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Protein

Endoribonuclease YSH1

Gene

YSH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Has endonuclease activity.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi68Zinc 1By similarity1
Metal bindingi70Zinc 1By similarity1
Metal bindingi72Zinc 2By similarity1
Metal bindingi73Zinc 2By similarity1
Metal bindingi163Zinc 1By similarity1
Metal bindingi184Zinc 1By similarity1
Metal bindingi184Zinc 2By similarity1
Active sitei408Proton donorSequence analysis1
Metal bindingi430Zinc 2By similarity1

GO - Molecular functioni

  • endoribonuclease activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • mRNA polyadenylation Source: SGD
  • pre-mRNA cleavage required for polyadenylation Source: SGD
  • RNA splicing Source: SGD
  • snoRNA 3'-end processing Source: SGD
  • snoRNA splicing Source: SGD
  • termination of RNA polymerase II transcription Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32376-MONOMER.
ReactomeiR-SCE-77595. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease YSH1 (EC:3.1.27.-)
Alternative name(s):
Yeast 73 kDa homolog 1
mRNA 3'-end-processing protein YSH1
Gene namesi
Name:YSH1
Synonyms:BRR5
Ordered Locus Names:YLR277C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR277C.
SGDiS000004267. YSH1.

Subcellular locationi

GO - Cellular componenti

  • mRNA cleavage and polyadenylation specificity factor complex Source: SGD
  • mRNA cleavage factor complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi37D → N: Loss of endonuclease activity. 1 Publication1
Mutagenesisi163H → F: Loss of endonuclease activity. 1 Publication1
Mutagenesisi184D → N: Loss of endonuclease activity. 1 Publication1
Mutagenesisi209E → Q: Loss of endonuclease activity. 1 Publication1
Mutagenesisi408H → F: Loss of endonuclease activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000763681 – 779Endoribonuclease YSH1Add BLAST779

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei517Phosphoserine; by ATM or ATRCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06224.
PRIDEiQ06224.

PTM databases

iPTMnetiQ06224.

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of at least PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Interacts with FIP1, PFS2, RNA14 and YTH1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
YTH1Q061026EBI-38345,EBI-38049

Protein-protein interaction databases

BioGridi31545. 32 interactors.
DIPiDIP-2470N.
IntActiQ06224. 23 interactors.
MINTiMINT-375457.

Structurei

3D structure databases

ProteinModelPortaliQ06224.
SMRiQ06224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00860000133746.
HOGENOMiHOG000203394.
InParanoidiQ06224.
KOiK14403.
OMAiVLIPRRC.
OrthoDBiEOG092C11WH.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR022712. Beta_Casp.
IPR021718. CPSF73-100_C.
IPR001279. Metallo-B-lactamas.
IPR011108. RMMBL.
[Graphical view]
PfamiPF10996. Beta-Casp. 1 hit.
PF11718. CPSF73-100_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
PF07521. RMMBL. 1 hit.
[Graphical view]
SMARTiSM01027. Beta-Casp. 1 hit.
SM01098. CPSF73-100_C. 1 hit.
SM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERTNTTTFK FFSLGGSNEV GRSCHILQYK GKTVMLDAGI HPAYQGLASL
60 70 80 90 100
PFYDEFDLSK VDILLISHFH LDHAASLPYV MQRTNFQGRV FMTHPTKAIY
110 120 130 140 150
RWLLRDFVRV TSIGSSSSSM GTKDEGLFSD EDLVDSFDKI ETVDYHSTVD
160 170 180 190 200
VNGIKFTAFH AGHVLGAAMF QIEIAGLRVL FTGDYSREVD RHLNSAEVPP
210 220 230 240 250
LSSNVLIVES TFGTATHEPR LNRERKLTQL IHSTVMRGGR VLLPVFALGR
260 270 280 290 300
AQEIMLILDE YWSQHADELG GGQVPIFYAS NLAKKCMSVF QTYVNMMNDD
310 320 330 340 350
IRKKFRDSQT NPFIFKNISY LRNLEDFQDF GPSVMLASPG MLQSGLSRDL
360 370 380 390 400
LERWCPEDKN LVLITGYSIE GTMAKFIMLE PDTIPSINNP EITIPRRCQV
410 420 430 440 450
EEISFAAHVD FQENLEFIEK ISAPNIILVH GEANPMGRLK SALLSNFASL
460 470 480 490 500
KGTDNEVHVF NPRNCVEVDL EFQGVKVAKA VGNIVNEIYK EENVEIKEEI
510 520 530 540 550
AAKIEPIKEE NEDNLDSQAE KGLVDEEEHK DIVVSGILVS DDKNFELDFL
560 570 580 590 600
SLSDLREHHP DLSTTILRER QSVRVNCKKE LIYWHILQMF GEAEVLQDDD
610 620 630 640 650
RVTNQEPKVK EESKDNLTNT GKLILQIMGD IKLTIVNTLA VVEWTQDLMN
660 670 680 690 700
DTVADSIIAI LMNVDSAPAS VKLSSHSCDD HDHNNVQSNA QGKIDEVERV
710 720 730 740 750
KQISRLFKEQ FGDCFTLFLN KDEYASNKEE TITGVVTIGK STAKIDFNNM
760 770
KILECNSNPL KGRVESLLNI GGNLVTPLC
Length:779
Mass (Da):87,674
Last modified:November 1, 1996 - v1
Checksum:i901582F0C33AD6F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17245 Genomic DNA. Translation: AAB67367.1.
BK006945 Genomic DNA. Translation: DAA09590.1.
PIRiS51413.
RefSeqiNP_013379.1. NM_001182164.1.

Genome annotation databases

EnsemblFungiiYLR277C; YLR277C; YLR277C.
GeneIDi850983.
KEGGisce:YLR277C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17245 Genomic DNA. Translation: AAB67367.1.
BK006945 Genomic DNA. Translation: DAA09590.1.
PIRiS51413.
RefSeqiNP_013379.1. NM_001182164.1.

3D structure databases

ProteinModelPortaliQ06224.
SMRiQ06224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31545. 32 interactors.
DIPiDIP-2470N.
IntActiQ06224. 23 interactors.
MINTiMINT-375457.

PTM databases

iPTMnetiQ06224.

Proteomic databases

MaxQBiQ06224.
PRIDEiQ06224.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR277C; YLR277C; YLR277C.
GeneIDi850983.
KEGGisce:YLR277C.

Organism-specific databases

EuPathDBiFungiDB:YLR277C.
SGDiS000004267. YSH1.

Phylogenomic databases

GeneTreeiENSGT00860000133746.
HOGENOMiHOG000203394.
InParanoidiQ06224.
KOiK14403.
OMAiVLIPRRC.
OrthoDBiEOG092C11WH.

Enzyme and pathway databases

BioCyciYEAST:G3O-32376-MONOMER.
ReactomeiR-SCE-77595. Processing of Intronless Pre-mRNAs.

Miscellaneous databases

PROiQ06224.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR022712. Beta_Casp.
IPR021718. CPSF73-100_C.
IPR001279. Metallo-B-lactamas.
IPR011108. RMMBL.
[Graphical view]
PfamiPF10996. Beta-Casp. 1 hit.
PF11718. CPSF73-100_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
PF07521. RMMBL. 1 hit.
[Graphical view]
SMARTiSM01027. Beta-Casp. 1 hit.
SM01098. CPSF73-100_C. 1 hit.
SM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYSH1_YEAST
AccessioniPrimary (citable) accession number: Q06224
Secondary accession number(s): D6VYS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 17400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.