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Protein

Beta-lactamase

Gene

bla

Organism
Amycolatopsis lactamdurans (Nocardia lactamdurans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Active on penicillins but not on cephalosporins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Acyl-ester intermediateBy similarity
Sitei88 – 881Increases nucleophilicity of active site SerBy similarity
Binding sitei143 – 1431SubstrateBy similarity
Active sitei179 – 1791Proton acceptorBy similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:bla
OrganismiAmycolatopsis lactamdurans (Nocardia lactamdurans)
Taxonomic identifieri1913 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeAmycolatopsis

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Chaini30 – 302273Beta-lactamasePRO_0000017006Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ06316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni247 – 2493Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06316-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRRRVHAW ARARPAAPEP APPTPSAAAP SVAPGPAATP PDPAVEQEFT
60 70 80 90 100
RLQTQYDARL GLYAVDTGSG ESVAFRADER FAFASTFKAL AAAAVLDSTT
110 120 130 140 150
PQQLDQVVRY SKDELLENSP ITKDHVATGM TLRELCDAAV RFSDNTAGNL
160 170 180 190 200
LLKHVGGPQG LDAALTAVGD EVTSADRWEP ELNSAVPGDV RDTSTPRALA
210 220 230 240 250
HDLRQFVLGD ALAEDDRALL TDWLRRNTTG GTVIRAGVPA DWVVGDKTGS
260 270 280 290 300
GYYGGRNDIA VLWPPNRAPI VMAVMTSREE PRAKRADALL ADAARVAVTA

LG
Length:302
Mass (Da):32,084
Last modified:June 1, 1994 - v1
Checksum:iB7D6550AAE88B4F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z13971 Genomic DNA. Translation: CAA78373.1.
PIRiS36188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z13971 Genomic DNA. Translation: CAA78373.1.
PIRiS36188.

3D structure databases

ProteinModelPortaliQ06316.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genes for a beta-lactamase, a penicillin-binding protein and a transmembrane protein are clustered with the cephamycin biosynthetic genes in Nocardia lactamdurans."
    Coque J.J.R., Liras P., Martin J.F.
    EMBO J. 12:631-639(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LC411.

Entry informationi

Entry nameiBLAC_AMYLA
AccessioniPrimary (citable) accession number: Q06316
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: January 7, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.