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Q06219 (NR4A2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear receptor subfamily 4 group A member 2
Alternative name(s):
NUR-related factor 1
Orphan nuclear receptor NURR1
Gene names
Name:Nr4a2
Synonyms:Nurr1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length598 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Ref.3

Subunit structure

Interacts with SFPQ, NCOR2, SIN3A and HADC1. The interaction with NCOR2 increases in the absence of PITX3. Interacts with PER2. Ref.3 Ref.4

Subcellular location

Cytoplasm By similarity. Nucleus. Note: Mostly nuclear; oxidative stress promotes cytoplasmic localization By similarity.

Tissue specificity

Brain.

Developmental stage

Expression begins in the embryo, increases in neonates and decreases in the adult.

Domain

the ligand-binding domain (LBD) contains no cavity as a result of the tight packing of side chains from several bulky hydrophobic residues in the region normally occupied by ligands. NR4A2 lacks a 'classical' binding site for coactivators By similarity.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR4 subfamily.

Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionReceptor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadult locomotory behavior

Inferred from mutant phenotype PubMed 12385813. Source: MGI

cellular response to extracellular stimulus

Inferred from direct assay PubMed 8961274. Source: MGI

cellular response to oxidative stress

Inferred from mutant phenotype PubMed 20566846. Source: MGI

central nervous system neuron differentiation

Inferred from mutant phenotype PubMed 11113533. Source: MGI

central nervous system projection neuron axonogenesis

Inferred from mutant phenotype PubMed 10585298. Source: MGI

death

Inferred from mutant phenotype PubMed 9608532. Source: MGI

dopamine biosynthetic process

Inferred from mutant phenotype PubMed 16782508PubMed 9608532. Source: MGI

dopamine metabolic process

Inferred from mutant phenotype PubMed 12385813. Source: MGI

dopaminergic neuron differentiation

Inferred from direct assay Ref.3. Source: UniProtKB

gene expression

Inferred from mutant phenotype PubMed 10465447. Source: MGI

general adaptation syndrome

Inferred from mutant phenotype PubMed 12385813. Source: MGI

habenula development

Inferred from mutant phenotype PubMed 19906978. Source: MGI

intracellular receptor signaling pathway

Traceable author statement PubMed 9092472. Source: GOC

negative regulation of neuron apoptotic process

Inferred from mutant phenotype PubMed 10585298PubMed 20566846. Source: MGI

nervous system development

Inferred from mutant phenotype PubMed 11749040PubMed 9092472. Source: MGI

neuron differentiation

Inferred from mutant phenotype PubMed 14622207PubMed 9520484. Source: MGI

neuron maturation

Inferred from mutant phenotype PubMed 10585298. Source: MGI

neuron migration

Inferred from mutant phenotype PubMed 10585298. Source: MGI

positive regulation of catalytic activity

Inferred from mutant phenotype PubMed 11517332. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 14671317PubMed 15155786PubMed 17394463. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 8961274. Source: MGI

post-embryonic development

Inferred from mutant phenotype PubMed 9608532. Source: MGI

regulation of dopamine metabolic process

Inferred from direct assay PubMed 12915123. Source: MGI

regulation of gene expression

Inferred from mutant phenotype PubMed 19515692. Source: MGI

regulation of respiratory gaseous exchange

Inferred from mutant phenotype PubMed 14742729. Source: MGI

regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 19906978. Source: MGI

response to amphetamine

Inferred from mutant phenotype PubMed 12385813. Source: MGI

response to hypoxia

Inferred from mutant phenotype PubMed 14742729. Source: MGI

response to inorganic substance

Inferred from sequence orthology Ref.1. Source: MGI

response to insecticide

Inferred from sequence orthology PubMed 8737662. Source: MGI

transcription, DNA-templated

Inferred from direct assay Ref.3. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay PubMed 11749040PubMed 18388149. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 14671317. Source: MGI

RNA polymerase II core promoter sequence-specific DNA binding

Inferred from direct assay PubMed 19515692. Source: MGI

ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity

Traceable author statement PubMed 9092472. Source: MGI

protein binding

Inferred from physical interaction Ref.3Ref.4. Source: UniProtKB

protein heterodimerization activity

Inferred from physical interaction PubMed 8961274. Source: MGI

sequence-specific DNA binding

Inferred from direct assay PubMed 8961274. Source: MGI

steroid hormone receptor activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform NURR1 (identifier: Q06219-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform NURR1A (identifier: Q06219-2)

The sequence of this isoform differs from the canonical sequence as follows:
     455-455: S → I
     456-598: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 598598Nuclear receptor subfamily 4 group A member 2
PRO_0000053719

Regions

DNA binding260 – 33576Nuclear receptor
Zinc finger263 – 28321NR C4-type
Zinc finger299 – 32325NR C4-type
Region409 – 45951Ligand-binding Potential
Motif287 – 31428Bipartite nuclear localization signal (NLS1) By similarity
Motif338 – 35013Nuclear localization signal (NLS1) By similarity
Motif443 – 45210nuclear export sequence (NES1) By similarity
Motif568 – 57710nuclear export sequence (NES2) By similarity
Compositional bias80 – 13859Gln-rich
Compositional bias127 – 233107Pro-rich
Compositional bias346 – 39146Pro-rich

Natural variations

Alternative sequence4551S → I in isoform NURR1A.
VSP_003710
Alternative sequence456 – 598143Missing in isoform NURR1A.
VSP_003711

