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Protein

Protein SKG3

Gene

SKG3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in cell wall integrity.1 Publication

Enzyme and pathway databases

BioCyciYEAST:G3O-32310-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SKG3
Alternative name(s):
Suppressor of lethality of KEX2-GAS1 double null mutant protein 3
Gene namesi
Name:SKG3
Ordered Locus Names:YLR187W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR187W.
SGDiS000004177. SKG3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10261026Protein SKG3PRO_0000269649Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101PhosphoserineCombined sources
Modified residuei31 – 311PhosphoserineCombined sources
Modified residuei578 – 5781PhosphothreonineCombined sources
Modified residuei580 – 5801PhosphoserineCombined sources
Modified residuei592 – 5921PhosphoserineCombined sources
Modified residuei701 – 7011PhosphoserineCombined sources
Modified residuei954 – 9541PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06315.

PTM databases

iPTMnetiQ06315.

Interactioni

Subunit structurei

Interacts with phosphatidylinositol 3-phosphate.1 Publication

Protein-protein interaction databases

BioGridi31457. 23 interactions.
DIPiDIP-6525N.
IntActiQ06315. 3 interactions.
MINTiMINT-705771.

Structurei

3D structure databases

ProteinModelPortaliQ06315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini90 – 221132PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini254 – 433180PH 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00530000068670.
HOGENOMiHOG000057106.
InParanoidiQ06315.
KOiK12609.
OrthoDBiEOG7HF1V1.

Family and domain databases

InterProiIPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIFSGVKS PKLSAPPKVF KNDESPSTPS SPKFDQGLRS LSASASRLFS
60 70 80 90 100
NSISTPGSPT LDLPQEHSIN GDISPELVPI VTLLSAQAHR RYHYGIFLIL
110 120 130 140 150
HDLKTDGTPA ARQWEECYGV LLGTQLALWD AKELSDSKNN KNTSTMKKAA
160 170 180 190 200
SRPSFINFTD ASVRSLDAND QVIIASENEK TKKDLDNVLV VSTTLKNRYF
210 220 230 240 250
LKFKNSKSFK TWNAAIRLSL FEFTALQEAY TGSFLSSRGV KLGDIKVVMA
260 270 280 290 300
DTKFTYEDWV SVRFGTGMPW KRCYAVISPQ SGKKKKNSKG SICFYENNKK
310 320 330 340 350
TKKSNIMTTV VDARALYAVY PSSPILIDTS TIIKLEGFVS FDKSEEPQET
360 370 380 390 400
NLFIMPEKHQ GVPGYDTIIR FLIPAMNAFY LYGRPKGLIA NRTDPDSLLF
410 420 430 440 450
ALPTLPHIYY LQVDDVLSLT KDKNYIHWSA ADWRNNIVQV LQKKLSKGYK
460 470 480 490 500
GCGNKTVSVS SGMMKSPAIS SAELFEGYDS LPERQMESPQ KSKMKSPTLA
510 520 530 540 550
STDDINSASA SVNSHATSVK QTELFVTDNS SKINDSVSAQ SSVTTNFKDT
560 570 580 590 600
FTTPMTSGML NHENSERSFG SGLKLKITDS NLENMEDVEA KSANEFSTTP
610 620 630 640 650
EDKHIHLANA AELSALYDKY STSPFGKSEA NSSPKPQKLE VKDRSKNENR
660 670 680 690 700
SPYERYVGTS AESKTFEIGN VRESKSTINT SLSSPLRVED SRRSKNEDLG
710 720 730 740 750
SLKEFEELSQ KISNMGMANI SSEALSDTAE NSSFVTDLNL NINNSSSVNL
760 770 780 790 800
NEEQRVPDFG EENVFDPDYM EQNQMLETES RYTTDEFDFS DNQDAASSNY
810 820 830 840 850
SNGQTNRTVT ETLSASDRND KIPHSSLFTN LNQLTSNGGN YQDREDFSGD
860 870 880 890 900
QINKPQQSQP LHVKGPQTSS FGYRNSSANS SQPQAPYPVG RPLGKIRTGP
910 920 930 940 950
LTVQPMQQGG NSSMYSFQSS QHRFHSSQQR QNQSLSFRNN TYGSGNNQNT
960 970 980 990 1000
FHPSPQLQQQ PQNMRYLNNK LPINDRSPIP QTQHHVPDGR PSLHINTTNR
1010 1020
TNPLTAQSGF SQFMPPNSTS TNPYSS
Length:1,026
Mass (Da):114,111
Last modified:November 1, 1996 - v1
Checksum:i19505E5E694FC47A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17246 Genomic DNA. Translation: AAB67456.1.
BK006945 Genomic DNA. Translation: DAA09506.1.
PIRiS51432.
RefSeqiNP_013288.1. NM_001182074.1.

Genome annotation databases

EnsemblFungiiYLR187W; YLR187W; YLR187W.
GeneIDi850884.
KEGGisce:YLR187W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17246 Genomic DNA. Translation: AAB67456.1.
BK006945 Genomic DNA. Translation: DAA09506.1.
PIRiS51432.
RefSeqiNP_013288.1. NM_001182074.1.

3D structure databases

ProteinModelPortaliQ06315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31457. 23 interactions.
DIPiDIP-6525N.
IntActiQ06315. 3 interactions.
MINTiMINT-705771.

PTM databases

iPTMnetiQ06315.

Proteomic databases

MaxQBiQ06315.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR187W; YLR187W; YLR187W.
GeneIDi850884.
KEGGisce:YLR187W.

Organism-specific databases

EuPathDBiFungiDB:YLR187W.
SGDiS000004177. SKG3.

Phylogenomic databases

GeneTreeiENSGT00530000068670.
HOGENOMiHOG000057106.
InParanoidiQ06315.
KOiK12609.
OrthoDBiEOG7HF1V1.

Enzyme and pathway databases

BioCyciYEAST:G3O-32310-MONOMER.

Miscellaneous databases

PROiQ06315.

Family and domain databases

InterProiIPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. "Genome-wide analysis of membrane targeting by S.cerevisiae pleckstrin homology domains."
    Yu J.W., Mendrola J.M., Audhya A., Singh S., Keleti D., DeWald D.B., Murray D., Emr S.D., Lemmon M.A.
    Mol. Cell 13:677-688(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN, INTERACTION WITH PHOSPHATIDYLINOSITOL-3-PHOSPHATE.
  5. "SKG1, a suppressor gene of synthetic lethality of kex2Deltagas1Delta mutations, encodes a novel membrane protein that affects cell wall composition."
    Tomishige N., Noda Y., Adachi H., Shimoi H., Yoda K.
    Yeast 22:141-155(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10 AND SER-701, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-578; SER-592 AND SER-701, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31; THR-578; SER-580; SER-592; SER-701 AND SER-954, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSKG3_YEAST
AccessioniPrimary (citable) accession number: Q06315
Secondary accession number(s): D6VYJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.