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Q06219

- NR4A2_MOUSE

UniProt

Q06219 - NR4A2_MOUSE

Protein

Nuclear receptor subfamily 4 group A member 2

Gene

Nr4a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi260 – 33576Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri263 – 28321NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri299 – 32325NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: MGI
    3. protein binding Source: UniProtKB
    4. protein heterodimerization activity Source: MGI
    5. RNA polymerase II core promoter sequence-specific DNA binding Source: MGI
    6. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
    7. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    8. sequence-specific DNA binding Source: MGI
    9. steroid hormone receptor activity Source: InterPro
    10. zinc ion binding Source: InterPro

    GO - Biological processi

    1. adult locomotory behavior Source: MGI
    2. cellular response to extracellular stimulus Source: MGI
    3. cellular response to oxidative stress Source: MGI
    4. central nervous system neuron differentiation Source: MGI
    5. central nervous system projection neuron axonogenesis Source: MGI
    6. death Source: MGI
    7. dopamine biosynthetic process Source: MGI
    8. dopamine metabolic process Source: MGI
    9. dopaminergic neuron differentiation Source: UniProtKB
    10. gene expression Source: MGI
    11. general adaptation syndrome Source: MGI
    12. habenula development Source: MGI
    13. intracellular receptor signaling pathway Source: GOC
    14. negative regulation of neuron apoptotic process Source: MGI
    15. nervous system development Source: MGI
    16. neuron differentiation Source: MGI
    17. neuron maturation Source: MGI
    18. neuron migration Source: MGI
    19. positive regulation of catalytic activity Source: MGI
    20. positive regulation of transcription, DNA-templated Source: MGI
    21. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    22. post-embryonic development Source: MGI
    23. regulation of dopamine metabolic process Source: MGI
    24. regulation of gene expression Source: MGI
    25. regulation of respiratory gaseous exchange Source: MGI
    26. regulation of transcription, DNA-templated Source: MGI
    27. response to amphetamine Source: MGI
    28. response to hypoxia Source: MGI
    29. response to inorganic substance Source: MGI
    30. response to insecticide Source: MGI
    31. transcription, DNA-templated Source: UniProtKB

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor subfamily 4 group A member 2
    Alternative name(s):
    NUR-related factor 1
    Orphan nuclear receptor NURR1
    Gene namesi
    Name:Nr4a2
    Synonyms:Nurr1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1352456. Nr4a2.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus
    Note: Mostly nuclear; oxidative stress promotes cytoplasmic localization.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 598598Nuclear receptor subfamily 4 group A member 2PRO_0000053719Add
    BLAST

    Proteomic databases

    PRIDEiQ06219.

    PTM databases

    PhosphoSiteiQ06219.

    Expressioni

    Tissue specificityi

    Brain.

    Developmental stagei

    Expression begins in the embryo, increases in neonates and decreases in the adult.

    Gene expression databases

    ArrayExpressiQ06219.
    BgeeiQ06219.
    CleanExiMM_NR4A2.
    GenevestigatoriQ06219.

    Interactioni

    Subunit structurei

    Interacts with SFPQ, NCOR2, SIN3A and HADC1. The interaction with NCOR2 increases in the absence of PITX3. Interacts with PER2.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Rcor1Q8CFE35EBI-2337255,EBI-2337309
    RelaQ042072EBI-2337255,EBI-644400

    Protein-protein interaction databases

    BioGridi201880. 1 interaction.
    IntActiQ06219. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06219.
    SMRiQ06219. Positions 261-598.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni409 – 45951Ligand-bindingSequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi287 – 31428Bipartite nuclear localization signal (NLS1)By similarityAdd
    BLAST
    Motifi338 – 35013Nuclear localization signal (NLS1)By similarityAdd
    BLAST
    Motifi443 – 45210nuclear export sequence (NES1)By similarity
    Motifi568 – 57710nuclear export sequence (NES2)By similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi80 – 13859Gln-richAdd
    BLAST
    Compositional biasi127 – 233107Pro-richAdd
    BLAST
    Compositional biasi346 – 39146Pro-richAdd
    BLAST

    Domaini

    the ligand-binding domain (LBD) contains no cavity as a result of the tight packing of side chains from several bulky hydrophobic residues in the region normally occupied by ligands. NR4A2 lacks a 'classical' binding site for coactivators By similarity.By similarity

    Sequence similaritiesi

    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri263 – 28321NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri299 – 32325NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG245120.
    HOGENOMiHOG000230925.
    HOVERGENiHBG052663.
    InParanoidiQ06219.
    KOiK08558.
    OMAiMWEDPGS.
    OrthoDBiEOG75XGKJ.
    PhylomeDBiQ06219.
    TreeFamiTF315430.

