Reviewed, UniProtKB/Swiss-Prot Q06245 (SEC10_YEAST)

Names and origin

Protein names

Recommended name:
    Exocyst complex component SEC10

Gene names

Name:	SEC10
Ordered Locus Names:	YLR166C
ORF Names:	L9362.12

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	871 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the exocyst complex involved in the docking of exocystic vesicles with fusion sites on the plasma membrane.
Subunit structure	The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84.
Miscellaneous	Present with 2340 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the SEC10 family.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	Golgi to plasma membrane transport Traceable author statement. Source: SGD bipolar cellular bud site selection Traceable author statement. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle docking during exocytosis Traceable author statement. Source: SGD vesicle fusion Traceable author statement. Source: SGD
Cellular component	exocyst Ref.1 Inferred from physical interaction. Source: SGD mating projection tip Inferred from direct assay. Source: SGD
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EXO84	P38261	2	EBI-16504,EBI-21567
SEC15	P22224	1	EBI-16504,EBI-16543

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 871	871	Exocyst complex component SEC10		PRO_0000118950
Regions
	Coiled coil	74 – 101	28	Potential
Amino acid modifications
	Modified residue	142	1	Phosphoserine Ref.4 Ref.5
	Modified residue	485	1	Phosphoserine Ref.5 Ref.6
	Modified residue	507	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q06245-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A32073099815388D	FASTA	871	100,342
	10 20 30 40 50 60 MNSLYELDPK WKKLLKTDNF LGGLTVNEFV QELSKDHRND VLIDANTKNL PTNEKDQDAI 70 80 90 100 110 120 REAIWKQLDP KPYIRTFEST LKELKNLNEE TLNKRQYFSE QVATQEVIHS ENVIKLSKDL 130 140 150 160 170 180 HTTLLTFDKL DDRLTNVTQV VSPLGDKLET AIKKKQNYIQ SVELIRRYND FYSMGKSDIV 190 200 210 220 230 240 EQLRLSKNWK LNLKSVKLMK NLLILSSKLE TSSIPKTINT KLVIEKYSEM MENELLENFN 250 260 270 280 290 300 SAYRENNFTK LNEIAIILNN FNGGVNVIQS FINQHDYFID TKQIDLENEF ENVFIKNVKF 310 320 330 340 350 360 KEQLIDFENH SVIIETSMQN LINDVETVIK NESKIVKRVF EEKATHVIQL FIQRVFAQKI 370 380 390 400 410 420 EPRFEVLLRN SLSISNLAYV RILHGLFTLF GKFTKSLIDY FQLLEIDDSN QILSTTLEQC 430 440 450 460 470 480 FADLFSHYLY DRSKYFGIEK RSLEAILVDM TSKFTVNYDK EINKRVLLDK YKEKLSTNVD 490 500 510 520 530 540 AFMHSPRGNT HSRQDSTSRS KLSQFNSFLK THLDKDHLSL NRTNTLSDSF NNSSSSTQYD 550 560 570 580 590 600 VANNSSSLVN SSFTASDIDN SPNSPANYSL NDVDSMLKCV VESTARVMEL IPNKAHLYIL 610 620 630 640 650 660 EILKIMFLGI VDSYMEIALE VAYWKICKVD INKTAGVVNL NFLKFISMST EILDLLSISI 670 680 690 700 710 720 KSIFLPLLNN SPEIKAQIIE MTNSQIQKME ILINIILQET ITVISTKFSA ILCKQKKKDF 730 740 750 760 770 780 VPKSQELLDQ DTLPAIEIVN ILNLIFEQSS KFLKGKNLQT FLTLIGEELY GLLLSHYSHF 790 800 810 820 830 840 QVNSIGGVVV TKDIIGYQTA IEDWGVASLI DKFATLRELA NLFTVQPELL ESLTKEGHLA 850 860 870 DIGRDIIQSY ISNREDFNHD NFINSVKLNF R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae." TerBush D.R., Maurice T., Roth D., Novick P. EMBO J. 15:6483-6494(1996) [PubMed: 8978675] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 347-353 AND 856-867.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, MASS SPECTROMETRY.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142 AND SER-485, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-485 AND SER-507, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y08789 Genomic DNA. Translation: CAA70041.1. U51921 Genomic DNA. Translation: AAB67490.1. U17246 Genomic DNA. Translation: AAB67476.2.
PIR	S68482.
RefSeq	NP_013267.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1724N.
IntAct	Q06245. 17 interactions.
STRING	Q06245.
Genome annotation databases
Ensembl	YLR166C; YLR166C; YLR166C; Saccharomyces cerevisiae. [Genome view]
GeneID	850863.
KEGG	sce:YLR166C.
NMPDR	fig|4932.3.peg.4277.
Organism-specific databases
CYGD	YLR166c.
SGD	S000004156. SEC10.
Phylogenomic databases
HOGENOM	Q06245.
OMA	DPKPYIR
OrthoDB	EOG9RBS1V
Gene expression databases
ArrayExpress	Q06245.
Genevestigator	Q06245.
GermOnline	YLR166C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009976. Sec10. [Graphical view]
PANTHER	PTHR12100. Sec10. 1 hit.
Pfam	PF07393. Sec10. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967188.

Entry information

Entry name

SEC10_YEAST

Accession

Primary (citable) accession number: Q06245
Secondary accession number(s): P87329

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)