Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Linoleate 9S-lipoxygenase 1

Gene

LOX1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

9S-lipoxygenase that can use linoleic acid or linolenic acid as substrates. Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. Function as regulators of root development by controlling the emergence of lateral roots.2 Publications

Catalytic activityi

Linoleate + O2 = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate.2 Publications

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi519Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi524Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi711Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi715Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi859Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • linoleate 9S-lipoxygenase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • lateral root formation Source: UniProtKB
  • lipid oxidation Source: TAIR
  • oxylipin biosynthetic process Source: UniProtKB-UniPathway
  • response to abscisic acid Source: UniProtKB
  • response to jasmonic acid Source: UniProtKB
  • root development Source: TAIR

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.58 399
ReactomeiR-ATH-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-ATH-2142696 Synthesis of Hepoxilins (HX) and Trioxilins (TrX)
R-ATH-2142700 Synthesis of Lipoxins (LX)
R-ATH-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
R-ATH-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
R-ATH-6798695 Neutrophil degranulation
R-ATH-9018676 Biosynthesis of D-series resolvins
R-ATH-9018677 Biosynthesis of DHA-derived SPMs
R-ATH-9018681 Biosynthesis of protectins
R-ATH-9018682 Biosynthesis of maresins
R-ATH-9018896 Biosynthesis of E-series 18(S)-resolvins
R-ATH-9020265 Biosynthesis of aspirin-triggered D-series resolvins
R-ATH-9023661 Biosynthesis of E-series 18(R)-resolvins
R-ATH-9025106 Biosynthesis of DPAn-6 SPMs
R-ATH-9026286 Biosynthesis of DPAn-3-derived protectins and resolvins
R-ATH-9026290 Biosynthesis of DPAn-3-derived maresins
R-ATH-9026403 Biosynthesis of DPAn-3-derived 13-series resolvins
R-ATH-9027604 Biosynthesis of electrophilic Omega-3 PUFA oxo-derivatives
UniPathwayiUPA00382

Chemistry databases

SwissLipidsiSLP:000001762

Names & Taxonomyi

Protein namesi
Recommended name:
Linoleate 9S-lipoxygenase 1 (EC:1.13.11.58)
Alternative name(s):
Lipoxygenase 1
Short name:
AtLOX1
Gene namesi
Name:LOX1
Ordered Locus Names:At1g55020
ORF Names:F14C21.3, F14C21.54, T24C10.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G55020
TAIRilocus:2011030 AT1G55020

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Increment in the number of lateral roots, and moderate increase in the length of the primary root.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002207031 – 859Linoleate 9S-lipoxygenase 1Add BLAST859

Proteomic databases

PaxDbiQ06327
PRIDEiQ06327

Expressioni

Tissue specificityi

Seedlings, roots, leaves, and flowers (at protein level).4 Publications

Developmental stagei

Transiently expressed during germination, within 1 day after imbibition, especially in the epidermis and the aleurone layer. Later present in the epidermis of the radicle and the adaxial side of the cotyledons. In roots, confined to the pericycle cells and in the lateral root primordia (LRP), and declined at the time of lateral root emergence. Expression is greatly increased in leaves during leaf senescence.3 Publications

Inductioni

By pathogens (e.g. Pseudomonas syringae), wounding, abscisic acid (ABA) and methyl jasmonate (MeJA). Higher levels in light than in dark conditions.2 Publications

Gene expression databases

ExpressionAtlasiQ06327 baseline and differential
GenevisibleiQ06327 AT

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi27169, 1 interactor
STRINGi3702.AT1G55020.1

Structurei

3D structure databases

ProteinModelPortaliQ06327
SMRiQ06327
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 161PLATPROSITE-ProRule annotationAdd BLAST141
Domaini164 – 859LipoxygenasePROSITE-ProRule annotationAdd BLAST696

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Phylogenomic databases

eggNOGiENOG410IH0D Eukaryota
ENOG410YN4N LUCA
HOGENOMiHOG000230469
InParanoidiQ06327
KOiK15718
OMAiLEIWHAI
OrthoDBiEOG093601GX
PhylomeDBiQ06327

Family and domain databases

Gene3Di4.10.372.10, 1 hit
InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001246 LipOase_plant
IPR027433 Lipoxygenase_dom_3
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00468 PLTLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q06327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGELRDLLT GGGNETTTKK VKGTVVLMKK NVLDFNDFNA SFLDRLHEFL
60 70 80 90 100
GNKITLRLVS SDVTDSENGS KGKLGKAAHL EDWITTITSL TAGESAFKVT
110 120 130 140 150
FDYETDFGYP GAFLIRNSHF SEFLLKSLTL EDVPGHGRVH YICNSWIYPA
160 170 180 190 200
KHYTTDRVFF SNKTYLPHET PATLLKYREE ELVSLRGTGE GELKEWDRVY
210 220 230 240 250
DYAYYNDLGV PPKNPRPVLG GTQEYPYPRR GRTGRKPTKE DPQTESRLPI
260 270 280 290 300
TSSLDIYVPR DERFGHLKMS DFLAYALKAI AQFIQPALEA VFDDTPKEFD
310 320 330 340 350
SFEDVLKIYE EGIDLPNQAL IDSIVKNIPL EMLKEIFRTD GQKFLKFPVP
360 370 380 390 400
QVIKEDKTAW RTDEEFAREM LAGLNPVVIQ LLKEFPPKSK LDSESYGNQN
410 420 430 440 450
STITKSHIEH NLDGLTVEEA LEKERLFILD HHDTLMPYLG RVNTTTTKTY
460 470 480 490 500
ASRTLLFLKD DGTLKPLVIE LSLPHPNGDK FGAVSEVYTP GEGVYDSLWQ
510 520 530 540 550
LAKAFVGVND SGNHQLISHW MQTHASIEPF VIATNRQLSV LHPVFKLLEP
560 570 580 590 600
HFRDTMNINA LARQILINGG GIFEITVFPS KYAMEMSSFI YKNHWTFPDQ
610 620 630 640 650
ALPAELKKRG MAVEDPEAPH GLRLRIKDYP YAVDGLEVWY AIESWVRDYI
660 670 680 690 700
FLFYKIEEDI QTDTELQAWW KEVREEGHGD KKSEPWWPKM QTREELVESC
710 720 730 740 750
TIIIWVASAL HAAVNFGQYP VAGYLPNRPT ISRQYMPKEN TPEFEELEKN
760 770 780 790 800
PDKVFLKTIT AQLQTLLGIS LIEILSTHSS DEVYLGQRDS KEWAAEKEAL
810 820 830 840 850
EAFEKFGEKV KEIEKNIDER NDDETLKNRT GLVKMPYTLL FPSSEGGVTG

RGIPNSVSI
Length:859
Mass (Da):98,045
Last modified:June 1, 1994 - v1
Checksum:i49378EBACD5FF579
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04637 mRNA Translation: AAA32827.1
U01843 Genomic DNA Translation: AAA17036.1
AC064840 Genomic DNA Translation: AAG00881.1
AC069144 Genomic DNA Translation: AAG51123.1
CP002684 Genomic DNA Translation: AEE33175.1
AY093104 mRNA Translation: AAM13103.1
BT010358 mRNA Translation: AAQ56801.1
PIRiJQ2267
RefSeqiNP_175900.1, NM_104376.3
UniGeneiAt.19984
At.67309

Genome annotation databases

EnsemblPlantsiAT1G55020.1; AT1G55020.1; AT1G55020
GeneIDi841944
GrameneiAT1G55020.1; AT1G55020.1; AT1G55020
KEGGiath:AT1G55020

Similar proteinsi

Entry informationi

Entry nameiLOX1_ARATH
AccessioniPrimary (citable) accession number: Q06327
Secondary accession number(s): Q9FZ30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health