Reviewed, UniProtKB/Swiss-Prot Q06321 (ATG26_YEAST)

Names and origin

Protein names

Recommended name:
    Sterol 3-beta-glucosyltransferase
    EC=2.4.1.173
Alternative name(s):
    Autophagy-related protein 26
    UDP-glycosyltransferase 51

Gene names

Name:	ATG26
Synonyms:	UGT51
Ordered Locus Names:	YLR189C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy By similarity. Involved in the biosynthesis of sterol glucoside. Ref.2
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein Ref.4.
Miscellaneous	Present with 396 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Cytoplasm Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ascospore-type prospore membrane assembly Inferred from genetic interaction. Source: SGD autophagy Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: SGD extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sterol 3-beta-glucosyltransferase activity Ref.2 Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DST1	P07273	1	EBI-37737,EBI-19168

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1198	1198	Sterol 3-beta-glucosyltransferase		PRO_0000215616
Regions
	Domain	187 – 236	50	GRAM 1
	Domain	238 – 336	99	PH
	Domain	570 – 636	67	GRAM 2
Amino acid modifications
	Modified residue	128	1	Phosphoserine Ref.6
	Modified residue	131	1	Phosphoserine Ref.6
	Modified residue	476	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q06321-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 74DED87BBBF75B51	FASTA	1,198	136,054
	10 20 30 40 50 60 MPITQIISAS DSEAGPKPSI SLVPDKPSEP ETSPRHHRLS RSLSKFKRWR GRSNSSLSMG 70 80 90 100 110 120 SSEQQELQDS PNEARSDDDE NGYNNDNADD LAKSKYMMKS IAGLLTTASV YAGMNNAQEM 130 140 150 160 170 180 NVLSQVDSEE SDSSDSFQEN IGRNEVKSKK ENLKTKSHPE VPRLDKRKPT LFDFSITREK 190 200 210 220 230 240 LSKDNVAKLR QRFCLDEQEP FLNDFPAWLL KDVLVQGHIF ITTKHFLFFA YLPKNPRSVK 250 260 270 280 290 300 MSGNLNIRTK LIRSTRYWCV LKNHLFSMYT SSTELYFPVL TIDLREVQKI ETQKHTLNGS 310 320 330 340 350 360 ATKTFKLYTD ESTFKFNADS EFSAKSWVNA LKKEQFAAQN SENNSISLKI PLPNIIEIDD 370 380 390 400 410 420 QPIVNKALTL RLRALESSQT YAIDDFMFVF MDGSGSQVKE SLGEQLAILQ KSGVNTLYYD 430 440 450 460 470 480 IPAKKSKSSF GKETPATVEQ KNNGEDSKYL NVPTSAVPSS ENGKKSRFRF RERSNSWFRR 490 500 510 520 530 540 AKPLEDSQVE DVEEIYKDAA NDIDSSVHST IHIHEQEDSQ EQTVAWKPSH LKNFAEMWAA 550 560 570 580 590 600 KPIHYRNKFI PFQKDDTYLI KETEEVSANE RFRYHFKFNK EKSLISTYYT YLNRNVPVYG 610 620 630 640 650 660 KIYVSNDTVC FRSLLPGSNT YMVLPLVDVE TCYKEKGFRF GYFVLVIVIH GHEELFFEFS 670 680 690 700 710 720 TEVARDDIER ILLKLLDNIY ASSAEGSNIS SASLGDVQHN PDSAKLKLFE DKINAEGFEV 730 740 750 760 770 780 PLMIDENPHY KTSIKPNKSY KFGLLTIGSR GDVQPYIALG KGLIKEGHQV VIITHSEFRD 790 800 810 820 830 840 FVESHGIQFE EIAGNPVELM SLMVENESMN VKMLREASSK FRGWIDALLQ TSWEVCNRRK 850 860 870 880 890 900 FDILIESPSA MVGIHITEAL QIPYFRAFTM PWTRTRAYPH AFIVPDQKRG GNYNYLTHVL 910 920 930 940 950 960 FENVFWKGIS GQVNKWRVET LGLGKTNLFL LQQNNVPFLY NVSPTIFPPS IDFSEWVRVT 970 980 990 1000 1010 1020 GYWFLDDKST FKPPAELQEF ISEARSKGKK LVYIGFGSIV VSNAKEMTEA LVEAVMEADV 1030 1040 1050 1060 1070 1080 YCILNKGWSE RLGDKAAKKT EVDLPRNILN IGNVPHDWLF PQVDAAVHHG GSGTTGASLR 1090 1100 1110 1120 1130 1140 AGLPTVIKPF FGDQFFYAGR VEDIGVGIAL KKLNAQTLAD ALKVATTNKI MKDRAGLIKK 1150 1160 1170 1180 1190 KISKEDGIKT AISAIYNELE YARSVTLSRV KTPRKKEENV DATKLTPAET TDEGWTMI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Cloning and functional expression of UGT genes encoding sterol glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideum." Warnecke D.C., Erdmann R., Fahl A., Hube B., Mueller F., Zank T., Zaehringer U., Heinz E. J. Biol. Chem. 274:13048-13059(1999) [PubMed: 10224056] [Abstract] Cited for: FUNCTION.
[3]	"A unified nomenclature for yeast autophagy-related genes." Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y. Dev. Cell 5:539-545(2003) [PubMed: 14536056] [Abstract] Cited for: NOMENCLATURE.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-128; SER-131 AND SER-476, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17246 Genomic DNA. Translation: AAB67475.1.
PIR	S51434.
RefSeq	NP_013290.1.
3D structure databases
SMR	Q06321. Positions 235-342, 741-1155.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6644N.
IntAct	Q06321. 81 interactions.
STRING	Q06321.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Genome annotation databases
Ensembl	YLR189C; YLR189C; YLR189C; Saccharomyces cerevisiae. [Genome view]
GeneID	850886.
KEGG	sce:YLR189C.
NMPDR	fig|4932.3.peg.4300.
Organism-specific databases
CYGD	YLR189c.
SGD	S000004179. ATG26.
Phylogenomic databases
eggNOG	fuNOG07241.
HOGENOM	HBG332791.
OMA	EYPCWLL.
OrthoDB	EOG9908S8.
PhylomeDB	Q06321.
Enzyme and pathway databases
BRENDA	2.4.1.173. 250.
Gene expression databases
ArrayExpress	Q06321.
Genevestigator	Q06321.
GermOnline	YLR189C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002213. UDP_glucos_trans. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 1 hit. PF00169. PH. 1 hit. PF00201. UDPGT. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967248.

Entry information

Entry name

ATG26_YEAST

Accession

Primary (citable) accession number: Q06321

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)