Q06316 (BLAC_NOCLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase
EC=3.5.2.6

Alternative name(s):
Penicillinase

Gene names

Name:

bla

Organism

Nocardia lactamdurans

Taxonomic identifier

1913 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Active on penicillins but not on cephalosporins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Secreted.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 302	273	Beta-lactamase		PRO_0000017006
Regions
	Region	247 – 249	3	Substrate binding By similarity
Sites
	Active site	85	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06316 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: B7D6550AAE88B4F4	FASTA	302	32,084
	10 20 30 40 50 60 MADRRRVHAW ARARPAAPEP APPTPSAAAP SVAPGPAATP PDPAVEQEFT RLQTQYDARL 70 80 90 100 110 120 GLYAVDTGSG ESVAFRADER FAFASTFKAL AAAAVLDSTT PQQLDQVVRY SKDELLENSP 130 140 150 160 170 180 ITKDHVATGM TLRELCDAAV RFSDNTAGNL LLKHVGGPQG LDAALTAVGD EVTSADRWEP 190 200 210 220 230 240 ELNSAVPGDV RDTSTPRALA HDLRQFVLGD ALAEDDRALL TDWLRRNTTG GTVIRAGVPA 250 260 270 280 290 300 DWVVGDKTGS GYYGGRNDIA VLWPPNRAPI VMAVMTSREE PRAKRADALL ADAARVAVTA LG

References

[1]

"Genes for a beta-lactamase, a penicillin-binding protein and a transmembrane protein are clustered with the cephamycin biosynthetic genes in Nocardia lactamdurans." Coque J.J.R., Liras P., Martin J.F. EMBO J. 12:631-639(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LC411.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z13971 Genomic DNA. Translation: CAA78373.1.
PIR	S36188.
3D structure databases
ProteinModelPortal	Q06316.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_NOCLA

Accession

Primary (citable) accession number: Q06316

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program