    Family and domain databases

    Gene3Di1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProiIPR003070. Nuc_orph_rcpt.
    IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR003073. NURR_rcpt.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PRINTSiPR01284. NUCLEARECPTR.
    PR01287. NURRNUCRCPTR.
    PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 1 hit.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform NURR1 (identifier: Q06219-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPCVQAQYGS SPQGASPASQ SYSYHSSGEY SSDFLTPEFV KFSMDLTNTE    50
    ITATTSLPSF STFMDNYSTG YDVKPPCLYQ MPLSGQQSSI KVEDIQMHNY 100
    QQHSHLPPQS EEMMPHSGSV YYKPSSPPTP STPSFQVQHS PMWDDPGSLH 150
    NFHQNYVATT HMIEQRKTPV SRLSLFSFKQ SPPGTPVSSC QMRFDGPLHV 200
    PMNPEPAGSH HVVDGQTFAV PNPIRKPASM GFPGLQIGHA SQLLDTQVPS 250
    PPSRGSPSNE GLCAVCGDNA ACQHYGVRTC EGCKGFFKRT VQKNAKYVCL 300
    ANKNCPVDKR RRNRCQYCRF QKCLAVGMVK EVVRTDSLKG RRGRLPSKPK 350
    SPQDPSPPSP PVSLISALVR AHVDSNPAMT SLDYSRFQAN PDYQMSGDDT 400
    QHIQQFYDLL TGSMEIIRGW AEKIPGFADL PKADQDLLFE SAFLELFVLR 450
    LAYRSNPVEG KLIFCNGVVL HRLQCVRGFG EWIDSIVEFS SNLQNMNIDI 500
    SAFSCIAALA MVTERHGLKE PKRVEELQNK IVNCLKDHVT FNNGGLNRPN 550
    YLSKLLGKLP ELRTLCTQGL QRIFYLKLED LVPPPAIIDK LFLDTLPF 598
    Length:598
    Mass (Da):66,593
    Last modified:June 1, 1994 - v1
    Checksum:iD1AC5EB2FFB8D366
    GO
    Isoform NURR1A (identifier: Q06219-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         455-455: S → I
         456-598: Missing.

    Show »
    Length:455
    Mass (Da):50,458
    Checksum:iA3B91CFBAAD62D13
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei455 – 4551S → I in isoform NURR1A. CuratedVSP_003710
    Alternative sequencei456 – 598143Missing in isoform NURR1A. CuratedVSP_003711Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S53744 mRNA. Translation: AAB25138.1.
    U86783 Genomic DNA. Translation: AAC53153.1.
    U86783 Genomic DNA. Translation: AAC53154.1.
    CCDSiCCDS16043.1. [Q06219-1]
    PIRiA46225.
    RefSeqiNP_001132981.1. NM_001139509.1. [Q06219-1]
    NP_038641.1. NM_013613.2. [Q06219-1]
    UniGeneiMm.3507.

    Genome annotation databases

    EnsembliENSMUST00000028166; ENSMUSP00000028166; ENSMUSG00000026826. [Q06219-1]
    ENSMUST00000112629; ENSMUSP00000108248; ENSMUSG00000026826. [Q06219-1]
    GeneIDi18227.
    KEGGimmu:18227.
    UCSCiuc008jrw.2. mouse. [Q06219-1]
    uc012bvn.1. mouse. [Q06219-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S53744 mRNA. Translation: AAB25138.1 .
    U86783 Genomic DNA. Translation: AAC53153.1 .
    U86783 Genomic DNA. Translation: AAC53154.1 .
    CCDSi CCDS16043.1. [Q06219-1 ]
    PIRi A46225.
    RefSeqi NP_001132981.1. NM_001139509.1. [Q06219-1 ]
    NP_038641.1. NM_013613.2. [Q06219-1 ]
    UniGenei Mm.3507.

    3D structure databases

    ProteinModelPortali Q06219.
    SMRi Q06219. Positions 261-598.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201880. 1 interaction.
    IntActi Q06219. 3 interactions.

    PTM databases

    PhosphoSitei Q06219.

    Proteomic databases

    PRIDEi Q06219.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028166 ; ENSMUSP00000028166 ; ENSMUSG00000026826 . [Q06219-1 ]
    ENSMUST00000112629 ; ENSMUSP00000108248 ; ENSMUSG00000026826 . [Q06219-1 ]
    GeneIDi 18227.
    KEGGi mmu:18227.
    UCSCi uc008jrw.2. mouse. [Q06219-1 ]
    uc012bvn.1. mouse. [Q06219-2 ]

    Organism-specific databases

    CTDi 4929.
    MGIi MGI:1352456. Nr4a2.

    Phylogenomic databases

    eggNOGi NOG245120.
    HOGENOMi HOG000230925.
    HOVERGENi HBG052663.
    InParanoidi Q06219.
    KOi K08558.
    OMAi MWEDPGS.
    OrthoDBi EOG75XGKJ.
    PhylomeDBi Q06219.
    TreeFami TF315430.

    Miscellaneous databases

    ChiTaRSi NR4A2. mouse.
    NextBioi 293656.
    PROi Q06219.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q06219.
    Bgeei Q06219.
    CleanExi MM_NR4A2.
    Genevestigatori Q06219.

    Family and domain databases

    Gene3Di 1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProi IPR003070. Nuc_orph_rcpt.
    IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR003073. NURR_rcpt.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PRINTSi PR01284. NUCLEARECPTR.
    PR01287. NURRNUCRCPTR.
    PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 1 hit.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a new brain-specific transcription factor, NURR1."
      Law S.W., Conneely O.M., DeMayo F.J., O'Malley B.W.
      Mol. Endocrinol. 6:2129-2135(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NURR1).
      Tissue: Brain.
    2. "Organization, sequence, chromosomal localization, and promoter identification of the mouse orphan nuclear receptor Nurr1 gene."
      Castillo S.O., Xiao Q., Lyu M.S., Kozak C.A., Nikodem V.M.
      Genomics 41:250-257(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
    3. "Pitx3 potentiates Nurr1 in dopamine neuron terminal differentiation through release of SMRT-mediated repression."
      Jacobs F.M., van Erp S., van der Linden A.J., von Oerthel L., Burbach J.P., Smidt M.P.
      Development 136:531-540(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SFPQ; NCOR2; SIN3A AND HDAC1.
    4. "The mammalian clock component PERIOD2 coordinates circadian output by interaction with nuclear receptors."
      Schmutz I., Ripperger J.A., Baeriswyl-Aebischer S., Albrecht U.
      Genes Dev. 24:345-357(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PER2.

    Entry informationi

    Entry nameiNR4A2_MOUSE
    AccessioniPrimary (citable) accession number: Q06219
    Secondary accession number(s): O08690
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3