Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sporulation-specific N-acetylmuramoyl-L-alanine amidase

Gene

cwlC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. CwlC is able to hydrolyze type A cell walls such as B.subtilis. Its main function is to lyze the mother cell wall peptidoglycan, playing a role during sporulation.2 Publications

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zn2+1 Publication

Enzyme regulationi

Inhibited by EDTA.1 Publication

pH dependencei

Optimum pH is 8.5-9.5.1 Publication

Temperature dependencei

Optimum temperature is 60 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10ZincSequence analysis1
Metal bindingi24ZincSequence analysis1
Metal bindingi79ZincSequence analysis1
Active sitei141Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCell wall biogenesis/degradation, Competence, Sporulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU17410-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation-specific N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
Alternative name(s):
Autolysin
Cell wall hydrolase
Gene namesi
Name:cwlC
Ordered Locus Names:BSU17410
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • cell wall 1 Publication
  • Note: Is associated with the mother cell wall of sporulating cells.

GO - Cellular componenti

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10H → Q: 23% of wild-type activity. 1 Publication1
Mutagenesisi24E → A, Q or S: Loss of activity. 1 Publication1
Mutagenesisi55D → V: Loss of activity. 1 Publication1
Mutagenesisi79H → L: Loss of activity. 1 Publication1
Mutagenesisi79H → Q: 15% of wild-type activity. 1 Publication1
Mutagenesisi81N → I: Loss of activity. 1 Publication1
Mutagenesisi141E → Q or V: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001644182 – 255Sporulation-specific N-acetylmuramoyl-L-alanine amidaseAdd BLAST254

Proteomic databases

PaxDbiQ06320

Expressioni

Inductioni

Induced at 6 to 7 hours after sporulation.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100009576

Structurei

Secondary structure

1255
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi185 – 194Combined sources10
Helixi196 – 209Combined sources14
Beta strandi213 – 218Combined sources6
Beta strandi221 – 231Combined sources11
Helixi232 – 245Combined sources14
Beta strandi250 – 254Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X60NMR-A177-255[»]
ProteinModelPortaliQ06320
SMRiQ06320
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06320

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini180 – 254SPORAdd BLAST75
Repeati184 – 2191Add BLAST36
Repeati220 – 2552Add BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 2552 X 35 AA approximate tandem repeatsAdd BLAST72

Domaini

Contains an N-terminal catalytic domain and two C-terminal tandem repeat sequences that play an important role in peptidoglycan binding.1 Publication

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105C5C Bacteria
COG0860 LUCA
HOGENOMiHOG000163501
InParanoidiQ06320
KOiK01448
OMAiFKVKANA
PhylomeDBiQ06320

Family and domain databases

CDDicd02696 MurNAc-LAA, 1 hit
Gene3Di3.30.70.1070, 1 hit
InterProiView protein in InterPro
IPR002508 MurNAc-LAA_cat
IPR007730 SPOR-like_dom
IPR036680 SPOR-like_sf
PfamiView protein in Pfam
PF01520 Amidase_3, 1 hit
PF05036 SPOR, 1 hit
SMARTiView protein in SMART
SM00646 Ami_3, 1 hit
SUPFAMiSSF110997 SSF110997, 1 hit
PROSITEiView protein in PROSITE
PS51724 SPOR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06320-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKIFIDPGH GGSDPGATGN GLQEKTLTLQ IALALRTILT NEYEGVSLLL
60 70 80 90 100
SRTSDQYVSL NDRTNAANNW GADFFLSIHV NSGGGTGFES YIYPDVGAPT
110 120 130 140 150
TTYQSTIHSE VIQAVDFADR GKKTANFHVL RESAMPALLT ENGFIDTVSD
160 170 180 190 200
ANKLKTSSFI QSLARGHANG LEQAFNLKKT SSSGLYKVQI GAFKVKANAD
210 220 230 240 250
SLASNAEAKG FDSIVLLKDG LYKVQIGAFS SKDNADTLAA RAKNAGFDAI

VILES
Length:255
Mass (Da):27,146
Last modified:February 1, 1995 - v1
Checksum:i30C950116784FBC1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti158S → T in CAA92813 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14666 Genomic DNA Translation: BAA03500.1
AL009126 Genomic DNA Translation: CAB13625.2
Z68500 Genomic DNA Translation: CAA92813.1
PIRiA36935
RefSeqiNP_389623.2, NC_000964.3
WP_003244862.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13625; CAB13625; BSU17410
GeneIDi940088
KEGGibsu:BSU17410
PATRICifig|224308.179.peg.1887

Similar proteinsi

Entry informationi

Entry nameiCWLC_BACSU
AccessioniPrimary (citable) accession number: Q06320
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health