Q06328 (YD352_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar integral membrane protein YDR352W

Gene names

Ordered Locus Names:

YDR352W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Vacuole membrane; Multi-pass membrane protein Potential Ref.3.
Miscellaneous	Present with 6350 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 2 PQ-loop domains.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Repeat Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	fungal-type vacuole membrane Inferred from direct assay Ref.3. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Vacuolar integral membrane protein YDR352W		PRO_0000253843
Regions
	Transmembrane	14 – 34	21	Helical; Potential
	Transmembrane	40 – 62	23	Helical; Potential
	Transmembrane	72 – 94	23	Helical; Potential
	Transmembrane	144 – 164	21	Helical; Potential
	Transmembrane	185 – 205	21	Helical; Potential
	Transmembrane	216 – 236	21	Helical; Potential
	Transmembrane	250 – 270	21	Helical; Potential
	Domain	8 – 71	64	PQ-loop 1
	Domain	185 – 247	63	PQ-loop 2
Amino acid modifications
	Modified residue	294	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q06328 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F6A41A2175E05C75	FASTA	317	35,179
	10 20 30 40 50 60 MSCSNGIWPT VSNLCGSLSF FTSVISLFPQ IIETYRDKSV DGLSPYFLLA WLCGDITSLI 70 80 90 100 110 120 GAKLTGQLLF QILLAIYFLL NDSFVCGQYY YYGVLHENKL ATVGHEPKPL LPELVENGEL 130 140 150 160 170 180 LREEEDMIQG GSSAESPRSS RRRSAITAAL AIAHTISTAS AYPLNVGSTQ SQVGPPGDGK 190 200 210 220 230 240 NSQLGTILSW IGASFYVGAR IPQLIKNYNR KSTDGLSPFL FATTLLCNIT YNLSIFTSCR 250 260 270 280 290 300 FLDNQNKREF IVNELPFIFG SAGTIAFDLI YFYQYYILYA TDMQLRELER ELYSPEEDSA 310 AQLVTERTSL LSGETQT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28372 Genomic DNA. Translation: AAB64788.1. BK006938 Genomic DNA. Translation: DAA12192.1.
PIR	S61149.
RefSeq	NP_010639.1. NM_001180660.1.
3D structure databases
ProteinModelPortal	Q06328.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q06328. 3 interactions.
MINT	MINT-4482157.
STRING	Q06328.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR352W; YDR352W; YDR352W.
GeneID	851954.
KEGG	sce:YDR352W.
NMPDR	fig|4932.3.peg.1405.
Organism-specific databases
CYGD	YDR352w.
SGD	S000002760. YDR352W.
Phylogenomic databases
eggNOG	fuNOG08072.
GeneTree	EFGT00050000002651.
HOGENOM	HBG396669.
OMA	FLCNDVT.
OrthoDB	EOG4DNJF4.
Gene expression databases
ArrayExpress	Q06328.
Genevestigator	Q06328.
GermOnline	YDR352W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006603. CTNS. [Graphical view]
SMART	SM00679. CTNS. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	970053.

Entry information

Entry name

YD352_YEAST

Accession

Primary (citable) accession number: Q06328
Secondary accession number(s): D6VSY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program