Reviewed, UniProtKB/Swiss-Prot Q06283 (LYSC2_BOVIN)

Names and origin

Protein names

Recommended name:
    Lysozyme C-2
    EC=3.2.1.17
Alternative name(s):
    1,4-beta-N-acetylmuramidase C

Gene names

Name:

LYZ2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer.
Tissue specificity	Stomach-specific.
Miscellaneous	Lysozyme C is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides. The ruminant gastric lysozymes, which digest symbiotic bacteria coming with cud from the rumen, are much more resistant to inactivation by pepsin than are other lysozymes.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.

Ontologies

Keywords
Biological process	Digestion
Domain	Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW digestion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

By similarity

Chain

19 – 147

129

Lysozyme C-2

PRO_0000018455

Sites

Active site

53

1

By similarity

Active site

71

1

By similarity

Amino acid modifications

Disulfide bond

24 ↔ 145

By similarity

Disulfide bond

48 ↔ 133

By similarity

Disulfide bond

83 ↔ 99

By similarity

Disulfide bond

95 ↔ 113

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

147

Helix Strand Turn

Details...

Helix

23 – 32

10

Helix

43 – 54

12

Beta strand

61 – 64

4

Turn

65 – 68

4

Beta strand

69 – 72

4

Turn

73 – 76

4

Turn

79 – 81

3

Beta strand

82 – 84

3

Beta strand

88 – 90

3

Beta strand

96 – 98

3

Helix

99 – 103

5

Beta strand

104 – 106

3

Helix

108 – 121

14

Helix

123 – 125

3

Helix

127 – 132

6

Turn

133 – 135

3

Helix

139 – 142

4

Sequences

	Sequence		Length	Mass (Da)
	Q06283-1 [UniParc]. Last modified January 1, 1999. Version 2. Checksum: B0A38B9ECE1E66BE	FASTA	147	16,304
	10 20 30 40 50 60 MKALVILGFL FLSVAVQGKV FERCELARTL KKLGLDGYKG VSLANWLCLT KWESSYNTKA 70 80 90 100 110 120 TNYNPSSEST DYGIFQINSK WWCNDGKTPN AVDGCHVSCS ELMENDIAKA VACAKHIVSE 130 140 QGITAWVAWK SHCRDHDVSS YVEGCTL

References

[1]	"Characterization of the cow stomach lysozyme genes: repetitive DNA and concerted evolution." Irwin D.M., White R.T., Wilson A.C. J. Mol. Evol. 37:355-366(1993) [PubMed: 8308905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M95098 Unassigned DNA. Translation: AAC37311.1.

IPI

IPI00718701.

PIR

LZBO. C34277.

RefSeq

NP_851342.1.

UniGene

Bt.29367

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2Z2F	X-ray	1.50	A	19-147	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH22. Glycoside Hydrolase Family 22.

Genome annotation databases

Ensembl

ENSBTAG00000026088. Bos taurus. [Contig view]

GeneID

280849.

KEGG

bta:280849.

Phylogenomic databases

HOVERGEN

Q06283.

Enzyme and pathway databases

BRENDA

3.2.1.17. 251.

Family and domain databases

InterPro

IPR001916. Glyco_hydro_22.
IPR019799. Glyco_hydro_22_CS.
IPR000974. Glyco_hydro_22_lys.
[Graphical view]

Pfam

PF00062. Lys. 1 hit.
[Graphical view]

PRINTS

PR00137. LYSOZYME.
PR00135. LYZLACT.

SMART

SM00263. LYZ1. 1 hit.
[Graphical view]

PROSITE

PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit.
PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LYSC2_BOVIN

Accession

Primary (citable) accession number: Q06283

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	January 1, 1999
Last modified:	June 16, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)