Reviewed, UniProtKB/Swiss-Prot Q06327 (LOX1_ARATH)

Names and origin

Protein names

Recommended name:
    Lipoxygenase 1
    EC=1.13.11.12

Gene names

Name:	LOX1
Ordered Locus Names:	At1g55020
ORF Names:	F14C21.3, F14C21.54

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids, containing a cis,cis-1,4-pentadiene structure.
Catalytic activity	Linoleate + O(2) = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Cofactor	Binds 1 iron ion per subunit. Iron is tightly bound By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Induction	By wounding, abscisic acid (ABA) and methyl jasmonate.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 859	859	Lipoxygenase 1		PRO_0000220703
Regions
	Domain	21 – 161	141	PLAT
	Domain	164 – 859	696	Lipoxygenase
Sites
	Metal binding	519	1	Iron; catalytic By similarity
	Metal binding	524	1	Iron; catalytic By similarity
	Metal binding	711	1	Iron; catalytic By similarity
	Metal binding	715	1	Iron; catalytic By similarity
	Metal binding	859	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06327-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 49378EBACD5FF579	FASTA	859	98,045
	10 20 30 40 50 60 MFGELRDLLT GGGNETTTKK VKGTVVLMKK NVLDFNDFNA SFLDRLHEFL GNKITLRLVS 70 80 90 100 110 120 SDVTDSENGS KGKLGKAAHL EDWITTITSL TAGESAFKVT FDYETDFGYP GAFLIRNSHF 130 140 150 160 170 180 SEFLLKSLTL EDVPGHGRVH YICNSWIYPA KHYTTDRVFF SNKTYLPHET PATLLKYREE 190 200 210 220 230 240 ELVSLRGTGE GELKEWDRVY DYAYYNDLGV PPKNPRPVLG GTQEYPYPRR GRTGRKPTKE 250 260 270 280 290 300 DPQTESRLPI TSSLDIYVPR DERFGHLKMS DFLAYALKAI AQFIQPALEA VFDDTPKEFD 310 320 330 340 350 360 SFEDVLKIYE EGIDLPNQAL IDSIVKNIPL EMLKEIFRTD GQKFLKFPVP QVIKEDKTAW 370 380 390 400 410 420 RTDEEFAREM LAGLNPVVIQ LLKEFPPKSK LDSESYGNQN STITKSHIEH NLDGLTVEEA 430 440 450 460 470 480 LEKERLFILD HHDTLMPYLG RVNTTTTKTY ASRTLLFLKD DGTLKPLVIE LSLPHPNGDK 490 500 510 520 530 540 FGAVSEVYTP GEGVYDSLWQ LAKAFVGVND SGNHQLISHW MQTHASIEPF VIATNRQLSV 550 560 570 580 590 600 LHPVFKLLEP HFRDTMNINA LARQILINGG GIFEITVFPS KYAMEMSSFI YKNHWTFPDQ 610 620 630 640 650 660 ALPAELKKRG MAVEDPEAPH GLRLRIKDYP YAVDGLEVWY AIESWVRDYI FLFYKIEEDI 670 680 690 700 710 720 QTDTELQAWW KEVREEGHGD KKSEPWWPKM QTREELVESC TIIIWVASAL HAAVNFGQYP 730 740 750 760 770 780 VAGYLPNRPT ISRQYMPKEN TPEFEELEKN PDKVFLKTIT AQLQTLLGIS LIEILSTHSS 790 800 810 820 830 840 DEVYLGQRDS KEWAAEKEAL EAFEKFGEKV KEIEKNIDER NDDETLKNRT GLVKMPYTLL 850 FPSSEGGVTG RGIPNSVSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate." Melan M.A., Dong X., Endara M.E., Davis K.R., Ausubel F.M., Peterman T.K. Plant Physiol. 101:441-450(1993) [PubMed: 7506426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Root.
[2]	"Structure and sequence of the Arabidopsis thaliana lipoxygenase 1 gene." Melan M.A., Nemhauser J.M., Peterman T.K. Biochim. Biophys. Acta 1210:377-380(1994) [PubMed: 8305494] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L04637 mRNA. Translation: AAA32827.1. U01843 Genomic DNA. Translation: AAA17036.1. AC069144 Genomic DNA. Translation: AAG51123.1. AY093104 mRNA. Translation: AAM13103.1. BT010358 mRNA. Translation: AAQ56801.1.
PIR	JQ2267.
RefSeq	NP_175900.1.
UniGene	At.19984
3D structure databases
HSSP	HSSP built from PDB template 1FGT based on UniProtKB P08170.
ModBase	Search...
Genome annotation databases
GeneID	841944.
GenomeReviews	Gene locus AT1G55020 in contig CT485782_GR.
KEGG	ath:AT1G55020.
NMPDR	fig|3702.1.peg.5025.
Organism-specific databases
TAIR	At1g55020.
Gene expression databases
ArrayExpress	Q06327.
GermOnline	AT1G55020. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX1_ARATH

Accession

Primary (citable) accession number: Q06327

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 25, 2008
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)