. AMYLOIDA4.
SMARTiSM00006. A4_EXTRA. 1 hit.
[Graphical view]
SUPFAMiSSF109843. SSF109843. 1 hit.
SSF56491. SSF56491. 1 hit.
SSF89811. SSF89811. 1 hit.
PROSITEiPS00319. A4_EXTRA. 1 hit.
PS00320. A4_INTRA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q06335-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATGTAAAA ATGKLLVLLL LGLTAPAAAL AGYIEALAAN AGTGFAVAEP
60 70 80 90 100
QIAMFCGKLN MHVNIQTGKW EPDPTGTKSC LGTKEEVLQY CQEIYPELQI
110 120 130 140 150
TNVMEANQPV NIDSWCRRDK RQCKSHIVIP FKCLVGEFVS DVLLVPDNCQ
160 170 180 190 200
FFHQERMEVC EKHQRWHTLV KEACLTEGLT LYSYGMLLPC GVDQFHGTEY
210 220 230 240 250
VCCPQTKTVD SDSTMSKEEE EEEEDEEDEE EDYDLDKSEF PTEADLEDFT
260 270 280 290 300
EAAADEEEED EEEGEEVVED RDYYYDPFKG DDYNEENPTE PSSEGTISDK
310 320 330 340 350
EIVHDVKVPP TPLPTNDVDV YFETSADDNE HARFQKAKEQ LEIRHRNRMD
360 370 380 390 400
RVKKEWEEAE LQAKNLPKTE RQTLIQHFQA MVKALEKEAA SEKQQLVETH
410 420 430 440 450
LARVEAMLND RRRIALENYL AALQSDPPRP HRILQALRRY VRAENKDRLH
460 470 480 490 500
TIRHYQHVLA VDPEKAAQMK SQVMTHLHVI EERRNQSLSL LYKVPYVAQE
510 520 530 540 550
IQEEIDELLQ EQRADMDQFT SSISENPVDV RVSSEESEEI PPFHPLHPFP
560 570 580 590 600
SLSENEDTQP ELYHPMKKGS GMAEQDGGLI GAEEKVINSK NKMDENMVID
610 620 630 640 650
ETLDVKEMIF NAERVGGLEE EPESVGPLRE DFSLSSNALI GLLVIAVAIA
660 670 680 690 700
TVIVISLVML RKRQYGTISH GIVEVDPMLT PEERHLNKMQ NHGYENPTYK

YLEQMQI
Length:707
Mass (Da):80,467
Last modified:June 28, 2011 - v4
Checksum:iBBB8C3E25A6CC503
GO
Isoform 2 (identifier: Q06335-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     557-568: Missing.

Show »
Length:695
Mass (Da):78,998
Checksum:iADB62430849EF6AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551F → L in CAA80306 (Ref. 1) Curated
Sequence conflicti185 – 1895GMLLP → MACCC in AAA20039 (PubMed:1482349).Curated
Sequence conflicti322 – 3221F → L in CAA80306 (Ref. 1) Curated
Sequence conflicti322 – 3221F → L in AAA20039 (PubMed:1482349).Curated
Sequence conflicti489 – 4891S → T in CAA80306 (Ref. 1) Curated
Sequence conflicti489 – 4891S → T in AAA20039 (PubMed:1482349).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei557 – 56812Missing in isoform 2. 2 PublicationsVSP_041386Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22592 mRNA. Translation: CAA80306.1.
BC051999 mRNA. Translation: AAH51999.1.
M97216 mRNA. Translation: AAA20039.1.
U34291 Genomic DNA. Translation: AAC52318.1.
CCDSiCCDS52748.1. [Q06335-1]
PIRiJC1404.
S38344.
RefSeqiNP_001095925.1. NM_001102455.1.
NP_001095926.1. NM_001102456.1. [Q06335-1]
UniGeneiMm.19133.

Genome annotation databases

EnsembliENSMUST00000079758; ENSMUSP00000078694; ENSMUSG00000031996. [Q06335-1]
GeneIDi11804.
KEGGimmu:11804.
UCSCiuc009orl.1. mouse. [Q06335-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22592 mRNA. Translation: CAA80306.1.
BC051999 mRNA. Translation: AAH51999.1.
M97216 mRNA. Translation: AAA20039.1.
U34291 Genomic DNA. Translation: AAC52318.1.
CCDSiCCDS52748.1. [Q06335-1]
PIRiJC1404.
S38344.
RefSeqiNP_001095925.1. NM_001102455.1.
NP_001095926.1. NM_001102456.1. [Q06335-1]
UniGeneiMm.19133.

