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Protein

Sporulation-specific N-acetylmuramoyl-L-alanine amidase

Gene

cwlC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. CwlC is able to hydrolyze type A cell walls such as B.subtilis. Its main function is to lyze the mother cell wall peptidoglycan, playing a role during sporulation.2 Publications

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zn2+1 Publication

Enzyme regulationi

Inhibited by EDTA.1 Publication

pH dependencei

Optimum pH is 8.5-9.5.1 Publication

Temperature dependencei

Optimum temperature is 60 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10ZincSequence analysis1
Metal bindingi24ZincSequence analysis1
Metal bindingi79ZincSequence analysis1
Active sitei141Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Competence, Sporulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU17410-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation-specific N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
Alternative name(s):
Autolysin
Cell wall hydrolase
Gene namesi
Name:cwlC
Ordered Locus Names:BSU17410
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Secretedcell wall 1 Publication

  • Note: Is associated with the mother cell wall of sporulating cells.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10H → Q: 23% of wild-type activity. 1 Publication1
Mutagenesisi24E → A, Q or S: Loss of activity. 1 Publication1
Mutagenesisi55D → V: Loss of activity. 1 Publication1
Mutagenesisi79H → L: Loss of activity. 1 Publication1
Mutagenesisi79H → Q: 15% of wild-type activity. 1 Publication1
Mutagenesisi81N → I: Loss of activity. 1 Publication1
Mutagenesisi141E → Q or V: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001644182 – 255Sporulation-specific N-acetylmuramoyl-L-alanine amidaseAdd BLAST254

Proteomic databases

PaxDbiQ06320.

Expressioni

Inductioni

Induced at 6 to 7 hours after sporulation.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100009576.

Structurei

Secondary structure

1255
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi185 – 194Combined sources10
Helixi196 – 209Combined sources14
Beta strandi213 – 218Combined sources6
Beta strandi221 – 231Combined sources11
Helixi232 – 245Combined sources14
Beta strandi250 – 254Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X60NMR-A177-255[»]
ProteinModelPortaliQ06320.
SMRiQ06320.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06320.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini180 – 254SPORAdd BLAST75
Repeati184 – 2191Add BLAST36
Repeati220 – 2552Add BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 2552 X 35 AA approximate tandem repeatsAdd BLAST72

Domaini

Contains an N-terminal catalytic domain and two C-terminal tandem repeat sequences that play an important role in peptidoglycan binding.1 Publication

Sequence similaritiesi

Contains 1 SPOR domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105C5C. Bacteria.
COG0860. LUCA.
HOGENOMiHOG000163501.
InParanoidiQ06320.
KOiK01448.
OMAiLQDANPG.
PhylomeDBiQ06320.

Family and domain databases

CDDicd02696. MurNAc-LAA. 1 hit.
Gene3Di3.30.70.1070. 1 hit.
3.40.630.40. 1 hit.
InterProiIPR002508. MurNAc-LAA_cat.
IPR007730. SPOR_dom.
[Graphical view]
PfamiPF01520. Amidase_3. 1 hit.
PF05036. SPOR. 1 hit.
[Graphical view]
SMARTiSM00646. Ami_3. 1 hit.
[Graphical view]
SUPFAMiSSF110997. SSF110997. 1 hit.
PROSITEiPS51724. SPOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06320-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKIFIDPGH GGSDPGATGN GLQEKTLTLQ IALALRTILT NEYEGVSLLL
60 70 80 90 100
SRTSDQYVSL NDRTNAANNW GADFFLSIHV NSGGGTGFES YIYPDVGAPT
110 120 130 140 150
TTYQSTIHSE VIQAVDFADR GKKTANFHVL RESAMPALLT ENGFIDTVSD
160 170 180 190 200
ANKLKTSSFI QSLARGHANG LEQAFNLKKT SSSGLYKVQI GAFKVKANAD
210 220 230 240 250
SLASNAEAKG FDSIVLLKDG LYKVQIGAFS SKDNADTLAA RAKNAGFDAI

VILES
Length:255
Mass (Da):27,146
Last modified:February 1, 1995 - v1
Checksum:i30C950116784FBC1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti158S → T in CAA92813 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14666 Genomic DNA. Translation: BAA03500.1.
AL009126 Genomic DNA. Translation: CAB13625.2.
Z68500 Genomic DNA. Translation: CAA92813.1.
PIRiA36935.
RefSeqiNP_389623.2. NC_000964.3.
WP_003244862.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13625; CAB13625; BSU17410.
GeneIDi940088.
KEGGibsu:BSU17410.
PATRICi18975293. VBIBacSub10457_1838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14666 Genomic DNA. Translation: BAA03500.1.
AL009126 Genomic DNA. Translation: CAB13625.2.
Z68500 Genomic DNA. Translation: CAA92813.1.
PIRiA36935.
RefSeqiNP_389623.2. NC_000964.3.
WP_003244862.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X60NMR-A177-255[»]
ProteinModelPortaliQ06320.
SMRiQ06320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100009576.

Proteomic databases

PaxDbiQ06320.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13625; CAB13625; BSU17410.
GeneIDi940088.
KEGGibsu:BSU17410.
PATRICi18975293. VBIBacSub10457_1838.

Phylogenomic databases

eggNOGiENOG4105C5C. Bacteria.
COG0860. LUCA.
HOGENOMiHOG000163501.
InParanoidiQ06320.
KOiK01448.
OMAiLQDANPG.
PhylomeDBiQ06320.

Enzyme and pathway databases

BioCyciBSUB:BSU17410-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ06320.

Family and domain databases

CDDicd02696. MurNAc-LAA. 1 hit.
Gene3Di3.30.70.1070. 1 hit.
3.40.630.40. 1 hit.
InterProiIPR002508. MurNAc-LAA_cat.
IPR007730. SPOR_dom.
[Graphical view]
PfamiPF01520. Amidase_3. 1 hit.
PF05036. SPOR. 1 hit.
[Graphical view]
SMARTiSM00646. Ami_3. 1 hit.
[Graphical view]
SUPFAMiSSF110997. SSF110997. 1 hit.
PROSITEiPS51724. SPOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCWLC_BACSU
AccessioniPrimary (citable) accession number: Q06320
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.