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Q06319 (ACDS_MEGEL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acyl-CoA dehydrogenase, short-chain specific

EC=1.3.8.1
Alternative name(s):
Butyryl-CoA dehydrogenase
Short name=BCAD
SCAD
OrganismMegasphaera elsdenii
Taxonomic identifier907 [NCBI]
Taxonomic lineageBacteriaFirmicutesNegativicutesSelenomonadalesVeillonellaceaeMegasphaera

Protein attributes

Sequence length383 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has an optimum specificity for 4-carbon length fatty acyl-CoAs.

Catalytic activity

Butanoyl-CoA + electron-transfer flavoprotein = 2-butenoyl-CoA + reduced electron-transfer flavoprotein.

Cofactor

FAD.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the acyl-CoA dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 383383Acyl-CoA dehydrogenase, short-chain specific
PRO_0000201190

Sites

Active site3671Proton acceptor

Experimental info

Mutagenesis3671E → Q: Loss of activity.

Secondary structure

.............................................................. 383
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q06319 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 3D68AAE34D9BBAB8

FASTA38341,408
        10         20         30         40         50         60 
MDFNLTDIQQ DFLKLAHDFG EKKLAPTVTE RDHKGIYDKE LIDELLSLGI TGAYFEEKYG 

        70         80         90        100        110        120 
GSGDDGGDVL SYILAVEELA KYDAGVAITL SATVSLCANP IWQFGTEAQK EKFLVPLVEG 

       130        140        150        160        170        180 
TKLGAFGLTE PNAGTDASGQ QTIATKNDDG TYTLNGSKIF ITNGGAADIY IVFAMTDKSK 

       190        200        210        220        230        240 
GNHGITAFIL EDGTPGFTYG KKEDKMGIHT SQTMELVFQD VKVPAENMLG EEGKGFKIAM 

       250        260        270        280        290        300 
MTLDGGRIGV AAQALGIAEA ALADAVEYSK QRVQFGKPLC KFQSISFKLA DMKMQIEAAR 

       310        320        330        340        350        360 
NLVYKAACKK QEGKPFTVDA AIAKRVASDV AMRVTTEAVQ IFGGYGYSEE YPVARHMRDA 

       370        380 
KITQIYEGTN EVQLMVTGGA LLR 

« Hide

References

[1]"Characterization of wild-type and an active-site mutant in Escherichia coli of short-chain acyl-CoA dehydrogenase from Megasphaera elsdenii."
Becker D.F., Fuchs J.A., Banfield D.K., Funk W.D., Macgillivray R.T.A., Stankovich M.T.
Biochemistry 32:10736-10742(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-50.
[2]"Three-dimensional structure of butyryl-CoA dehydrogenase from Megasphaera elsdenii."
Djordjevic S., Pace C.P., Stankovich M.T., Kim J.-J.P.
Biochemistry 34:2163-2171(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L04528 Unassigned DNA. Translation: AAA03594.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BUCX-ray2.50A/B1-383[»]
ProteinModelPortalQ06319.
SMRQ06319. Positions 1-383.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-11937.

Family and domain databases

Gene3D1.10.540.10. 1 hit.
2.40.110.10. 1 hit.
InterProIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEPS00072. ACYL_COA_DH_1. 1 hit.
PS00073. ACYL_COA_DH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ06319.

Entry information

Entry nameACDS_MEGEL
AccessionPrimary (citable) accession number: Q06319
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 16, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references