3D structure databases

ProteinModelPortaliQ06335.
SMRiQ06335. Positions 47-204, 331-512, 676-706.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198154. 3 interactions.
IntActiQ06335. 12 interactions.
MINTiMINT-1177305.
STRINGi10090.ENSMUSP00000072428.

PTM databases

iPTMnetiQ06335.
PhosphoSiteiQ06335.

Proteomic databases

MaxQBiQ06335.
PaxDbiQ06335.
PeptideAtlasiQ06335.
PRIDEiQ06335.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079758; ENSMUSP00000078694; ENSMUSG00000031996. [Q06335-1]
GeneIDi11804.
KEGGimmu:11804.
UCSCiuc009orl.1. mouse. [Q06335-1]

Organism-specific databases

CTDi334.
MGIiMGI:88047. Aplp2.

Phylogenomic databases

eggNOGiKOG3540. Eukaryota.
ENOG410ZW2A. LUCA.
GeneTreeiENSGT00530000063252.
HOGENOMiHOG000232190.
HOVERGENiHBG000051.
InParanoidiQ06335.
KOiK08117.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Miscellaneous databases

ChiTaRSiAplp2. mouse.
PROiQ06335.
SOURCEiSearch...

Gene expression databases

BgeeiQ06335.
CleanExiMM_APLP2.
ExpressionAtlasiQ06335. baseline and differential.
GenevisibleiQ06335. MM.

Family and domain databases

Gene3Di3.30.1490.140. 1 hit.
3.90.570.10. 1 hit.
InterProiIPR008155. Amyloid_glyco.
IPR011178. Amyloid_glyco_Cu-bd.
IPR024329. Amyloid_glyco_E2_domain.
IPR008154. Amyloid_glyco_extra.
IPR019744. Amyloid_glyco_extracell_CS.
IPR015849. Amyloid_glyco_heparin-bd.
IPR019745. Amyloid_glyco_intracell_CS.
IPR019543. APP_amyloid_C.
[Graphical view]
PfamiPF10515. APP_amyloid. 1 hit.
PF12924. APP_Cu_bd. 1 hit.
PF12925. APP_E2. 1 hit.
PF02177. APP_N. 1 hit.
[Graphical view]
PRINTSiPR00203. AMYLOIDA4.
SMARTiSM00006. A4_EXTRA. 1 hit.
[Graphical view]
SUPFAMiSSF109843. SSF109843. 1 hit.
SSF56491. SSF56491. 1 hit.
SSF89811. SSF89811. 1 hit.
PROSITEiPS00319. A4_EXTRA. 1 hit.
PS00320. A4_INTRA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. von der Kammer H.
    Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Fetal brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
  3. "The complete cDNA coding sequence for the mouse CDEI binding protein."
    Hanes J., von der Kammer H., Kristjansson G.I., Scheit K.H.
    Biochim. Biophys. Acta 1216:154-156(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-246 (ISOFORMS 1/2).
  4. "A murine sequence-specific DNA binding protein shows extensive local similarities to the amyloid precursor protein."
    Vidal F., Blangy A., Rassoulzadegan M., Cuzin F.
    Biochem. Biophys. Res. Commun. 189:1336-1341(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 185-707 (ISOFORM 2).
    Strain: BALB/cJ.
    Tissue: Heart.
  5. "The mouse APLP2 gene. Chromosomal localization and promoter characterization."
    von Koch C.S., Lahiri D.K., Mammen A.L., Copeland N.G., Gilbert D.J., Jenkins N.A., Sisodia S.S.
    J. Biol. Chem. 270:25475-25480(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35.
    Strain: 129/Sv.
  6. "Disabled-2 colocalizes with the LDLR in clathrin-coated pits and interacts with AP-2."
    Morris S.M., Cooper J.A.
    Traffic 2:111-123(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DAB2.
  7. "The amyloid precursor protein (APP) of Alzheimer disease and its paralog, APLP2, modulate the Cu/Zn-nitric oxide-catalyzed degradation of glypican-1 heparan sulfate in vivo."
    Cappai R., Cheng F., Ciccotosto G.D., Needham B.E., Masters C.L., Multhaup G., Fransson L.A., Mani K.
    J. Biol. Chem. 280:13913-13920(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Amyloid precursor proteins anchor CPEB to membranes and promote polyadenylation-induced translation."
    Cao Q., Huang Y.-S., Kan M.-C., Richter J.D.
    Mol. Cell. Biol. 25:10930-10939(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CPEB1.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Lung and Testis.

Entry informationi

Entry nameiAPLP2_MOUSE
AccessioniPrimary (citable) accession number: Q06335
Secondary accession number(s): Q80US7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 28, 2011
Last modified: July 6, 2016
This is version 139 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.