Sequences

Sequence LengthMass (Da)Tools
Isoform NURR1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: D1AC5EB2FFB8D366

FASTA59866,593
        10         20         30         40         50         60 
MPCVQAQYGS SPQGASPASQ SYSYHSSGEY SSDFLTPEFV KFSMDLTNTE ITATTSLPSF 

        70         80         90        100        110        120 
STFMDNYSTG YDVKPPCLYQ MPLSGQQSSI KVEDIQMHNY QQHSHLPPQS EEMMPHSGSV 

       130        140        150        160        170        180 
YYKPSSPPTP STPSFQVQHS PMWDDPGSLH NFHQNYVATT HMIEQRKTPV SRLSLFSFKQ 

       190        200        210        220        230        240 
SPPGTPVSSC QMRFDGPLHV PMNPEPAGSH HVVDGQTFAV PNPIRKPASM GFPGLQIGHA 

       250        260        270        280        290        300 
SQLLDTQVPS PPSRGSPSNE GLCAVCGDNA ACQHYGVRTC EGCKGFFKRT VQKNAKYVCL 

       310        320        330        340        350        360 
ANKNCPVDKR RRNRCQYCRF QKCLAVGMVK EVVRTDSLKG RRGRLPSKPK SPQDPSPPSP 

       370        380        390        400        410        420 
PVSLISALVR AHVDSNPAMT SLDYSRFQAN PDYQMSGDDT QHIQQFYDLL TGSMEIIRGW 

       430        440        450        460        470        480 
AEKIPGFADL PKADQDLLFE SAFLELFVLR LAYRSNPVEG KLIFCNGVVL HRLQCVRGFG 

       490        500        510        520        530        540 
EWIDSIVEFS SNLQNMNIDI SAFSCIAALA MVTERHGLKE PKRVEELQNK IVNCLKDHVT 

       550        560        570        580        590 
FNNGGLNRPN YLSKLLGKLP ELRTLCTQGL QRIFYLKLED LVPPPAIIDK LFLDTLPF 

« Hide

Isoform NURR1A [UniParc].

Checksum: A3B91CFBAAD62D13
Show »

FASTA45550,458

References

[1]"Identification of a new brain-specific transcription factor, NURR1."
Law S.W., Conneely O.M., DeMayo F.J., O'Malley B.W.
Mol. Endocrinol. 6:2129-2135(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NURR1).
Tissue: Brain.
[2]"Organization, sequence, chromosomal localization, and promoter identification of the mouse orphan nuclear receptor Nurr1 gene."
Castillo S.O., Xiao Q., Lyu M.S., Kozak C.A., Nikodem V.M.
Genomics 41:250-257(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
[3]"Pitx3 potentiates Nurr1 in dopamine neuron terminal differentiation through release of SMRT-mediated repression."
Jacobs F.M., van Erp S., van der Linden A.J., von Oerthel L., Burbach J.P., Smidt M.P.
Development 136:531-540(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SFPQ; NCOR2; SIN3A AND HDAC1.
[4]"The mammalian clock component PERIOD2 coordinates circadian output by interaction with nuclear receptors."
Schmutz I., Ripperger J.A., Baeriswyl-Aebischer S., Albrecht U.
Genes Dev. 24:345-357(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PER2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S53744 mRNA. Translation: AAB25138.1.
U86783 Genomic DNA. Translation: AAC53153.1.
U86783 Genomic DNA. Translation: AAC53154.1.
CCDSCCDS16043.1. [Q06219-1]
PIRA46225.
RefSeqNP_001132981.1. NM_001139509.1. [Q06219-1]
NP_038641.1. NM_013613.2. [Q06219-1]
UniGeneMm.3507.

3D structure databases

ProteinModelPortalQ06219.
SMRQ06219. Positions 261-598.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201880. 1 interaction.
IntActQ06219. 3 interactions.

PTM databases

PhosphoSiteQ06219.

Proteomic databases

PRIDEQ06219.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028166; ENSMUSP00000028166; ENSMUSG00000026826. [Q06219-1]
ENSMUST00000112629; ENSMUSP00000108248; ENSMUSG00000026826. [Q06219-1]
GeneID18227.
KEGGmmu:18227.
UCSCuc008jrw.2. mouse. [Q06219-1]
uc012bvn.1. mouse. [Q06219-2]

Organism-specific databases

CTD4929.
MGIMGI:1352456. Nr4a2.

Phylogenomic databases

eggNOGNOG245120.
HOGENOMHOG000230925.
HOVERGENHBG052663.
InParanoidQ06219.
KOK08558.
OMAMWEDPGS.
OrthoDBEOG75XGKJ.
PhylomeDBQ06219.
TreeFamTF315430.

Gene expression databases

ArrayExpressQ06219.
BgeeQ06219.
CleanExMM_NR4A2.
GenevestigatorQ06219.

Family and domain databases

Gene3D1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProIPR003070. Nuc_orph_rcpt.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR003073. NURR_rcpt.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSPR01284. NUCLEARECPTR.
PR01287. NURRNUCRCPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 1 hit.
PROSITEPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNR4A2. mouse.
NextBio293656.
PROQ06219.
SOURCESearch...

Entry information

Entry nameNR4A2_MOUSE
AccessionPrimary (citable) accession number: Q06219
Secondary accession number(s): O08690
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 9, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot