Reviewed, UniProtKB/Swiss-Prot Q06323 (PSME1_HUMAN)

Names and origin

Protein names

Recommended name:
    Proteasome activator complex subunit 1
Alternative name(s):
    Proteasome activator 28 subunit alpha
      Short name=PA28alpha
      Short name=PA28a
    Activator of multicatalytic protease subunit 1
    11S regulator complex subunit alpha
      Short name=REG-alpha
    Interferon gamma up-regulated I-5111 protein
      Short name=IGUP I-5111

Gene names

Name:	PSME1
Synonyms:	IFI5111

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
Subunit structure	Heterodimer of PSME1 and PSME2, which forms a hexadimeric ring. PSME1 can form homoheptamers.
Induction	By interferon gamma.
Sequence similarities	Belongs to the PA28 family.

Ontologies

Keywords
Cellular component	Proteasome
Coding sequence diversity	Polymorphism
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: HPA proteasome activator complex Inferred from electronic annotation. Source: InterPro
Molecular function	proteasome activator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 249

249

Proteasome activator complex subunit 1

PRO_0000161779

Natural variations

Natural variant

55

1

S → N: dbSNP rs1803830.

VAR_011993

Natural variant

244

1

T → K: dbSNP rs14930.

VAR_011994

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

249

Helix Strand Turn

Details...

Helix

8 – 30

23

Helix

32 – 45

14

Helix

47 – 49

3

Helix

108 – 137

30

Helix

148 – 190

43

Helix

196 – 232

37

Helix

234 – 238

5

Sequences

	Sequence		Length	Mass (Da)
	Q06323-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5E27727E5A0B0AAB	FASTA	249	28,723
	10 20 30 40 50 60 MAMLRVQPEA QAKVDVFRED LCTKTENLLG SYFPKKISEL DAFLKEPALN EANLSNLKAP 70 80 90 100 110 120 LDIPVPDPVK EKEKEERKKQ QEKEDKDEKK KGEDEDKGPP CGPVNCNEKI VVLLQRLKPE 130 140 150 160 170 180 IKDVIEQLNL VTTWLQLQIP RIEDGNNFGV AVQEKVFELM TSLHTKLEGF HTQISKYFSE 190 200 210 220 230 240 RGDAVTKAAK QPHVGDYRQL VHELDEAEYR DIRLMVMEIR NAYAVLYDII LKNFEKLKKP RGETKGMIY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interferon-gamma up-regulates a unique set of proteins in human keratinocytes. Molecular cloning and expression of the cDNA encoding the RGD-sequence-containing protein IGUP I-5111." Honore B., Leffers H., Madsen P., Celis J.E. Eur. J. Biochem. 218:421-430(1993) [PubMed: 8269930] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung fibroblast.
[2]	"Molecular cloning and expression of a gamma-interferon-inducible activator of the multicatalytic protease." Realini C., Dubiel W., Pratt G., Ferrell K., Rechsteiner M. J. Biol. Chem. 269:20727-20732(1994) [PubMed: 8051173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Blood.
[3]	"Organization of the genes encoding the human proteasome activators PA28alpha and beta." McCusker D., Wilson M., Trowsdale J. Immunogenetics 49:438-445(1999) [PubMed: 10199920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[6]	"Characterization of the mouse PA28 activator complex gene family: complete organizations of the three member genes and a physical map of the approximately 150-kb region containing the alpha- and beta-subunit genes." Kohda K., Ishibashi T., Shimbara N., Tanaka K., Matsuda Y., Kasahara M. J. Immunol. 160:4923-4935(1998) [PubMed: 9590240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-118.
[7]	"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes." Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J. Electrophoresis 13:960-969(1992) [PubMed: 1286667] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-35; 191-197 AND 199-209. Tissue: Keratinocyte.
[8]	"The proteasome 11S regulator subunit REG alpha (PA28 alpha) is a heptamer." Johnston S.C., Whitby F.G., Realini C., Rechsteiner M., Hill C.P. Protein Sci. 6:2469-2473(1997) [PubMed: 9385652] [Abstract] Cited for: SUBUNIT.
[9]	"Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], INTERACTION WITH PROTEASOME.
[10]	"Structure of the proteasome activator REGalpha (PA28alpha)." Knowlton J.R., Johnston S.C., Whitby F.G., Realini C., Zhang Z., Rechsteiner M., Hill C.P. Nature 390:639-643(1997) [PubMed: 9403698] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L07633 mRNA. Translation: AAA16521.1.
U10360 Genomic DNA. Translation: AAA53230.1.
AF078829 Genomic DNA. Translation: AAF02217.1.
BT019337 mRNA. Translation: AAV38144.1.
BC000352 mRNA. Translation: AAH00352.1.
BC007503 mRNA. Translation: AAH07503.1.
AB007137 Genomic DNA. Translation: BAA28836.1.

PIR

A54859.

RefSeq

NP_006254.1.
NP_788955.1.

UniGene

Hs.75348

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AVO	X-ray	2.80	A/C/E/G/I/K/M	4-63	[»]
			B/D/F/H/J/L/N	104-242	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q06323.

PTM databases

PhosphoSite

Q06323.

2-D gel databases

SWISS-2DPAGE

Q06323.

Aarhus/Ghent-2DPAGE

5111. IEF.

DOSAC-COBS-2DPAGE

Q06323.

OGP

Q06323.

PHCI-2DPAGE

Q06323.

Genome annotation databases

Ensembl

ENSG00000092010. Homo sapiens. [Contig view]

GeneID

5720.

KEGG

hsa:5720.

Organism-specific databases

H-InvDB

HIX0011554.

HGNC

HGNC:9568. PSME1.

HPA

HPA006632.

MIM

600654. gene.

PharmGKB

PA33914.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOVERGEN

Q06323.

Enzyme and pathway databases

Reactome

REACT_11045. Signaling by Wnt.
REACT_152. Cell Cycle, Mitotic.
REACT_1538. Cell Cycle Checkpoints.
REACT_383. DNA Replication.
REACT_6185. HIV Infection.
REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_9035. APC/C:Cdh1-mediated degradation of Skp2.

Gene expression databases

ArrayExpress

Q06323.

CleanEx

HS_PSME1.

GermOnline

ENSG00000092010. Homo sapiens.

Family and domain databases

InterPro

IPR003185. Proteasome_activ_REG_asu.
IPR009077. Proteasome_activ_REG_asu/bsu.
IPR003186. Proteasome_activ_REG_bsu.
[Graphical view]

Gene3D

G3DSA:1.20.5.120. PA28_alpha-like. 1 hit.
G3DSA:1.20.120.180. PA28_beta. 1 hit.

PANTHER

PTHR10660. Proteasome_activ_REG_asu/bsu. 1 hit.

Pfam

PF02251. PA28_alpha. 1 hit.
PF02252. PA28_beta. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

Q06323.

NextBio

22232.

SOURCE

Search...

Entry information

Entry name

PSME1_HUMAN

Accession

Primary (citable) accession number: Q06323
Secondary accession number(s): Q9UEF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)